{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:38:43Z","timestamp":1760981923271,"version":"3.30.0"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":8492,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>We have previously isolated from 13 cholesterol gallstones a low molecular weight acidic bili\u2013protein that inhibited the precipitation of calcium carbonate <jats:italic toggle=\"yes\">in vitro.<\/jats:italic> We now report the isolation of a similar protein from seven black pigment gallstones. Cholesterol was removed from the stones by Soxhlet apparatus with methyl <jats:italic toggle=\"yes\">t<\/jats:italic>\u2013butyl ether, and bile acids were extracted with methanol. The protein was purified by sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis after demineralization of the stones with ethylenediaminetetraacetate. Structural and functional properties of the protein from the black stones that were similar to the protein from the cholesterol stones included the following: (a) an apparent molecular weight of about 5 kD; (b) a high content of acidic (19.8%) and hydrophobic (50.1%) amino acids with a low content of basic residues (8.4%) and little sulfide\u2013containing amino acids (1.9%); (c) an inhibitory effect on both the initiation and growth of calcium carbonate crystals <jats:italic toggle=\"yes\">in vitro<\/jats:italic>; and (d) very tight (possibly covalent) binding of a diazo\u2013positive yellow pigment, presumably bilirubin, with maximum spectral absorbance at 410 nm. The structural and functional similarities of these bili\u2013proteins from black pigment and cholesterol gallstones and their striking effects on calcium carbonate precipitation <jats:italic toggle=\"yes\">in vitro<\/jats:italic> suggest that they play a common role in the regulation of precipitation of calcium salts during the formation of both types of gallstones. (Hepatology 1992;15:1079-1085).<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840150618","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T00:42:06Z","timestamp":1172968926000},"page":"1079-1085","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":24,"title":["Isolation of A Calcium\u2013Regulatory Protein From Black Pigment Gallstones: Similarity With A Protein From Cholesterol Gallstones"],"prefix":"10.1097","volume":"15","author":[{"given":"Masayuki","family":"Okido","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuji","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald J.","family":"Ostrow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840150618-BIB1","doi-asserted-by":"crossref","first-page":"215s","DOI":"10.1002\/hep.1840040840","volume":"4","author":"Ostrow","year":"1984","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150618-BIB2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1055\/s-2008-1040553","volume":"8","author":"Cahalane","year":"1988","journal-title":"Semin Liver Dis"},{"key":"10.1002\/hep.1840150618-BIB3","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1055\/s-2008-1040471","volume":"10","author":"Crowther","year":"1990","journal-title":"Semin Liver Dis"},{"key":"10.1002\/hep.1840150618-BIB4","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/0016-5085(84)90073-8","volume":"87","author":"Ohkubo","year":"1984","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840150618-BIB5","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/0006-291X(80)91155-9","volume":"93","author":"Zilm","year":"1980","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1002\/hep.1840150618-BIB6","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0016-5085(78)90358-X","volume":"74","author":"Boonyapisit","year":"1978","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840150618-BIB7","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/0002-9610(89)90423-6","volume":"157","author":"Dawes","year":"1989","journal-title":"Am J Surg"},{"key":"10.1002\/hep.1840150618-BIB8","first-page":"219s","volume":"12","author":"Ostrow","year":"1990","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150618-BIB9","first-page":"206s","volume":"12","author":"Moore","year":"1990","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150618-BIB10","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1172\/JCI114389","volume":"84","author":"Shimizu","year":"1989","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150618-BIB11","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/978-94-009-7944-4_25","volume-title":"Biomineralization and biological metal accumulation: biological and geological","author":"Veis","year":"1983","unstructured":". Bone and tooth formation: insights into mineralization strategies. In: , eds. Biomineralization and biological metal accumulation: biological and geological. Dodrecht, The Netherlands: D. Riedel Co., 1983: 273\u2013284."},{"key":"10.1002\/hep.1840150618-BIB12","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1002\/hep.1840020624","volume":"2","author":"Trotman","year":"1982","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150618-BIB13","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/BF01536030","volume":"33","author":"Yamashita","year":"1988","journal-title":"Dig Dis Sci"},{"key":"10.1002\/hep.1840150618-BIB14","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1002\/hep.1840150618-BIB15","first-page":"31","volume-title":"Bile pigments and jaundice: molecular, metabolic and medical aspects","author":"Blanckaert","year":"1986","unstructured":". Analysis and preparation of bilirubins and biliverdins. In: ed. Bile pigments and jaundice: molecular, metabolic and medical aspects. 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