{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,8]],"date-time":"2025-02-08T05:23:36Z","timestamp":1738992216828,"version":"3.37.0"},"reference-count":7,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":9923,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,7]]},"abstract":"\n \n 16,16 Dimethyl prostaglandin E2<\/jats:sub> (dmPGE2<\/jats:sub>), a known cytoprotective agent, was examined for its ability to alter the course of fulminant hepatitis in an experimental model of fulminant viral hepatitis, murine hepatitis virus type 3 (MHV-3). Fully susceptible BALB\/cJ mice, infected with 100 50% lethal doses (LD50<\/jats:sub>) of MHV-3 developed histologic and biochemical evidence of fulminant hepatitis, as evidenced by massive hepatic necrosis with hypoglycemia, metabolic acidosis, and a markedly elevated serum alanine aminotransferase (ALT) (mean, 1,402 \u00b1 619 IU\/liter). In contrast, animals treated with dmPGE2<\/jats:sub> either before or after infection (up to 48 h) demonstrated a marked reduction in both histologic and biochemical evidence of liver damage as characterized by normal blood glucose, total CO2<\/jats:sub>, and ALT determinations (mean ALT, 63 \u00b1 40 IU\/liter). Treatment of infected mice with PGF2<\/jats:sub> demonstrated no cytoprotective effects. High titers of infectious virus were recovered from the livers of both dmPGE2<\/jats:sub>-treated and -untreated animals throughout the course of infection.<\/jats:p>\n In a parallel in vitro<\/jats:italic> study, dmPGE2<\/jats:sub> (10\u22124<\/jats:sup>-10\u22128<\/jats:sup> M) demonstrated a similar cytoprotective effect on monolayers of isolated cultured hepatocytes from fully susceptible BALB\/cJ mice infected at a multiplicity of infection of 0.1, 1.0, and 10.0. In addition, splenic macrophages recovered from infected and untreated BALB\/cJ mice demonstrated a marked augmentation in procoagulant activity (PCA) from a basal 10 \u00b1 5 mU\/106<\/jats:sup> splenic macrophages to a maximum of 615 \u00b1 102 mU\/106<\/jats:sup> splenic macrophages, whereas no increase in macrophage PCA was detected in infected animals treated with dmPGE2<\/jats:sub>. These results suggest that dmPGE2<\/jats:sub>, without detectably altering viral replication or infectivity in vivo<\/jats:italic>, confers a marked cytoprotective effect on hepatocytes both in vivo<\/jats:italic> and in vitro<\/jats:italic>, and prevents the induction of macrophage PCA in vivo<\/jats:italic> in fully susceptible BALB\/cJ mice after murine hepatitis virus type 3 infection.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/hep.1840080443","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T21:24:46Z","timestamp":1172957086000},"page":"969-970","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Hepatic cytoprotection by prostaglandins: Theories unlimited"],"prefix":"10.1097","volume":"8","author":[{"given":"Robert A.","family":"Levine","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840080443-BIB1","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/0016-5085(79)90235-X","volume":"77","author":"Robert","year":"1979","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840080443-BIB2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0016-5085(81)80049-2","volume":"81","author":"Stachura","year":"1981","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840080443-BIB3","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0090-6980(81)90124-6","volume":"21","author":"Ruwart","year":"1981","journal-title":"Prostaglandins"},{"key":"10.1002\/hep.1840080443-BIB4","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1080\/15287398509530674","volume":"15","author":"Raheja","year":"1985","journal-title":"J Toxicol Environ Health"},{"key":"10.1002\/hep.1840080443-BIB5","first-page":"311","volume":"18","author":"Stachura","year":"1980","journal-title":"Folia Histochem Cytochem"},{"key":"10.1002\/hep.1840080443-BIB6","first-page":"1104","volume":"7","author":"Abecassis","year":"1987","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840080443-BIB7","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1002\/hep.1840070603","volume":"7","author":"Mizoguchi","year":"1987","journal-title":"Hepatology"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840080443","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-198807000-00042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T15:11:27Z","timestamp":1738941087000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-198807000-00042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1988]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840080443","relation":{},"ISSN":["0270-9139"],"issn-type":[{"type":"print","value":"0270-9139"}],"subject":[],"published":{"date-parts":[[1988,7]]}}}