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The\n                      <jats:italic>EWSR1::ATF<\/jats:italic>\n                      1 fusion gene, resulting from a translocation between chromosomes 12 and 22, is frequently observed in hyalinizing clear cell carcinoma (HCCC). However, the role of this fusion gene in HCCC oncogenesis remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We generated\n                      <jats:italic>EWSR1::ATF1<\/jats:italic>\n                      transgenic mice by introducing an\n                      <jats:italic>EWSR1::ATF1<\/jats:italic>\n                      fusion gene with a breakpoint in HCCC. These mice were crossed with\n                      <jats:italic>Lama parotid secretory protein (PSP)\u2010Cre<\/jats:italic>\n                      transgenic mice, expressing Cre recombinase in salivary glands, to produce salivary gland\u2010specific\n                      <jats:italic>EWSR1::ATF1<\/jats:italic>\n                      transgenic mice. Tumor samples were analyzed using Western blotting, histopathology, and RNA sequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We generated salivary gland\u2010specific\n                      <jats:italic>EWSR1::ATF1<\/jats:italic>\n                      transgenic mice, and tumors developed in major salivary glands in three mice. These tumors exhibited more malignant characteristics than human HCCC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These results suggest that salivary gland\u2010specific expression of\n                      <jats:italic>EWSR1::ATF1<\/jats:italic>\n                      plays an important role in malignant tumor formation in major salivary glands.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hed.70289","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:40:51Z","timestamp":1777102851000},"page":"2733-2744","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of Salivary Gland Tumors Arising in Salivary Gland\u2010Specific\n                    <i>EWSR1::ATF1<\/i>\n                    Transgenic Mice"],"prefix":"10.1002","volume":"48","author":[{"given":"Yuri","family":"Hirai","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery School of Medicine, Yokohama City University  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Utsumi","sequence":"additional","affiliation":[{"name":"Department of Anatomic Pathology Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Sano","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery School of Medicine, Yokohama City University  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsu","family":"Kuwahara","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery School of Medicine, Yokohama City University  Yokohama 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