{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:10:49Z","timestamp":1790644249393,"version":"4.1.0"},"reference-count":20,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":11445,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1984,5]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>A solid-phase, noncompetitive radioimmunoassay has been developed for aldolase B in human serum and tissues. Aldolase B was purified from human liver, and specific antisera to purified aldolase B were obtained from chickens. Specific antihuman aldolase B IgG was purified by affinity chromatography. Disposable polypropylene plates were coated with affinity purified specific IgG antibody and used for radioimmunoassay with <jats:sup>125<\/jats:sup>I-specific IgG antibody to aldolase B. The nonspecific binding was minimized by saturating the binding sites of the plates with 2% ovalbumin in 0.1% Tween 20. This radioimmunoassay is specific for the aldolase B subunit, with no cross-reactivity with human aldolase A or aldolase C subunits. Aldolase B is predominantly found in normal liver. Relatively high aldolase B levels are also observed in kidney. Serum levels of aldolase B in 21 normal subjects ranged from 21 to 39 ng per ml, with a mean of 28.7 \u00b1 8.6 (2 S.D.) ng per ml. Forty of 42 (95%) patients with acute and chronic hepatitis without cirrhosis had serum aldolase B levels greater than 40 ng per ml. Serum aldolase B levels correlated well with total serum aldolase enzyme activities (r = 0.967) and SGPT (r = 0.951) in patients with liver diseases. In cancer patients, serum aldolase B was slightly elevated in 15 of 26 (58%) patients with cancer metastatic to the liver or primary liver cell carcinoma, whereas no elevation of serum aldolase B was observed in 16 cancer patients without liver metastasis. Measurements of aldolase B serum levels by radioimmunoassay appear to be a useful measure of liver cell necrosis from benign or malignant liver diseases.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840040329","type":"journal-article","created":{"date-parts":[[2008,8,22]],"date-time":"2008-08-22T13:28:13Z","timestamp":1219411693000},"page":"531-535","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":22,"title":["Human aldolase B serum levels: A marker of liver injury"],"prefix":"10.1097","volume":"4","author":[{"given":"Masahiro","family":"Asaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamotsu","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaine F.","family":"Hollinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elliot","family":"Alpert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840040329-BIB1","doi-asserted-by":"crossref","first-page":"3280","DOI":"10.1016\/S0021-9258(18)48659-0","article-title":"Comparative studies of liver and muscle aldolase","volume":"238","author":"Blostein","year":"1963","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840040329-BIB2","first-page":"1248","article-title":"Evolution of aldolase","volume":"23","author":"Rutter","year":"1964","journal-title":"Feb Proc"},{"key":"10.1002\/hep.1840040329-BIB3","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/j.1749-6632.1968.tb11881.x","article-title":"Aldolase variants: structure and physiological significance","volume":"151","author":"Rutter","year":"1968","journal-title":"Ann Acad Sci"},{"key":"10.1002\/hep.1840040329-BIB4","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1021\/bi00829a016","article-title":"Distribution of fructose diphosphate aldolase variants in biological systems","volume":"8","author":"Lebherz","year":"1969","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840040329-BIB5","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1021\/bi00839a025","article-title":"Isolation of fructose diphosphate aldolase A, B, and C","volume":"8","author":"Penhoet","year":"1969","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840040329-BIB6","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1038\/200995a0","article-title":"Anomaly of aldolase in primary liver cancer","volume":"200","author":"Schapira","year":"1963","journal-title":"Nature"},{"key":"10.1002\/hep.1840040329-BIB7","first-page":"415","article-title":"Changes in aldolase isoenzyme patterns of human cancerous tissues","volume":"59","author":"Tsunematsu","year":"1968","journal-title":"Gann"},{"key":"10.1002\/hep.1840040329-BIB8","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1002\/1097-0142(196909)24:3<637::AID-CNCR2820240332>3.0.CO;2-Z","article-title":"Aldolase isoenzymes in human tissue and serum","volume":"24","author":"Tsunematsu","year":"1969","journal-title":"Cancer"},{"key":"10.1002\/hep.1840040329-BIB9","first-page":"743","article-title":"Irreversible fixation of increase level of muscle type aldolase activity appearing in rat liver in the early stage of hepatocarcinogenesis","volume":"30","author":"Endo","year":"1970","journal-title":"Cancer Res"},{"key":"10.1002\/hep.1840040329-BIB10","first-page":"1224","article-title":"Fetal pattern of aldolase in transplantable hepatomas","volume":"31","author":"Schapira","year":"1971","journal-title":"Cancer Res"},{"key":"10.1002\/hep.1840040329-BIB11","first-page":"31","article-title":"Changes in the activities of glucose-6-phosphatase, aldolase and alkaline phosphatase during azo-dye carciogenesis","volume":"63","author":"Kaneko","year":"1972","journal-title":"Gann"},{"key":"10.1002\/hep.1840040329-BIB12","first-page":"187","article-title":"Human aldolase B subunit-specific radioimmunoassay","volume":"4","author":"Asaka","year":"1983","journal-title":"Oncodevel Biol Med"},{"key":"10.1002\/hep.1840040329-BIB13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840040329-BIB14","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/194495a0","article-title":"Preparation of iodine-131 labeled human growth hormone of high specific activity","volume":"194","author":"Hunter","year":"1962","journal-title":"Nature"},{"key":"10.1002\/hep.1840040329-BIB15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0009-8981(82)90042-0","article-title":"A non-competitive solid-phase radioimmunoassay for human aldolase A","volume":"125","author":"Asaka","year":"1982","journal-title":"Clin Chim Acta"},{"key":"10.1002\/hep.1840040329-BIB16","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1042\/bj1330429","article-title":"A comparative study of aldolase from human muscle and liver","volume":"133","author":"Eagles","year":"1972","journal-title":"Biochem J"},{"key":"10.1002\/hep.1840040329-BIB17","first-page":"433","article-title":"Diagnosis of cancer by radioimmunoassay of muscle type aldolase","volume":"71","author":"Asaka","year":"1980","journal-title":"Gann"},{"key":"10.1002\/hep.1840040329-BIB18","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0009-8981(81)90116-9","article-title":"Radioimmunoassay for human aldolase A","volume":"117","author":"Asaka","year":"1981","journal-title":"Clin Chim Acta"},{"key":"10.1002\/hep.1840040329-BIB19","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0016-5085(83)80180-2","article-title":"Aldolase A isoenzyme levels in serum and tissues of patients with liver diseases","volume":"84","author":"Asaka","year":"1983","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840040329-BIB20","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1002\/1097-0142(19830515)51:10<1873::AID-CNCR2820511020>3.0.CO;2-U","article-title":"Radioimmunoassay of aldolase A: determination of normal serum levels and increased serum concentration in cancer patients","volume":"51","author":"Asaka","year":"1984","journal-title":"Cancer"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840040329","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-198405000-00029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:01:04Z","timestamp":1733050864000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-198405000-00029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,5]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1984]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840040329","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1984,5]]}}}