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To date, their expression in multiple myeloma (MM) patients has mostly been analyzed at the RNA level. In the present study, we quantified for the first time the protein expression of the Bcl2\u2010family members using a capillary electrophoresis immunoassay in 120 newly diagnosed MM patients, aged \u226465 years, treated in the context of the PETHEMA\/GEM2012 study. We found that the pattern of expression of Bcl\u20102 family proteins was highly heterogeneous among patients. Although cases with t(11;14) had significantly higher levels of Bcl\u20102\/Bcl\u2010xL and Bcl\u20102+Bim+Bax\/Bcl\u2010xL ratios than those without t(11;14), the presence of this translocation was not synonymous with such high levels of expression. Conversely, some patients with other genetic alterations also showed higher levels of those ratios. Survival analysis revealed that the high expression of Bad and Puma proteins was associated with significantly longer overall survival (<jats:italic>p<\/jats:italic>\u2009=\u20090.001 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001, respectively). Bcl\u20102 protein ratios predicting sensitivity to venetoclax <jats:italic>in vitro<\/jats:italic> were also able to distinguish patients with shorter time to progression after triplet\u2010based induction therapy and ASCT. This is the first study to assess the expression of the most important Bcl\u20102 family proteins by a quantitative method in a large set of MM patients according to their cytogenetic abnormalities. We shed light on the impact of these proteins on MM prognosis, which could help to consider the levels of proteins involved in apoptosis in the development of new therapeutic strategies.<\/jats:p>","DOI":"10.1002\/hem3.70036","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T00:38:41Z","timestamp":1734136721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Expression profile of Bcl\u20102 family proteins in newly diagnosed multiple myeloma patients"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8167-6410","authenticated-orcid":false,"given":"Cristina","family":"De Ram\u00f3n","sequence":"first","affiliation":[{"name":"Department of Hematology, University Hospital of Salamanca Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeta A.","family":"Rojas","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital of Salamanca Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irena","family":"Misiewicz\u2010Krzeminska","sequence":"additional","affiliation":[{"name":"Department of Experimental Hematology Institute of Hematology and Transfusion Medicine Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignacio J.","family":"Cardona\u2010Benavides","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital of Salamanca Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam","family":"Cuadrado","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital of Salamanca Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Isidro","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital of Salamanca Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda\u2010Jos\u00e9","family":"Calasanz","sequence":"additional","affiliation":[{"name":"Department of Hematology Cl\u00ednica Universidad de Navarra, Centro de Investigaciones Biom\u00e9dicas Aplicadas (CIMA), Instituto de Investigaci\u00f3n Sanitaria de Navarra (IdiSNA) Pamplona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer (CIBERONC)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Spanish National Cancer Research Center (CNIO) Madrid Spain"},{"name":"Department of Hematology, University Hospital 12 de Octubre; Medicine Department Complutense University Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram\u00f3n","family":"Garc\u00eda\u2010Sanz","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital of Salamanca Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer (CIBERONC)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noemi","family":"Puig","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital of Salamanca Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Cancer Research Center\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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