{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T14:15:23Z","timestamp":1773497723390,"version":"3.50.1"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":12296,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>A radioimmunoassay (RIA) was established for the detection of antimitochondrial autoantibodies (AMAs) in patient sera. AMAs were detected by RIA in 12 of 14 patients with primary biliary cirrhosis and in 3 of 29 patients with chronic active hepatitis. AMAs were detected by indirect immunofluorescence in all sera positive by RIA. In addition, two patients with primary biliary cirrhosis and one patient with chronic active hepatitis were AMA positive when tested by indirect immunofluorescence, but negative when tested by RIA. AMAs were not detected by RIA or indirect immunofluorescence in 121 further patients with various hepatic and nonhepatic diseases, including healthy controls. The RIA detects an AMA directed against one determinant of a mitochondrial antigen which bears the characteristics of the previously described primary biliary cirrhosis antigen (M2 antigen). The RIA described is a sensitive and specific test for the detection of one mitochondrial autoantibody associated with chronic cholestatic liver diseases displaying the morphology of primary biliary cirrhosis in chronic active hepatitis or liver biopsy specimens. Antibodies against other mitochondrial antigens do not react in this assay system.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840020102","type":"journal-article","created":{"date-parts":[[2010,7,5]],"date-time":"2010-07-05T13:11:26Z","timestamp":1278335486000},"page":"1S-7S","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":54,"title":["A Mitochondrial Antigen-Antibody System in Cholestatic Liver Disease Detected by Radioimmunoassay"],"prefix":"10.1097","volume":"2","author":[{"given":"Michael","family":"Manns","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl-Hermann","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zum","family":"B\u00fcschenfelde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840020102-BIB1|cit1","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/S0140-6736(65)91372-3","article-title":"Serological tests in diagnosis of primary biliary cirrhosis","volume":"1","author":"Walker","year":"1965","journal-title":"Lancet"},{"key":"10.1002\/hep.1840020102-BIB2|cit2","doi-asserted-by":"crossref","first-page":"533","DOI":"10.7326\/0003-4819-77-4-533","article-title":"Mitochondrial antibody in primary biliary cirrhosis and other diseases","volume":"77","author":"Klatskin","year":"1972","journal-title":"Ann Intern Med"},{"key":"10.1002\/hep.1840020102-BIB3|cit3","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1055\/s-0028-1106763","article-title":"Ein Lupus-erythematodes-ahnliches Syndrom mit antimitochondrialen Antikorpern","volume":"98","author":"Maas","year":"1973","journal-title":"Dtsch Med Wschr"},{"key":"10.1002\/hep.1840020102-BIB4|cit4","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/S0140-6736(75)90055-0","article-title":"Drug-induced pseudolupus","volume":"2","author":"Grob","year":"1975","journal-title":"Lancet"},{"key":"10.1002\/hep.1840020102-BIB5|cit5","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1084\/jem.126.2.277","article-title":"Mitochondrial antibodies in primary biliary cirrhosis. 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Localization of the antigen to mitochondrial membranes","volume":"126","author":"Berg","year":"1967","journal-title":"J Exp Med"},{"key":"10.1002\/hep.1840020102-BIB6|cit6","volume-title":"WHO Manual of Autoimmune Serology","author":"Roitt","year":"1969"},{"key":"10.1002\/hep.1840020102-BIB7|cit7","first-page":"599","article-title":"Precipitating antibodies to mitochondrial antigens in patients with primary biliary cirrhosis","volume":"39","author":"Miyachi","year":"1980","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840020102-BIB8|cit8","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1055\/s-0028-1106345","article-title":"Heterogenitat mitochondrialer Autoantikorper","volume":"100","author":"Berg","year":"1975","journal-title":"Dtsch Med Wschr"},{"key":"10.1002\/hep.1840020102-BIB9|cit9","first-page":"357","article-title":"A new pattern of non-organ- non-species-specific anti-organelle antibody detected by immunofluorescence: the mitochondrial antibody number 5","volume":"31","author":"Labro","year":"1978","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840020102-BIB10|cit10","first-page":"43","article-title":"Mitochondrial antibodies in chronic liver diseases and connective tissue disorders: further characterization of the autoantigens","volume":"41","author":"Meek","year":"1980","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840020102-BIB11|cit11","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1016\/S0140-6736(80)92397-1","article-title":"Serological classification of chronic cholestatic liver disease by the use of two different types of antimitochondrial antibodies","volume":"2","author":"Berg","year":"1980","journal-title":"Lancet"},{"key":"10.1002\/hep.1840020102-BIB12|cit12","first-page":"68","article-title":"Heterogeneity of anti-mitochondrial antibodies; characterization and separation of the antigen associated with the pseudolupus erythematosus syndrome","volume":"37","author":"Sayers","year":"1979","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840020102-BIB13|cit13","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/S0140-6736(68)90710-1","article-title":"A classification of chronic hepatitis","volume":"2","author":"De Groote","year":"1968","journal-title":"Lancet"},{"key":"10.1002\/hep.1840020102-BIB14|cit14","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1056\/NEJM197603112941103","article-title":"Detection of a liver membrane autoantibody in HBsAg negative chronic active hepatitis","volume":"294","author":"Hopf","year":"1976","journal-title":"N Engl J Med"},{"key":"10.1002\/hep.1840020102-BIB15|cit15","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1128\/JVI.12.5.995-1005.1973","article-title":"DNA polymerase associated with human hepatitis B antigen","volume":"12","author":"Kaplan","year":"1973","journal-title":"J Virol"},{"key":"10.1002\/hep.1840020102-BIB16|cit16","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840020102-BIB17|cit17","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/194495a0","article-title":"Preparation of iodine\u2212131 labelled human growth hormone of high specific activity","volume":"194","author":"Hunter","year":"1962","journal-title":"Nature"},{"key":"10.1002\/hep.1840020102-BIB18|cit18","first-page":"263","article-title":"Detection and characterization of liver membrane autoantibodies in chronic active hepatitis by a solid-phase radioimmunoassay","volume":"42","author":"Manns","year":"1980","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840020102-BIB19|cit19","first-page":"311","article-title":"Further purification of the mitochondrial inner membrane autoantigen reacting with primary biliary cirrhosis sera","volume":"26","author":"Ben-Yoseph","year":"1974","journal-title":"Immunology"},{"key":"10.1002\/hep.1840020102-BIB20|cit20","first-page":"517","article-title":"A human-specific mitochondrial antibody. 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