{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T06:34:28Z","timestamp":1770532468627,"version":"3.49.0"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":9680,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,3]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Fat-storing cells were isolated from 15-day-old mouse sinusoidal cell cultures (Kupffer or endothelial cells), where they had multiplied abundantly; they were then purified by a negative selection method based on the fact that they do not possess Fc receptors, as do both other types of cells.<\/jats:p>\n            <jats:p>The fat-storing cells, which could be subcultured for at least 10 passages, have the main morphological characteristics already described <jats:italic toggle=\"yes\">in vivo<\/jats:italic>, in particular, the rough endoplasmic reticulum and the lipid droplets, which become less and less apparent as the number of passages increases. Subcultured fat-storing cells, almost devoid of lipid droplets and vitamin A, were able to take up retinol, as the appearance of a typical autofluorescence indicated; the number of lipid droplets increased concomitantly. Furthermore, the cultured fat-storing cells were able to internalize one-\u03bcm-sized latex beads by phagocytosis. Infection of fat-storing cells with mouse hepatitis virus 3, ectromelia or Sindbis virus led to multiplication of the virus particles. There was a direct relation between the multiplication of mouse hepatitis virus 3 in cultured fat-storing cells and the susceptibility of the animals to the virus. In the case of Sindbis virus, interferon is produced, its production being independent of the presence of vitamin A.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840090303","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T22:56:32Z","timestamp":1172962592000},"page":"352-362","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":27,"title":["Isolation, culture and main characteristics of mouse fat-storing cells: Interaction with viruses"],"prefix":"10.1097","volume":"9","author":[{"given":"Wan","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Louis","family":"Gendrault","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne-Marie","family":"Steffan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Jeandidier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Kirn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840090303-BIB1","first-page":"377","volume-title":"Progress in liver diseases","author":"Kirn","year":"1982","unstructured":", . Interaction of viruses with sinusoidal cells. In: , eds. Progress in liver diseases, Vol. VII. New York: Grune & Stratton, 1982: 377\u2013392."},{"key":"10.1002\/hep.1840090303-BIB2","first-page":"99","volume":"132","author":"Reinke","year":"1986","journal-title":"Zentralbl Allg Pathol"},{"key":"10.1002\/hep.1840090303-BIB3","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1099\/0022-1317-65-9-1617","volume":"65","author":"Pereira","year":"1984","journal-title":"J Gen Virol"},{"key":"10.1002\/hep.1840090303-BIB4","first-page":"377","volume-title":"Cells of the hepatic sinusoid","volume":"1","author":"Steffan","year":"1986","unstructured":", . Role of sinusoidal cells in the course of the hepatitis induced by mouse hepatitis virus type 3 (MHV 3) in mice. In: , , eds. Cells of the hepatic sinusoid, Vol. 1. Rijswijk, The Netherlands: The Kupffer Cell Foundation, 1986: 377\u2013378."},{"key":"10.1002\/hep.1840090303-BIB5","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1247\/csf.10.361","volume":"10","author":"Tsuchiya","year":"1985","journal-title":"Cell Struct Funct"},{"key":"10.1002\/hep.1840090303-BIB6","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1177\/28.11.7000887","volume":"28","author":"Grimaud","year":"1980","journal-title":"J Histochem Cytochem"},{"key":"10.1002\/hep.1840090303-BIB7","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1002\/hep.1840060505","volume":"6","author":"Steffan","year":"1986","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB8","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/0014-4827(85)90243-5","volume":"160","author":"Hendriks","year":"1985","journal-title":"Exp Cell Res"},{"key":"10.1002\/hep.1840090303-BIB9","doi-asserted-by":"crossref","first-page":"382","DOI":"10.3181\/00379727-180-42193","volume":"180","author":"Leo","year":"1985","journal-title":"Proc Soc Exp Biol Med"},{"key":"10.1002\/hep.1840090303-BIB10","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1099\/0022-1317-66-3-509","volume":"66","author":"G\u00f6ncz\u00f6l","year":"1985","journal-title":"J Gen Virol"},{"key":"10.1002\/hep.1840090303-BIB11","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-7091-8615-2","volume-title":"The interferon system","author":"Stewart","year":"1981","unstructured":"The interferon system, ed. 2. Vienna: Springer-Verlag, 1981."},{"key":"10.1002\/hep.1840090303-BIB12","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1002\/hep.1840070630","volume":"7","author":"Hendriks","year":"1987","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB13","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/0014-4827(82)90283-X","volume":"139","author":"Knook","year":"1982","journal-title":"Exp Cell Res"},{"key":"10.1002\/hep.1840090303-BIB14","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1002\/hep.1840040307","volume":"4","author":"De Leeuw","year":"1984","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB15","doi-asserted-by":"crossref","first-page":"451","DOI":"10.2220\/biomedres.5.451","volume":"5","author":"Senoo","year":"1984","journal-title":"Biomed Res"},{"key":"10.1002\/hep.1840090303-BIB16","doi-asserted-by":"crossref","first-page":"480","DOI":"10.2957\/kanzo.27.480","volume":"27","author":"Matsuura","year":"1986","journal-title":"Acta Hepatol Jap"},{"key":"10.1002\/hep.1840090303-BIB17","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/S0168-8278(86)80481-0","volume":"3","author":"Shiratori","year":"1986","journal-title":"J Hepatol"},{"key":"10.1002\/hep.1840090303-BIB18","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1111\/j.1600-0676.1986.tb01072.x","volume":"6","author":"Shiratori","year":"1986","journal-title":"Liver"},{"key":"10.1002\/hep.1840090303-BIB19","first-page":"473","volume-title":"Cells of the hepatic sinusoid","volume":"1","author":"Tanaka","year":"1986","unstructured":", , et al. Isolation and higher purification of fat-storing cells from rat liver with flow cytometry. In: , , eds. Cells of the hepatic sinusoid, Vol. 1. Rijswijk, The Netherlands: The Kupffer Cell Foundation, 1986: 473\u2013474."},{"key":"10.1002\/hep.1840090303-BIB20","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/j.1432-1033.1987.tb11058.x","volume":"164","author":"Blaner","year":"1987","journal-title":"Eur J Biochem"},{"key":"10.1002\/hep.1840090303-BIB21","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/S0168-8278(87)80079-X","volume":"4","author":"Ramadori","year":"1987","journal-title":"J Hepatol"},{"key":"10.1002\/hep.1840090303-BIB22","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1002\/hep.1840070411","volume":"7","author":"Sch\u00e4fer","year":"1987","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB23","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1002\/hep.1840070212","volume":"7","author":"Tsutsumi","year":"1987","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB24","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1002\/hep.1840070609","volume":"7","author":"Yamada","year":"1987","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB25","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/0014-4827(84)90713-4","volume":"150","author":"Blomhoff","year":"1984","journal-title":"Exp Cell Res"},{"key":"10.1002\/hep.1840090303-BIB26","doi-asserted-by":"crossref","first-page":"8681","DOI":"10.1073\/pnas.82.24.8681","volume":"82","author":"Friedman","year":"1985","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1002\/hep.1840090303-BIB27","first-page":"183","volume":"54","author":"Kawase","year":"1986","journal-title":"Exp Cell Biol"},{"key":"10.1002\/hep.1840090303-BIB28","doi-asserted-by":"crossref","first-page":"10280","DOI":"10.1016\/S0021-9258(18)61109-3","volume":"262","author":"Davis","year":"1987","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840090303-BIB29","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0003-2697(87)90673-7","volume":"161","author":"Friedman","year":"1987","journal-title":"Anal Biochem"},{"key":"10.1002\/hep.1840090303-BIB30","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/0016-5085(88)90625-7","volume":"94","author":"Yamauchi","year":"1988","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840090303-BIB31","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S0074-7696(08)61977-4","volume":"66","author":"Wake","year":"1980","journal-title":"Int Rev Cytol"},{"key":"10.1002\/hep.1840090303-BIB32","first-page":"495","volume-title":"The liver: biology and pathobiology","author":"Fahimi","year":"1982","unstructured":"Sinusoidal endothelial cells and perisinusoidal fat-storing cells: structure and function. In; , , et al., eds. The liver: biology and pathobiology. New York: Raven Press, 1982: 495\u2013506."},{"key":"10.1002\/hep.1840090303-BIB33","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1002\/hep.1840040425","volume":"4","author":"Yokoi","year":"1984","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB34","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1002\/path.1711500106","volume":"105","author":"Burt","year":"1986","journal-title":"J Pathol"},{"key":"10.1002\/hep.1840090303-BIB35","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1267\/ahc.19.219","volume":"19","author":"Miyazaki","year":"1986","journal-title":"Acta Histochem Cytochem"},{"key":"10.1002\/hep.1840090303-BIB36","first-page":"782","volume":"6","author":"Ogiwara","year":"1986","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB37","first-page":"88","volume":"58","author":"Tsutsumi","year":"1988","journal-title":"Lab Invest"},{"key":"10.1002\/hep.1840090303-BIB38","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/0016-5085(88)90566-5","volume":"94","author":"Maher","year":"1988","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840090303-BIB39","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1002\/hep.1840060212","volume":"6","author":"Cl\u00e9ment","year":"1986","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB40","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1007\/BF01296379","volume":"32","author":"Shiratori","year":"1987","journal-title":"Dig Dis Sci"},{"key":"10.1002\/hep.1840090303-BIB41","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S0769-2617(82)80071-3","volume":"133E","author":"Steffan","year":"1982","journal-title":"Ann Virol (Inst Pasteur)"},{"key":"10.1002\/hep.1840090303-BIB42","doi-asserted-by":"crossref","first-page":"640","DOI":"10.4049\/jimmunol.115.3.640","volume":"115","author":"Le Pr\u00e9vost","year":"1975","journal-title":"J Immunol"},{"key":"10.1002\/hep.1840090303-BIB43","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/0168-1702(84)90013-3","volume":"1","author":"Pereira","year":"1984","journal-title":"Virus Res"},{"key":"10.1002\/hep.1840090303-BIB44","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1128\/JVI.55.3.890-891.1985","volume":"55","author":"Wallace","year":"1985","journal-title":"J Virol"},{"key":"10.1002\/hep.1840090303-BIB45","first-page":"676","volume":"2","author":"Kirn","year":"1982","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840090303-BIB46","first-page":"413","volume-title":"Sinusoidal liver cells","author":"Kirn","year":"1982","unstructured":", , et al. Interaction between viruses and primary cultures of murine and human liver endothelial cells. In: , eds. Sinusoidal liver cells. Amsterdam, The Netherlands: Elsevier Biomedical Press, 1982: 413\u2013420."},{"key":"10.1002\/hep.1840090303-BIB47","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0162-3109(82)90051-0","volume":"4","author":"Abb","year":"1982","journal-title":"Immunopharmacology"},{"key":"10.1002\/hep.1840090303-BIB48","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1099\/0022-1317-32-1-143","volume":"32","author":"Blalock","year":"1976","journal-title":"J Gen Virol"},{"key":"10.1002\/hep.1840090303-BIB49","doi-asserted-by":"crossref","first-page":"5382","DOI":"10.1073\/pnas.74.12.5382","volume":"74","author":"Blalock","year":"1977","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1002\/hep.1840090303-BIB50","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1099\/0022-1317-29-3-315","volume":"29","author":"Blalock","year":"1975","journal-title":"J Gen Virol"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840090303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-198903000-00002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T15:14:19Z","timestamp":1738941259000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-198903000-00002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,3]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840090303","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1989,3]]}}}