{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:56:27Z","timestamp":1790679387241,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HemaSphere"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Relapsed\/refractory (R\/R) B\u2010cell lymphomas (B\u2010NHL) remain incurable, with limited predictive biomarkers to guide immunotherapy. Preclinical platforms that faithfully preserve the immune context and the viability of patient samples are critically needed for relevant high\u2010throughput drug testing. Here, we present a 3D ex vivo platform for immunotherapy screening that generates patient\u2010derived lymphoma spheroids (PDLS) a scaffold\u2010 and matrix\u2010free 3D model derived from peripheral blood or lymph node biopsies of patients, including R\/R follicular lymphoma, transformed follicular lymphoma, or diffuse large B\u2010cell lymphoma. PDLS maintain the cellular composition, T\u2010cell activation, and immune escape profiles of the original tumors, supporting multiplexed screening of single\u2010agent and combination therapies within clinically relevant time frames. This scalable model recapitulates immune\u2013tumor cell interactions, provides rapid assessment of immunotherapy efficacy, and bridges preclinical research with clinical application in aggressive B\u2010cell lymphomas. By integrating spatial profiling and single\u2010cell RNA sequencing, PDLS can further evolve into patient\u2010specific 3D tumor avatars that support precision oncology approaches in aggressive B\u2010NHL.<\/jats:p>","DOI":"10.1002\/hem3.70397","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T10:48:46Z","timestamp":1788259726000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["3D ex vivo platform for high\u2010throughput immunotherapy screening in relapsed\/refractory lymphoma patients"],"prefix":"10.1002","volume":"10","author":[{"given":"Fabien","family":"Gava","sequence":"first","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"},{"name":"Department of Pathology IUCT\u2010Oncopole Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4237-2668","authenticated-orcid":false,"given":"Paul","family":"Marcoux","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neus","family":"Serrat","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 Cl\u00ednic per a la Recerca Biom\u00e8dica\u2014Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer Barcelona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red\u2010Oncolog\u00eda (CIBERONC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Gravelle","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"},{"name":"Department of Pathology IUCT\u2010Oncopole Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Quertinmont","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Tosolini","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Faria","sequence":"additional","affiliation":[{"name":"Oncology Department UNIL\u2010CHUV Bernhard Gentner laboratory, Agora Research Center Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan G.","family":"Valero","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 Cl\u00ednic per a la Recerca Biom\u00e8dica\u2014Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer Barcelona Spain"},{"name":"Department of Hematology Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Schniederjohann","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf D\u00fcsseldorf Germany"},{"name":"Center for Integrated Oncology Aachen\u2010Bonn\u2010Cologne\u2010D\u00fcsseldorf (CIO ABCD) D\u00fcsseldorf Germany"},{"name":"Molecular Medicine Partnership Unit (MMPU) Heidelberg Germany"},{"name":"Department of Medicine V Heidelberg University Hospital Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy","family":"Quelen","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny","family":"Bouquet","sequence":"additional","affiliation":[{"name":"F.Hoffmann\u2010La Roche Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catia","family":"Fonseca","sequence":"additional","affiliation":[{"name":"F.Hoffmann\u2010La Roche Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Herter","sequence":"additional","affiliation":[{"name":"Roche Pharma Research and Early Development Roche Innovation Center Zu\u0308rich Schlieren Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura C.","family":"Deak","sequence":"additional","affiliation":[{"name":"Roche Pharma Research and Early Development Roche Innovation Center Zu\u0308rich Schlieren Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron","family":"McCord","sequence":"additional","affiliation":[{"name":"Genentech San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene","family":"Chiang","sequence":"additional","affiliation":[{"name":"Genentech San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter\u2010Martin","family":"Bruch","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf D\u00fcsseldorf Germany"},{"name":"Center for Integrated Oncology Aachen\u2010Bonn\u2010Cologne\u2010D\u00fcsseldorf (CIO ABCD) D\u00fcsseldorf Germany"},{"name":"Molecular Medicine Partnership Unit (MMPU) Heidelberg Germany"},{"name":"Department of Medicine V Heidelberg University Hospital Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4102-7261","authenticated-orcid":false,"given":"Lo\u00efc","family":"Ysebaert","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"},{"name":"Department of Hematology IUCT\u2010Oncopole Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"P\u00e9rez\u2010Gal\u00e1n","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3 Cl\u00ednic per a la Recerca Biom\u00e8dica\u2014Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer Barcelona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red\u2010Oncolog\u00eda (CIBERONC) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Laurent","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"},{"name":"Department of Pathology IUCT\u2010Oncopole Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4079-4872","authenticated-orcid":false,"given":"Christine","family":"Bezombes","sequence":"additional","affiliation":[{"name":"Univ Toulouse, INSERM, CRCT Toulouse France"},{"name":"Institut Claudius Regaud \u2010 IUCT\u2010Oncopole, imCORE site Toulouse France"},{"name":"Laboratoire d'Excellence \u2018TOUCAN\u20102' Toulouse France"},{"name":"Institut Carnot Lymphome CALYM Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015851"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa011795"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-01-0016"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-08-038257"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-019-0132-x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2020-063439"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.13697"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2025.03.005"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-021-03897-9"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/HS9.0000000000000932"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.70114"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021005400"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020003949"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2023-007156"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-024-01041-7"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-55098-w"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2024.01.012"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.71.2083"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1403189"},{"issue":"1","key":"e_1_2_11_21_1","first-page":"1","article-title":"Web\u2010based analysis and publication of flow cytometry experiments","volume":"53","author":"Kotecha N","year":"2010","journal-title":"Curr Protoc Cytom"},{"key":"e_1_2_11_22_1","article-title":"Spatialproteomics\u2014an interoperable toolbox for analyzing highly multiplexed fluorescence image data","author":"Meyer\u2010Bender M","year":"2025","journal-title":"bioRxiv"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-4188-0_16"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-06-741041"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-024-01061-1"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019000847"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14011"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1674"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07121-9"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2022-005548"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-18-0455"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2020-134782"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2022-159941"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2023023381"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-02-205500"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2025031309"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-023-01495-3"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020002580"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-03489-3"},{"key":"e_1_2_11_41_1","unstructured":"GimenezR.Patient\u2010derived follicular lymphoma tumoroids: a tool to recapitulate biological features of low and high\u2010grade lymphoma. In: ASH; 2024. Accessed April 8 2026.https:\/\/ash.confex.com\/ash\/2024\/webprogram\/Paper198454.html"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01398-3"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medj.2023.04.003"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-024-02037-1"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06299-6"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-023-00645-5"}],"container-title":["HemaSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hem3.70397","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hem3.70397","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hem3.70397","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:10:41Z","timestamp":1790676641000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hem3.70397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":45,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/hem3.70397"],"URL":"https:\/\/doi.org\/10.1002\/hem3.70397","archive":["Portico"],"relation":{},"ISSN":["2572-9241","2572-9241"],"issn-type":[{"value":"2572-9241","type":"print"},{"value":"2572-9241","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2025-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70397"}}