{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T10:00:28Z","timestamp":1780999228285,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head & Neck"],"published-print":{"date-parts":[[2026,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n Pharyngeal squamous cell carcinoma (PSCC) is an aggressive subtype of head and neck squamous cell carcinoma (HNSCC) with poor prognosis and low survival rates. Immune checkpoint inhibitors (ICIs) show promise, but less than 20% of HNSCC patients respond positively. Targeted radionuclide therapy (TRT) combines radionuclides with monoclonal antibodies to target tumor cells. This study created a reliable animal model of PSCC for evaluating the therapeutic efficacy of\n 131<\/jats:sup>\n I\u2010aPD\u2010L1.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Nude mice were subcutaneously implanted with FaDu cells\u2014a human PSCC cell line characterized by high PD\u2010L1 expression. The synthesis of\n 131<\/jats:sup>\n I\u2010aPD\u2010L1 was optimized by varying labeling conditions, achieving a labeling efficiency of over 90%. Mice were divided into experimental and control groups; the experimental group received a single intravenous injection of 500\u2009\u03bcCi\n 131<\/jats:sup>\n I\u2010aPD\u2010L1. Accumulation of\n 131<\/jats:sup>\n I\u2010aPD\u2010L1 in tumor tissues was confirmed by animal single\u2010photon emission computed tomography (SPECT). Tumor volume and mouse body weight were measured every 3\u2009days for 30\u2009days. At the end of the study, tumor tissues were stained for histological examination and immunohistochemical analysis of Bcl\u20102 and Caspase\u20103 expression levels. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was also performed on tumor tissues.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n SPECT verified a significant accumulation of\n 131<\/jats:sup>\n I in FaDu tumor tissue. The experimental group exhibited significantly slower tumor volume increase compared to the control group (\n t<\/jats:italic>\n \u2009=\u20092.37,\n p<\/jats:italic>\n \u2009<\u20090.05). Additionally, a significant reduction in body weight was observed in the\n 131<\/jats:sup>\n I\u2010aPD\u2010L1 group compared to the control group (\n t<\/jats:italic>\n \u2009=\u20095.624,\n p<\/jats:italic>\n \u2009<\u20090.01). HE staining showed extensive tumor necrosis in the experimental group. Immunohistochemical analysis revealed negative Bcl\u20102 expression and higher caspase\u20103 expression in the experimental group, indicating enhanced apoptosis and necrosis in tumor cells. Furthermore, TUNEL assay further confirmed that\n 131<\/jats:sup>\n I exerted cytotoxic effects by inducing DNA fragmentation.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Collectively, our findings demonstrate the promising therapeutic potential of\n 131<\/jats:sup>\n I\u2010aPD\u2010L1 for PSCC, particularly in patients with drug resistance or recurrent head and neck tumors. However, the use of nude mice may have impacted the full therapeutic efficacy and synergistic potential observed with immunotherapy. Future studies should utilize immunocompetent models to better assess the probe's therapeutic impact and to explore its synergistic effects with immunotherapy and reduce the dose of\n 131<\/jats:sup>\n I to mitigate its toxic effects.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/hed.70146","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T09:06:44Z","timestamp":1766740004000},"page":"1409-1415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted Radionuclide Therapy With\n \n 131<\/sup>\n I\n <\/scp>\n \u2010Labeled Anti\u2010\n PD<\/scp>\n \u2010\n L1<\/scp>\n Antibody Suppresses Pharyngeal Squamous Cell Carcinoma in the Animal Model"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1066-3532","authenticated-orcid":false,"given":"Ming","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, The Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9761-9915","authenticated-orcid":false,"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangqian","family":"Tu","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Zhejiang Chinese Medical University Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Handan","family":"Zheng","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Zhejiang Chinese Medical University Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1303-5224","authenticated-orcid":false,"given":"Xiaoxi","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine The Second Hospital of Anhui Medical University Hefei Anhui Province 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