{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:17:45Z","timestamp":1790533065092,"version":"4.1.0"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":9254,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,5]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Antibodies against cytochrome P\u2013450 are found in some children with autoimmune hepatitis (antiliver\/kidney microsome) and in patients with ticrynafen hepatitis (antiliver\/kidney microsome<jats:sub>2<\/jats:sub>). For an immune reaction against cytochrome P\u2013450 to possibly destroy the hepatocytes, one must assume that cytochrome P\u2013450 is present on the plasma membrane surface of hepatocytes. In a first series of experiments, plasma membranes were prepared with a technique based on the electrostatic attachment of isolated hepatocytes to polyethyleneimine\u2013coated beads. After vortexing, beads were coated with a very pure plasma membrane fraction. Microsomal contamination, judged from the specific activities of glucose\u20136\u2013phosphatase or NADH\u2013cytochrome <jats:italic toggle=\"yes\">c<\/jats:italic> reductase, was less than 1%. Nevertheless, the specific content (permilligram of protein) of CO\u2013binding cytochrome P\u2013450 was 20% of that in microsomes; the specific benzo (a)pyrene hydroxylase activity was 25%, and ethoxycoumarin deethylase 11%, Immunoblots showed the presence of cytochromes P\u2013450 UT\u2013A, UT\u2013H, PB\u2013B, ISF\u2013G and PCN\u2013E, the last three isoenzymes being inducible by, respectively, phenobarbital, 3\u2013methylcholanthrene and dexamethasone. In a second series of experiments, nonpermeabilized isolated hepatocytes from untreated rats were incubated with anticytochrome P\u2013450 antibodies. Immunfluorescence and immunoperoxidase staining confirmed the presence of cytochromes P\u2013450 UT\u2013A, PB\u2013B and ISF\u2013G on the membrane. In a last series of experiments, human antiliver kidney microsomal<jats:sub>1<\/jats:sub> antibodies were found to react specifically with rat liver plasma membrane cytochrome P\u2013450 UT\u2013H (IID subfamily). We conclude that several cytochrome P\u2013450 isoenzymes are present, active and inducible on the plasma membrane surface of hepatocytes. It is therefore conceivable that immunization against plasma membrane cytochrome P\u2013450 might lead to the immunological destruction of hepatocytes in some patients.(HEPATOLGOY 1990; 11: 850\u2013858.)<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840110521","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:27:36Z","timestamp":1172964456000},"page":"850-858","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":86,"title":["Presence of Functional Cytochrome P\u2013450 on Isolated Rat Hepatocyte Plasma Membrane"],"prefix":"10.1097","volume":"11","author":[{"given":"Jacqueline","family":"Loeper","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronique","family":"Descatoire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Maurice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Beaune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Feldmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Larrey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Pessayre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840110521-BIB1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/dna.1.1989.8.1","volume":"8","author":"Nebert","year":"1989","journal-title":"DNA"},{"key":"10.1002\/hep.1840110521-BIB2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.3109\/10408448509037460","volume":"14","author":"Guengerich","year":"1985","journal-title":"CRC 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