{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T05:02:31Z","timestamp":1733115751686,"version":"3.30.0"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":8950,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>To test further the competence of the cirrhotic liver to metabolize vitamin D<jats:sub>3<\/jats:sub> at C-25, hepatocytes were isolated from controls and from CCl<jats:sub>4<\/jats:sub>-induced cirrhotic rat livers, as well as from partially hepatectomized rats. The transformation of D<jats:sub>3<\/jats:sub> into 25-hydroxyvitamin D<jats:sub>3<\/jats:sub> was studied in the presence of 10<jats:sup>7<\/jats:sup> hepatocytes at D<jats:sub>3<\/jats:sub> concentrations of 20 nmol\/L to 15.4 \u03bcmol\/L. Histologically, micronodular cirrhosis was present in all CCl<jats:sub>4<\/jats:sub>-treated rats, whereas controls had normal livers; portal venous pressure (p &lt; 0.008) and intrahepatic collagen content (p &lt; 0.0001) were significantly increased in CCl<jats:sub>4<\/jats:sub>-treated rats, whereas no difference was found between the two groups in the total and ionized serum calcium, D<jats:sub>3<\/jats:sub> metabolites, ALT, AST and alkaline phosphatase. Cytochrome P-450 was 0.27 \u00b1 0.02 and 0.25 \u00b1 0.02 nmol\/10<jats:sup>6<\/jats:sup> hepatocytes in controls and cirrhotic rats (N.S.), and it significantly increased in both groups after phenobarbital or 3-methylcholanthrene administration (p &lt; 0.0001). 25-Hydroxyvitamin D<jats:sub>3<\/jats:sub> formation was best described by power law equations and varied between 0.02 \u00b1 0.0004 and 29.57 \u00b1 2.8 in controls, and 0.024 \u00b1 0.0004 and 32.0 \u00b1 7.0 pmol \u00b1 hr<jats:sup>\u22121<\/jats:sup> \u00b1 10<jats:sup>6<\/jats:sup> hepatocytes<jats:sup>\u22121<\/jats:sup> in cirrhotic rats. No statistically significant difference was found in the slopes of the 25-hydroxyvitamin D<jats:sub>3<\/jats:sub> formation, but the y-axis intercept was found to be lower in cirrhotic rats under basal resting conditions (p &lt; 0.005). Inducers of the mixed function oxidases significantly increased 25-hydroxyvitamin D<jats:sub>3<\/jats:sub> formation in controls as well as in cirrhotic rats (p &lt; 0.005). Moreover, both groups were found to respond similarly to the addition of modulators of the enzyme such as the calcium ionophore A23187 and parathyroid hormone. Partial hepatectomy was also without effect on the activation of D<jats:sub>3<\/jats:sub>. Furthermore, the cell sequestration of D<jats:sub>3<\/jats:sub> was also found to be unperturbed in hepatocytes obtained from either cirrhotic or partially hepatectomized livers. The data indicate that in well-compensated micronodular cirrhosis, the C-25 hydroxylation of D<jats:sub>3<\/jats:sub> is generally intrinsically normal at the cellular level and that it also remains fully responsive to <jats:italic toggle=\"yes\">in vivo<\/jats:italic> and <jats:italic toggle=\"yes\">in vitro<\/jats:italic> modulators of its activity. (Hepatology 1991;13:489-499.)<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840130317","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:51:54Z","timestamp":1172965914000},"page":"489-499","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["In micronodular cirrhosis, hepatocytes retain a normal C-25 hydroxylation capacity toward vitamin D3: A study using the rat carbon tetrachloride-induced cirrhotic model"],"prefix":"10.1097","volume":"13","author":[{"given":"Catherine","family":"Dub\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Valli\u00e8res","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"\u00c9thier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nawel","family":"Benbrahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantale","family":"Tremblay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marielle","family":"Gascon-Barr\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840130317-BIB1","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1172\/JCI108389","volume":"57","author":"Olson","year":"1976","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840130317-BIB2","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/j.0954-6820.1977.tb16815.x","volume":"202","author":"Lund","year":"1977","journal-title":"Acta Med Scand"},{"key":"10.1002\/hep.1840130317-BIB3","first-page":"256","volume":"24","author":"Sonnenberg","year":"1977","journal-title":"Acta Hepato-Gastroenterol"},{"key":"10.1002\/hep.1840130317-BIB4","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/BF01464758","volume":"7","author":"Hepner","year":"1976","journal-title":"Dig Dis"},{"key":"10.1002\/hep.1840130317-BIB5","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/S0140-6736(76)92463-6","volume":"2","author":"Long","year":"1976","journal-title":"Lancet"},{"key":"10.1002\/hep.1840130317-BIB6","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1042\/cs0690561","volume":"69","author":"Mawer","year":"1985","journal-title":"Clin Sci"},{"key":"10.1002\/hep.1840130317-BIB7","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1136\/gut.20.10.840","volume":"20","author":"Jung","year":"1979","journal-title":"Gut"},{"key":"10.1002\/hep.1840130317-BIB8","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1172\/JCI105327","volume":"45","author":"Thompson","year":"1966","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840130317-BIB9","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/S0140-6736(77)92660-5","volume":"2","author":"Krawitt","year":"1977","journal-title":"Lancet"},{"key":"10.1002\/hep.1840130317-BIB10","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/0009-8981(78)90179-1","volume":"87","author":"Barragry","year":"1978","journal-title":"Clin Chim Acta"},{"key":"10.1002\/hep.1840130317-BIB11","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1136\/gut.20.7.559","volume":"20","author":"Barragry","year":"1979","journal-title":"Gut"},{"key":"10.1002\/hep.1840130317-BIB12","doi-asserted-by":"crossref","first-page":"349","DOI":"10.3109\/00365528209182066","volume":"17","author":"Danielson","year":"1982","journal-title":"Scand J Gastroenterol"},{"key":"10.1002\/hep.1840130317-BIB13","first-page":"6","volume":"29","author":"Danielson","year":"1982","journal-title":"Hepato-Gastroenterology"},{"key":"10.1002\/hep.1840130317-BIB14","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1002\/hep.1840040215","volume":"4","author":"Bengoa","year":"1984","journal-title":"HEPATOLOGY"},{"key":"10.1002\/hep.1840130317-BIB15","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1002\/hep.1840040216","volume":"2","author":"Mobarhan","year":"1984","journal-title":"HEPATOLOGY"},{"key":"10.1002\/hep.1840130317-BIB16","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0009-8981(78)90309-1","volume":"85","author":"Long","year":"1978","journal-title":"Clin Chim Acta"},{"key":"10.1002\/hep.1840130317-BIB17","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1365-2265.1981.tb00677.x","volume":"15","author":"Dibble","year":"1981","journal-title":"Clin Endocrinol"},{"key":"10.1002\/hep.1840130317-BIB18","first-page":"89","volume":"201","author":"Dibble","year":"1982","journal-title":"Q J Med"},{"key":"10.1002\/hep.1840130317-BIB19","doi-asserted-by":"crossref","first-page":"42","DOI":"10.7326\/0003-4819-103-1-42","volume":"103","author":"Bikle","year":"1985","journal-title":"Ann Intern Med"},{"key":"10.1002\/hep.1840130317-BIB20","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0016-5085(89)90783-X","volume":"96","author":"Diamond","year":"1989","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840130317-BIB21","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1136\/gut.31.1.82","volume":"31","author":"Diamond","year":"1990","journal-title":"Gut"},{"key":"10.1002\/hep.1840130317-BIB22","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1042\/bj1840491","volume":"184","author":"Madhok","year":"1979","journal-title":"Biochem J"},{"key":"10.1002\/hep.1840130317-BIB23","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/jbmr.5650030414","volume":"3","author":"Plourde","year":"1988","journal-title":"A structure-function study in the rat. J Bone Miner Res"},{"key":"10.1002\/hep.1840130317-BIB24","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1152\/ajplegacy.1963.204.5.833","volume":"204","author":"Guroff","year":"1963","journal-title":"Am J Physiol"},{"key":"10.1002\/hep.1840130317-BIB25","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1016\/S0016-5085(82)80126-1","volume":"83","author":"Proctor","year":"1982","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840130317-BIB26","first-page":"320","volume":"64","author":"Proctor","year":"1983","journal-title":"Br J Exp Pathol"},{"key":"10.1002\/hep.1840130317-BIB27","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1113\/jphysiol.1975.sp010973","volume":"248","author":"LaBrecque","year":"1975","journal-title":"J Physiol (Lond)"},{"key":"10.1002\/hep.1840130317-BIB28","first-page":"186","volume":"12","author":"Higgins","year":"1931","journal-title":"Arch Pathol"},{"key":"10.1002\/hep.1840130317-BIB29","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1210\/endo-126-6-2947","volume":"126","author":"\u00c9thier","year":"1990","journal-title":"Endocrinology"},{"key":"10.1002\/hep.1840130317-BIB30","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1042\/bj0620315","volume":"62","author":"Burton","year":"1956","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840130317-BIB31","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1083\/jcb.43.3.506","volume":"43","author":"Berry","year":"1969","journal-title":"J Cell Biol"},{"key":"10.1002\/hep.1840130317-BIB32","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1042\/bj2550091","volume":"255","author":"Benbrahim","year":"1988","journal-title":"Biochem J"},{"key":"10.1002\/hep.1840130317-BIB33","doi-asserted-by":"crossref","first-page":"2370","DOI":"10.1016\/S0021-9258(20)82244-3","volume":"239","author":"Omura","year":"1964","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840130317-BIB34","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1177\/37.3.2465335","volume":"37","author":"Gascon-Barr\u00e9","year":"1989","journal-title":"J Histochem Cytochem"},{"key":"10.1002\/hep.1840130317-BIB35","first-page":"99","volume-title":"Applied regression analysis and other multivariate methods","author":"Kleinbaun","year":"1978","unstructured":". Applied regression analysis and other multivariate methods. North Scituate, MA: Duxbury Press, 1978; 99\u2013106."},{"key":"10.1002\/hep.1840130317-BIB36","first-page":"309","volume-title":"Statistical principles in experimental design","author":"Winer","year":"1971","unstructured":"Statistical principles in experimental design. New York: McGraw-Hill, 1971; 309."},{"key":"10.1002\/hep.1840130317-BIB37","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1210\/jcem-69-6-1234","volume":"69","author":"Diamond","year":"1989","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1002\/hep.1840130317-BIB38","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1172\/JCI112636","volume":"78","author":"Bikle","year":"1986","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840130317-BIB39","first-page":"29","volume":"3","author":"Meyer","year":"1978","journal-title":"J Mol Med"},{"key":"10.1002\/hep.1840130317-BIB40","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1136\/gut.19.4.290","volume":"19","author":"Jung","year":"1978","journal-title":"Gut"},{"key":"10.1002\/hep.1840130317-BIB41","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1042\/cs0630461","volume":"63","author":"Davie","year":"1982","journal-title":"Clin Sci"},{"key":"10.1002\/hep.1840130317-BIB42","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/S0140-6736(77)92553-3","volume":"1","author":"Stamp","year":"1977","journal-title":"Lancet"},{"key":"10.1002\/hep.1840130317-BIB43","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0026-0495(85)90007-1","volume":"34","author":"Gascon-Barr\u00e9","year":"1985","journal-title":"Metabolism"},{"key":"10.1002\/hep.1840130317-BIB44.1","volume":"251","author":"Gascon-Barr\u00e9","year":"1986","journal-title":"Am J Physiol"},{"volume":"14","journal-title":"Gastroenterol Liver Physiol","first-page":"g627","key":"10.1002\/hep.1840130317-BIB44.2"},{"key":"10.1002\/hep.1840130317-BIB45","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1016\/0016-5085(79)90401-3","volume":"76","author":"Wood","year":"1979","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840130317-BIB46","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1038\/icb.1984.29","volume":"62","author":"Farrell","year":"1984","journal-title":"Aust J Exp Med Sci"},{"key":"10.1002\/hep.1840130317-BIB47","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/0016-5085(85)90759-0","volume":"89","author":"Farrell","year":"1985","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840130317-BIB48","first-page":"117","volume":"3","author":"Murray","year":"1987","journal-title":"Mol Pharmacol"},{"key":"10.1002\/hep.1840130317-BIB49","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1016\/0006-2952(86)90298-4","volume":"35","author":"Murray","year":"1986","journal-title":"Biochem Pharmacol"},{"key":"10.1002\/hep.1840130317-BIB50","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0006-2952(85)90037-1","volume":"34","author":"Babany","year":"1985","journal-title":"Biochem Pharamcol"},{"key":"10.1002\/hep.1840130317-BIB51","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1016\/0006-2952(78)90330-1","volume":"27","author":"Villeneuve","year":"1978","journal-title":"Biochem Pharmacol"},{"key":"10.1002\/hep.1840130317-BIB52","doi-asserted-by":"crossref","first-page":"7738","DOI":"10.1016\/S0021-9258(18)43337-6","volume":"256","author":"Howard","year":"1981","journal-title":"J Biol Chem"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840130317","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199103000-00016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:06:31Z","timestamp":1733051191000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199103000-00016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3]]},"references-count":53,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840130317","relation":{},"ISSN":["0270-9139"],"issn-type":[{"type":"print","value":"0270-9139"}],"subject":[],"published":{"date-parts":[[1991,3]]}}}