{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,8]],"date-time":"2025-02-08T05:25:08Z","timestamp":1738992308508,"version":"3.37.0"},"reference-count":21,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":9162,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>We have studied antibodies (anti\u2013pol antibody) against the polymerase gene product of hepatitis B virus by solid\u2013phase enzyme immunoassay using synthetic peptides coded for by this gene. Sera from six patients with acute hepatitis B, 112 chronic hepatitis B virus carriers and six healthy individuals with naturally acquired immunity to hepatitis B virus were tested for anti\u2013pol antibody. In acute hepatitis B virus infection, anti\u2013pol antibody was detected in three of six patients. In chronic hepatitis B virus infection, antipol antibody was detected in 17 of 29 (59%), in 23 of 33 (70%) of cirrhotic patients and in 18 of 24 (75%) patients with cirrhosis complicated by hepatocellular carcinoma, compared with 4 of 19 (21%) asymptomatic carriers and 2 of 7 (29%) patients with chronic persistent hepatitis. Titers of anti\u2013pol antibody were higher in cirrhotic patients with and without hepatocellular carcinoma than in patients with chronic active hepatitis. The presence of anti\u2013pol antibody, however, had no relationship with hepatitis B virus\u2013associated DNA polymerase activities and other viral replicative markers. As for sera from six healthy individuals with naturally acquired immunity to hepatitis B virus, two (33%) were positive for anti\u2013pol antibody. These results indicate that the immune response toward the polymerase gene product is induced during acute and chronic hepatitis B virus infection. In chronic hepatitis B virus infection, anti\u2013pol antibody may serve as a new marker indicative of a long period of hepatitis B virus\u2013induced hepatitis. (Hepatology 1990;12:193-198).<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840120203","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:35:38Z","timestamp":1172964938000},"page":"193-198","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":12,"title":["Detection of Antibodies Against the Polymerase Gene Product in Hepatitis B Virus Infection"],"prefix":"10.1097","volume":"12","author":[{"given":"Nobukazu","family":"Yuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akinori","family":"Kasahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Katayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyuki","family":"Fusamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takenobu","family":"Kamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840120203-BIB1","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/281646a0","volume":"281","author":"Galibert","year":"1979","journal-title":"Nature"},{"key":"10.1002\/hep.1840120203-BIB2","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1093\/infdis\/136.5.617","volume":"136","author":"Cappel","year":"1977","journal-title":"J Infect Dis"},{"key":"10.1002\/hep.1840120203-BIB3","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1002\/jmv.1890240202","volume":"24","author":"Feitelson","year":"1988","journal-title":"J Med Virol"},{"key":"10.1002\/hep.1840120203-BIB4","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1099\/0022-1317-69-3-689","volume":"69","author":"Stemler","year":"1988","journal-title":"J Gen Virol"},{"key":"10.1002\/hep.1840120203-BIB5","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1002\/hep.1840100314","volume":"10","author":"Chang","year":"1989","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840120203-BIB6","doi-asserted-by":"crossref","first-page":"3750","DOI":"10.4049\/jimmunol.143.11.3750","volume":"143","author":"Weimer","year":"1989","journal-title":"J Immunol"},{"key":"10.1002\/hep.1840120203-BIB7","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/0092-8674(82)90157-X","volume":"29","author":"Summers","year":"1982","journal-title":"Cell"},{"key":"10.1002\/hep.1840120203-BIB8","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/0042-6822(84)90330-1","volume":"139","author":"Miller","year":"1984","journal-title":"Virology"},{"key":"10.1002\/hep.1840120203-BIB9","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1126\/science.3961490","volume":"232","author":"Seeger","year":"1986","journal-title":"Science"},{"key":"10.1002\/hep.1840120203-BIB10","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/305827a0","volume":"305","author":"Toh","year":"1983","journal-title":"Nature"},{"key":"10.1002\/hep.1840120203-BIB11","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1128\/JVI.62.2.626-628.1988","volume":"62","author":"Bavand","year":"1988","journal-title":"J Virol"},{"key":"10.1002\/hep.1840120203-BIB12","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1128\/JVI.63.2.1019-1021.1989","volume":"63","author":"Bavand","year":"1989","journal-title":"J Virol"},{"key":"10.1002\/hep.1840120203-BIB13","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1126\/science.2418504","volume":"231","author":"Veronese","year":"1986","journal-title":"Science"},{"key":"10.1002\/hep.1840120203-BIB14","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1126\/science.2435004","volume":"235","author":"Laurence","year":"1987","journal-title":"Science"},{"key":"10.1002\/hep.1840120203-BIB15","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/BF00915727","volume":"7","author":"Chatterjee","year":"1987","journal-title":"J Clin Immunol"},{"key":"10.1002\/hep.1840120203-BIB16","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1128\/JVI.12.5.995-1005.1973","volume":"12","author":"Kaplan","year":"1973","journal-title":"J Virol"},{"key":"10.1002\/hep.1840120203-BIB17","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0378-1119(84)90124-0","volume":"30","author":"Kobayashi","year":"1984","journal-title":"Gene"},{"key":"10.1002\/hep.1840120203-BIB18","doi-asserted-by":"crossref","first-page":"4786","DOI":"10.1128\/JVI.62.12.4786-4790.1988","volume":"62","author":"Mack","year":"1988","journal-title":"J Virol"},{"key":"10.1002\/hep.1840120203-BIB19","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1126\/science.2418501","volume":"231","author":"Will","year":"1986","journal-title":"Science"},{"key":"10.1002\/hep.1840120203-BIB20","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0042-6822(88)90151-1","volume":"166","author":"Raimondo","year":"1988","journal-title":"Virology"},{"key":"10.1002\/hep.1840120203-BIB21","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0168-8278(89)90165-7","volume":"8","author":"Theilmann","year":"1989","journal-title":"J Hepatol"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840120203","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199008000-00002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T15:37:28Z","timestamp":1738942648000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199008000-00002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840120203","relation":{},"ISSN":["0270-9139"],"issn-type":[{"type":"print","value":"0270-9139"}],"subject":[],"published":{"date-parts":[[1990,8]]}}}