{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T05:54:55Z","timestamp":1770270895993,"version":"3.49.0"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":9162,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Sera from 81 healthy family members (including husbands) of 13 patients with primary biliary cirrhosis were tested by Western blotting against the antigen fractions M9 and M2 derived respectively from rat liver and beef heart mitochondria. Fifty\u2013eight (70%) were positive and recognized four major (molecular weights 98 kD, 65 kD, 61 kD, 58 kD) and eight minor determinants (85 kD, 81 kD, 78 kD, 54 kD, 48 kD, 45 kD, 40 kD, 30 kD) labeled \u03b1\u2013my. Each of the 58 sera recognized at least one of the four major polypeptides. In contrast, only 6% of 80 primary biliary cirrhosis patients had antibodies against one of these epitopes. Sera from 25 patients with different infectious disorders previously shown to react with submitochondrial particles by enzyme\u2013linked immunosorbent assay also recognized at least one of the four major determinants.<\/jats:p>\n            <jats:p>From these findings it was concluded that these antibodies may belong to the family of natural autoantibodies. Since they reacted with submitochondrial fractions, they were defined as \u201cnaturally occurring mitochondrial antibodies.\u201d<\/jats:p>\n            <jats:p>The high incidence of \u201cnaturally occurring mitochondrial antibodies\u201d in primary biliary cirrhosis contact persons may be taken as indirect evidence for a contagious immunogenic agent circulating in the blood of primary biliary cirrhosis-patients. In contrast, the absence of \u201cnaturally occurring mitochondrial antibodies\u201d in primary biliary cirrhosis-patients themselves implies that an underlying B\u2013cell defect is responsible for this lack of antibody production. Considering the protective role of naturally occurring antibodies in general, this postulated B\u2013cell defect could be a major factor in the etiopathogenesis of primary biliary cirrhosis. (Hepatology 1990; 12:335-341).<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840120222","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:35:38Z","timestamp":1172964938000},"page":"335-341","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":25,"title":["Demonstration of \u201cNaturally Occurring Mitochondrial Antibodies\u201d in Family Members of Patients With Primary Biliary Cirrhosis"],"prefix":"10.1097","volume":"12","author":[{"given":"Reinhild","family":"Klein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter A.","family":"Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840120222-BIB1","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1002\/hep.1840040438","volume":"4","author":"Klein","year":"1984","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840120222-BIB2","first-page":"68","volume":"74","author":"Klein","year":"1988","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840120222-BIB3","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0168-8278(88)80046-1","volume":"6","author":"Klein","year":"1988","journal-title":"J Hepatol"},{"key":"10.1002\/hep.1840120222-BIB4","first-page":"1418","volume":"8","author":"Klein","year":"1988","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840120222-BIB5","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1055\/s-2008-1040501","volume":"9","author":"Berg","year":"1989","journal-title":"Semin Liver 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Anti-M9 antibodies in sera from patients with primary biliary cirrhosis recognize an epitope of glycogen phosphorylase. 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