{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,8]],"date-time":"2025-02-08T05:24:27Z","timestamp":1738992267669,"version":"3.37.0"},"reference-count":20,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":9162,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>The plasma disappearance rate of sulfobromophthalein (V<jats:sub>BSP<\/jats:sub>; \u03bcmol\/kg\/min) was measured in 15 Gilbert's syndrome patients and 12 control subjects after intravenous injection of two different doses (0.59 and 5.90 \u03bcmol\/kg) of the dye. Plasma disappearance rate was significantly reduced in Gilbert's syndrome patients after administration of 0.59 \u03bcmol sulfobromophthalein\/kg (0.119 \u00b1 0.016 vs. 0.146 \u00b1 0.018 \u03bcmol\/kg\/min; mean \u00b1 S.D.; p &lt; 0.001), whereas no difference was found with the higher dose (0.754 \u00b1 0.040 vs. 0.767 \u00b1 0.072 \u03bcmol\/kg\/min). Significant reduction was also found after administration to four Gilbert's syndrome patients and four control subjects of 0.29 and 2.95 \u03bcmol sulfobromophthalein (0.060 \u00b1 0.005 \u03bcmol\/kg\/min vs. 0.077 \u00b1 0.07 \u03bcmol\/kg\/min and 0.480 \u00b1 0.012 \u03bcmol\/kg\/min vs. 0.591 \u00b1 0.015 \u03bcmol\/kg\/min, respectively; p &lt; 0.01). Competition studies with combined administration of sulfobromophthalein (0.59 \u03bcmol\/kg) and different doses of rifamycin SV (0.59, 1.47 and 2.95 \u03bcmol\/kg) showed a significant (p &lt; 0.001) reduction in plasma disappearance rate in Gilbert's syndrome patients but not in controls. The rifamycin SV dose at which a 50% inhibition in plasma disappearance rate was observed was 0.8 \u03bcmol\/kg. The apparent affinity (K<jats:sub>m<\/jats:sub>) of the hepatic transport was higher in Gilbert's syndrome patients than in control subjects (3.61 \u00b1 0.37 \u03bcmol sulfobromophthalein\/kg vs. 2.76 \u00b1 0.29 \u03bcmol sulfobromophthalein\/kg, mean \u00b1 S.D.; p &lt; 0.01), whereas no difference was found in V<jats:sub>max<\/jats:sub> (0.95 \u00b1 0.11 \u03bcmol sulfobromophthalein\/kg vs. 0.93 \u00b1 0.10 \u03bcmol sulfobromophthalein\/kg\/min, mean \u00b1 S.D.; N.S.). We conclude that a defective sulfobromophthalein hepatic transport is present in Gilbert's syndrome, and it may be revealed by lowering the does of the dye. The data sugest that the defect in Gilbert's syndrome is an impaired affinity for sulfobromophthalein of one or more of the putative transport proteins in the basolateral plasma membrane of the hepatocyte. (Hepatology 1990;12:213-217).<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840120206","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:35:38Z","timestamp":1172964938000},"page":"213-217","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":16,"title":["Abnormal Hepatic Uptake of Low Doses of Sulfobromophthalein in Gilbert's Syndrome: the Role of Reduced Affinity of the Plasma Membrane Carrier of Organic Anions"],"prefix":"10.1097","volume":"12","author":[{"given":"Sandro","family":"Gentile","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcello","family":"Persico","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Tiribelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840120206-BIB1","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1136\/jmg.12.2.152","volume":"12","author":"Owens","year":"1975","journal-title":"J Med Genet"},{"key":"10.1002\/hep.1840120206-BIB2","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1056\/NEJM196906052802303","volume":"280","author":"Black","year":"1969","journal-title":"N Engl J Med"},{"key":"10.1002\/hep.1840120206-BIB3","first-page":"245","volume":"27","author":"Billing","year":"1964","journal-title":"Clin Sci"},{"key":"10.1002\/hep.1840120206-BIB4","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/S0002-9343(70)80020-1","volume":"49","author":"Berk","year":"1970","journal-title":"Am J Med"},{"key":"10.1002\/hep.1840120206-BIB5","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S0016-5085(76)80151-5","volume":"70","author":"Martin","year":"1976","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840120206-BIB6","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/S0016-5085(19)33296-2","volume":"63","author":"Berk","year":"1972","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840120206-BIB7","first-page":"166","volume":"107","author":"Gentile","year":"1986","journal-title":"J Lab Clin Med"},{"key":"10.1002\/hep.1840120206-BIB8","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1042\/cs0680675","volume":"68","author":"Gentile","year":"1985","journal-title":"Clin Sci"},{"key":"10.1002\/hep.1840120206-BIB9","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0168-8278(85)80779-0","volume":"1","author":"Gentile","year":"1985","journal-title":"J Hepatol"},{"key":"10.1002\/hep.1840120206-BIB10","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0005-2736(82)90087-6","volume":"685","author":"Lunazzi","year":"1982","journal-title":"Biochim Biophys Acta"},{"key":"10.1002\/hep.1840120206-BIB11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0005-2736(86)90002-7","volume":"856","author":"Baldini","year":"1986","journal-title":"Biochim Biophys Acta"},{"key":"10.1002\/hep.1840120206-BIB12","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/0010-4809(75)90024-5","volume":"8","author":"Cobelli","year":"1975","journal-title":"Comp Biomed Res"},{"key":"10.1002\/hep.1840120206-BIB13","first-page":"539","volume":"54","author":"Okolicsanyi","year":"1978","journal-title":"Clin Sci Mol Med"},{"key":"10.1002\/hep.1840120206-BIB14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/cs0710001","volume":"70","author":"Tiribelli","year":"1986","journal-title":"Clin Sci"},{"key":"10.1002\/hep.1840120206-BIB15","first-page":"50","volume-title":"Methods in enzymology","volume":"174","author":"Sottocasa","year":"1989","unstructured":", . Isolation of bilitranslocase, the transporter of liver plasma membrane for bilirubin and other organic anions. In: , eds. Methods in enzymology. Vol. 174. 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