{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:38:21Z","timestamp":1773290301267,"version":"3.50.1"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":8492,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Mitochondrial metabolism was studied in liver mitochondria isolated from rats with secondary biliary cirrhosis induced by bile duct ligation for 5 wk. State 3 oxidation rates were decreased in mitochondrial preparations from bile duct\u2014ligated rats as compared with sham\u2013operated control rats by 63% and 42% using \u03b2\u2013hydroxybutyrate and succinate as substrates, respectively. In contrast, when the substrate was ascorbate\/<jats:italic toggle=\"yes\">N<\/jats:italic>, <jats:italic toggle=\"yes\">N<\/jats:italic>, <jats:italic toggle=\"yes\">N<\/jats:italic>\u2032, <jats:italic toggle=\"yes\">N<\/jats:italic>\u2032\u2013tetramethyl\u2013<jats:italic toggle=\"yes\">p<\/jats:italic>\u2013phenylenediamine state 3 oxidation rates were not affected by bile duct ligation. Oxidation rates after uncoupling with dinitrophenol were decreased for both \u03b2\u2013hydroxybutyrate and succinate as substrates in mitochondria from bile duct\u2014ligated rats. The phosphate potential was reduced in mitochondria from bile duct\u2014ligated rats (12.5 \u00b1 0.5 vs. 13.6 \u00b1 0.2 kcal in control and bile duct\u2014ligated rats, respectively; p&lt;0.05). The inner mitochondrial membrane of liver mitochondria from rats with secondary biliary cirrhosis contained three times more cholesterol as compared with control rats, whereas the phospholipid composition was essentially unchanged. Mitochondrial protein content expressed per liver (calculated on the basis of activities of mitochondrial enzymes determined in liver homogenate and in isolated mitochondria) was increased by 50% in bile duct\u2014ligated rats as compared with control rats. In conclusion, the function of the electron transport chain in liver mitochondria isolated from rats with secondary biliary cirrhosis is impaired. This decrease could be related to altered lipid composition of the inner mitochondrial membrane. (Hepatology 1992;15:1160\u20131166).<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840150630","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T00:42:06Z","timestamp":1172968926000},"page":"1160-1166","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":67,"title":["Reduced Activity of the Electron Transport Chain in Liver Mitochondria Isolated From Rats With Secondary Biliary Cirrhosis"],"prefix":"10.1097","volume":"15","author":[{"given":"Stefan","family":"Kr\u00e4henb\u00fchl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Stucki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg","family":"Reichen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840150630-BIB1","first-page":"599","volume":"52","author":"Diaz Gil","year":"1977","journal-title":"Clin Sci Mol Med"},{"key":"10.1002\/hep.1840150630-BIB2","first-page":"101","volume":"19","author":"Yuki","year":"1984","journal-title":"Alcohol Alcohol"},{"key":"10.1002\/hep.1840150630-BIB3","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0022-4804(84)90201-4","volume":"37","author":"Jikko","year":"1984","journal-title":"J Surg Res"},{"key":"10.1002\/hep.1840150630-BIB4","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1016\/0006-2952(89)90305-5","volume":"38","author":"Kr\u00e4henb\u00fchl","year":"1989","journal-title":"Biochem Pharmacol"},{"key":"10.1002\/hep.1840150630-BIB5","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1600-0773.1984.tb01974.x","volume":"55","author":"Moeller","year":"1984","journal-title":"Acta Pharmacol Toxicol"},{"key":"10.1002\/hep.1840150630-BIB6","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1002\/hep.1840150631","volume":"15","author":"Kr\u00e4henb\u00fchl","year":"1992","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150630-BIB7","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1002\/hep.1840100513","volume":"10","author":"Michaletz","year":"1989","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150630-BIB8","first-page":"94","volume-title":"Methods in enzymology","author":"Johnson","year":"1967","unstructured":". Isolation of liver or kidney mitochondria. In: , eds. Methods in enzymology. New York: Academic Press, 1967: 94\u2013101."},{"key":"10.1002\/hep.1840150630-BIB9","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1021\/bi00869a041","volume":"5","author":"Graven","year":"1966","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840150630-BIB10","first-page":"181","volume-title":"Methods in enzymology","author":"Gregg","year":"1967","unstructured":"Preparation and assay of phosphorylating submitochondrial particles from rat liver prepared by drastic sonication. In: , eds. Methods in enzymology. New York: Academic Press, 1967: 181\u2013185."},{"key":"10.1002\/hep.1840150630-BIB11","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0301-4622(84)85014-0","volume":"19","author":"Stucki","year":"1984","journal-title":"Biophys Chem"},{"key":"10.1002\/hep.1840150630-BIB12","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/0006-3002(62)91132-0","volume":"57","author":"Packer","year":"1962","journal-title":"Biochim Biophys Acta"},{"key":"10.1002\/hep.1840150630-BIB13","first-page":"41","volume-title":"Methods in enzymology","author":"Estabrook","year":"1967","unstructured":"Mitochondrial respiratory control and the polarographic measurement of ADP: O ratios. In: , eds. Methods in enzymology. New York: Academic Press, 1967: 41\u201347."},{"key":"10.1002\/hep.1840150630-BIB14","first-page":"346","volume-title":"Methods of enzymatic analysis","author":"Trautschold","year":"1983","unstructured":", . ATP determination with hexokinase and glucose-6-phosphate dehydrogenase. In: , , eds. Methods of enzymatic analysis. Weinheim, Germany: Verlag Chemie, 1983; 346\u2013356."},{"key":"10.1002\/hep.1840150630-BIB15","first-page":"365","volume-title":"Methods of enzymatic analysis","author":"Jawork","year":"1983","unstructured":". Determination of ADP and AMP: UV-method. In: , , eds. Methods of enzymatic analysis. Weinheim, Germany: Verlag Chemie, 1983: 365\u2013369."},{"key":"10.1002\/hep.1840150630-BIB16","doi-asserted-by":"crossref","first-page":"4030","DOI":"10.1021\/bi00851a033","volume":"7","author":"Atkins","year":"1968","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840150630-BIB17","first-page":"245","volume-title":"Methods of enzymology","author":"Wharton","year":"1967","unstructured":". Cytochrome oxidase from beef heart mitochondria. In: , eds. Methods of enzymology. New York: Academic Press, 1967: 245\u2013250."},{"key":"10.1002\/hep.1840150630-BIB18","first-page":"227","volume-title":"Methods of enzymatic analysis","author":"Kusche","year":"1983","unstructured":"Monoamine oxidase. In: , , eds. Methods of enzymatic analysis. Weinheim, Germany: Verlag Chemie, 1983: 227\u2013236."},{"key":"10.1002\/hep.1840150630-BIB19","first-page":"690","volume-title":"Methoden der enzymatischen analyse","author":"Schmidt","year":"1971","unstructured":"Gluatamat-dehydrogenase. In: ed. Methoden der enzymatischen analyse. Weinheim, Germany: Verlag Chemie, 1971: 690\u2013695."},{"key":"10.1002\/hep.1840150630-BIB20","first-page":"790","volume":"93","author":"Scharschmidt","year":"1979","journal-title":"J Lab Clin Med"},{"key":"10.1002\/hep.1840150630-BIB21","first-page":"118","volume-title":"Methods of enzymatic analysis","author":"Vassault","year":"1983","unstructured":"Lactate dehydrogenase: UV method with pyruvate and NADH. In: , , eds. Methods of enzymatic analysis. Weinheim, Germany: Verlag Chemie, 1983; 118\u2013125."},{"key":"10.1002\/hep.1840150630-BIB22","doi-asserted-by":"crossref","first-page":"2652","DOI":"10.1016\/S0021-9258(19)73803-4","volume":"237","author":"Phillips","year":"1962","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840150630-BIB23","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/0009-8981(59)90119-6","volume":"4","author":"Baum","year":"1959","journal-title":"Clin Chim Acta"},{"key":"10.1002\/hep.1840150630-BIB24","first-page":"273","volume-title":"Methods of enzymatic analysis","author":"Aebi","year":"1983","unstructured":"Catalase. In: , , eds. Methods of enzymatic analysis. Weinheim, Germany: Verlag Chemie, 1983: 273\u2013286."},{"key":"10.1002\/hep.1840150630-BIB25","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840150630-BIB26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0168-8278(89)90068-8","volume":"9","author":"Kr\u00e4henb\u00fchl","year":"1989","journal-title":"J Hepatol"},{"key":"10.1002\/hep.1840150630-BIB27","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/S0022-2275(20)38615-6","volume":"28","author":"Reichen","year":"1987","journal-title":"J Lipid Res"},{"key":"10.1002\/hep.1840150630-BIB28","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0021-9673(00)88346-6","volume":"151","author":"Grob","year":"1978","journal-title":"J Chromatogr"},{"key":"10.1002\/hep.1840150630-BIB29","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1042\/cs0650645","volume":"65","author":"Cairns","year":"1983","journal-title":"Clin Sci"},{"key":"10.1002\/hep.1840150630-BIB30","volume-title":"Statistical methods in medical research","author":"Armitage","year":"1987","unstructured":". Statistical methods in medical research. Oxford: Blackwell Scientific Publication, 1987."},{"key":"10.1002\/hep.1840150630-BIB31","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1002\/hep.1840120313","volume":"12","author":"Kr\u00e4henb\u00fchl","year":"1990","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150630-BIB32","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1042\/bj1720539","volume":"172","author":"Ragan","year":"1978","journal-title":"Biochem J"},{"key":"10.1002\/hep.1840150630-BIB33","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1093\/jn\/115.2.190","volume":"115","author":"Wander","year":"1985","journal-title":"J Nutr"},{"key":"10.1002\/hep.1840150630-BIB34","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/S0006-291X(87)80060-8","volume":"144","author":"Bengochea","year":"1987","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1002\/hep.1840150630-BIB35","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1172\/JCI114942","volume":"86","author":"Kr\u00e4henb\u00fchl","year":"1990","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150630-BIB36","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1172\/JCI113066","volume":"80","author":"Hoppel","year":"1987","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150630-BIB37","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1172\/JCI114135","volume":"84","author":"Haller","year":"1989","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150630-BIB38","first-page":"1427","volume":"35","author":"Nishimura","year":"1985","journal-title":"Arzneimittelforschung"},{"key":"10.1002\/hep.1840150630-BIB39","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1002\/hep.1840070308","volume":"7","author":"Gross","year":"1987","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150630-BIB40","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1002\/hep.1840110510","volume":"11","author":"Wensing","year":"1990","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150630-BIB41","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1021\/bi00380a038","volume":"26","author":"Van Blitterswijk","year":"1987","journal-title":"Biochemistry"},{"key":"10.1002\/hep.1840150630-BIB42","doi-asserted-by":"crossref","first-page":"4130","DOI":"10.1073\/pnas.75.9.4130","volume":"75","author":"Davis","year":"1978","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1002\/hep.1840150630-BIB43","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1172\/JCI109620","volume":"64","author":"Keeffe","year":"1979","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150630-BIB44","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1038\/1781335a0","volume":"178","author":"Zetterstrom","year":"1956","journal-title":"Nature"},{"key":"10.1002\/hep.1840150630-BIB45","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/0014-5793(72)80638-0","volume":"27","author":"Noir","year":"1972","journal-title":"FEBS Lett"},{"key":"10.1002\/hep.1840150630-BIB46","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1136\/gut.7.4.409","volume":"7","author":"Schenker","year":"1966","journal-title":"Gut"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840150630","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199206000-00029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:08:21Z","timestamp":1733051301000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199206000-00029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840150630","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1992,6]]}}}