{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T23:34:17Z","timestamp":1783035257758,"version":"3.54.6"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HemaSphere"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chimeric antigen receptor (CAR)\u2010T\u2010cell therapy has improved response rates in relapsed\/refractory multiple myeloma (RRMM), yet long\u2010term disease control remains limited, with only 20% of patients remaining progression\u2010free at 5 years. Therefore, identifying early predictors of treatment success is critical. Here, we used next\u2010generation flow cytometry to analyze T\u2010cell populations in blood at leukapheresis, before infusion, and post\u2010anti\u2010BCMA CAR\u2010T infusion, together with CAR\u2010T\u2010cell kinetics and phenotypes before, during, and after the expansion peak, in 53 RRMM patients. Anti\u2010BCMA CAR\u2010T cells peaked at Day +14 (72%), comprising &gt;65 distinct populations, predominantly central memory (CM) T\u2010helper (Th) 1 and Th1\/2 CAR\u2010TCD4\n                    <jats:sup>+<\/jats:sup>\n                    and CM CAR\u2010TCD8\n                    <jats:sup>+<\/jats:sup>\n                    cells. Multivariate analyses revealed that higher frequencies of TCD4\n                    <jats:sup>+<\/jats:sup>\n                    naive and Th22 transitional memory (TM) cells at leukapheresis, together with circulating tumor plasma cells (CTPCs) before infusion, but none of the specific CAR\u2010T\u2010cell populations, were predictors of progression\u2010free survival (PFS). Based on these variables, we built a risk score that together with disease stage (International Staging System\u2010Revised [ISS\u2010R]) independently predicted, before CAR\u2010T\u2010cell infusion, which RRMM patients were most likely to benefit from anti\u2010BCMA CAR\u2010T therapy. These findings suggest that long\u2010term PFS is primarily influenced by the patient's immune landscape and the tumor burden rather than anti\u2010BCMA CAR\u2010T\u2010cell properties.\n                  <\/jats:p>","DOI":"10.1002\/hem3.70277","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T02:57:30Z","timestamp":1766977050000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of the kinetics of circulating anti\u2010BCMA CAR\u2010T cells and normal lymphocytes on the outcome of MM patients"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5507-3985","authenticated-orcid":false,"given":"Sara","family":"Guti\u00e9rrez\u2010Herrero","sequence":"first","affiliation":[{"name":"Translational and Clinical Research Program, Centro de Investigaci\u00f3n del C\u00e1ncer and Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Consejo Superior de Investigaciones Cient\u00edficas (CSIC) University of Salamanca (Universidad de Salamanca) Salamanca Spain"},{"name":"Cytometry Service (NUCLEUS) Salamanca Spain"},{"name":"Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Department of Medicine University of Salamanca (Universidad de Salamanca) Salamanca Spain"},{"name":"CIBERONC University of Salamanca and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7996-570X","authenticated-orcid":false,"given":"Lourdes","family":"Mart\u00edn\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Program, Centro de Investigaci\u00f3n del C\u00e1ncer and Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Consejo Superior de Investigaciones Cient\u00edficas (CSIC) University of Salamanca (Universidad de Salamanca) Salamanca Spain"},{"name":"Cytometry Service (NUCLEUS) Salamanca Spain"},{"name":"Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Department of Medicine University of Salamanca (Universidad de Salamanca) Salamanca Spain"},{"name":"CIBERONC University of Salamanca and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8719-7165","authenticated-orcid":false,"given":"Mar\u00eda","family":"Herrero\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Program, Centro de Investigaci\u00f3n del C\u00e1ncer and Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer (IBMCC), Consejo Superior de Investigaciones Cient\u00edficas (CSIC) University of Salamanca (Universidad de Salamanca) Salamanca Spain"},{"name":"Cytometry Service (NUCLEUS) Salamanca 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