{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:03:42Z","timestamp":1769519022859,"version":"3.49.0"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":8492,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Mitochondrial and cytosolic functions were studied <jats:italic toggle=\"yes\">in vivo<\/jats:italic> and in perfused livers from rats with secondary biliary cirrhosis induced by bile duct ligation for 5 wk and in sham\u2013operated controls. The livers were stereo\u2013logically analyzed, and mitochondrial and cytosolic functions were related to liver structure. Oxygen consumption by perfused livers expressed per stereo\u2013logically determined mitochondrial volume was decreased by 49% in bile duct\u2014ligated rats compared with control rats. Glucose production (expressed per mitochondrial volume) was reduced by more than 90% in bile duct ligation, whereas urea production was not affected. Lactate production, a cytosolic function, was increased fivefold in bile duct ligation, and both the lactate\/pyruvate and the \u03b2\u2013hydroxybutyrate\/acetoacetate ratios were increased in the liver perfusate of bile duct\u2014ligated rats. In comparison with control rats, the stereologically determined mitochondrial volume fraction per hepatocyte was increased by 28% in bile duct\u2014ligated rats. Activities of mitochondrial enzymes expressed per area of mitochondrial membrane or per mitochondrial volume were either unchanged (ATPase, cytochrome c oxidase and glutamate dehydrogenase) or decreased (monoamine oxidase) in bile duct ligation. Thus in comparison with control rats, mitochondrial metabolism is impaired in perfused livers from bile duct\u2014ligated rats; increased mitochondrial volume per hepatocyte may represent a strategy to maintain hepatic energy metabolism in rats with secondary biliary cirrhosis. (Hepatology 1992;15:1167\u20131172).<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840150631","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T00:42:06Z","timestamp":1172968926000},"page":"1167-1172","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":40,"title":["Stereological and Functional Analysis of Liver Mitochondria From Rats With Secondary Biliary Cirrhosis: Impaired Mitochondrial Metabolism and Increased Mitochondrial Content Per Hepatocyte"],"prefix":"10.1097","volume":"15","author":[{"given":"Stefan","family":"Kr\u00e4henb\u00fchl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Kr\u00e4henb\u00fchl-Glauser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Stucki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Gehr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg","family":"Reichen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840150631-BIB1","first-page":"759","volume":"3","author":"Cameron","year":"1932","journal-title":"J Pathol Bacteriol"},{"key":"10.1002\/hep.1840150631-BIB2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1055\/s-2008-1040584","volume":"7","author":"Ludwig","year":"1987","journal-title":"Semin Liv Dis"},{"key":"10.1002\/hep.1840150631-BIB3","first-page":"599","volume":"52","author":"Diaz Gil","year":"1977","journal-title":"Clin Sci Mol Med"},{"key":"10.1002\/hep.1840150631-BIB4","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0022-4804(84)90201-4","volume":"37","author":"Jikko","year":"1984","journal-title":"J Surg Res"},{"key":"10.1002\/hep.1840150631-BIB5","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1016\/0006-2952(89)90305-5","volume":"38","author":"Kr\u00e4henb\u00fchl","year":"1989","journal-title":"Biochem Pharmacol"},{"key":"10.1002\/hep.1840150631-BIB6","first-page":"101","volume":"19","author":"Yuki","year":"1984","journal-title":"Alcohol Alcohol"},{"key":"10.1002\/hep.1840150631-BIB7","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1600-0773.1984.tb01974.x","volume":"55","author":"Moeller","year":"1984","journal-title":"Acta Pharmacol Toxicol"},{"key":"10.1002\/hep.1840150631-BIB8","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/0014-4800(62)90019-9","volume":"1","author":"Steiner","year":"1962","journal-title":"Exp Mol Pathol"},{"key":"10.1002\/hep.1840150631-BIB9","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/BF00320181","volume":"58","author":"Steiner","year":"1962","journal-title":"Z Zellforsch"},{"key":"10.1002\/hep.1840150631-BIB10","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/S0016-5085(62)80052-3","volume":"42","author":"Carruthers","year":"1962","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840150631-BIB11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1620\/tjem.119.9","volume":"119","author":"Yamauchi","year":"1976","journal-title":"Tohoku J Exp Med"},{"key":"10.1002\/hep.1840150631-BIB12","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1002\/hep.1840070308","volume":"7","author":"Gross","year":"1987","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150631-BIB13","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1172\/JCI112596","volume":"78","author":"Reichen","year":"1986","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150631-BIB14","first-page":"476","volume-title":"Methods of enzymology","author":"Williamson","year":"1969","unstructured":". d-3\u03b2-hydroxybutyrate: determination with 3\u03b2-hydroxybutyrate dehydrogenase. In: , ed. Methods of enzymology. New York: Academic Press, 1969: 476\u2013478."},{"key":"10.1002\/hep.1840150631-BIB15","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0009-8981(71)90486-4","volume":"33","author":"Olsen","year":"1971","journal-title":"Clin Chim Acta"},{"key":"10.1002\/hep.1840150631-BIB16","first-page":"690","volume-title":"Methoden der enzymatischen analyse","author":"Schmidt","year":"1971","unstructured":"Gluatamat-dehydrogenase. In: ed. Methoden der enzymatischen analyse. Weinheim, Germany: Verlag Chemie, 1971: 690\u2013695."},{"key":"10.1002\/hep.1840150631-BIB17","first-page":"790","volume":"93","author":"Scharschmidt","year":"1979","journal-title":"J Lab Clin Med"},{"key":"10.1002\/hep.1840150631-BIB18","first-page":"245","volume-title":"Methods of enzymology","author":"Wharton","year":"1967","unstructured":". Cytochrome oxidase from beef heart mitochondria. In: , ed. Methods of enzymology. New York: Academic Press, 1967: 245\u2013250."},{"key":"10.1002\/hep.1840150631-BIB19","first-page":"227","volume-title":"Methods of enzymatic analysis","author":"Kusche","year":"1983","unstructured":"Monoamine oxidase. In: , , ed. Methods of enzymatic analysis. Weinheim, Germany: Verlag Chemie, 1983: 227\u2013236."},{"key":"10.1002\/hep.1840150631-BIB20","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1365-2818.1981.tb01265.x","volume":"122","author":"Cruz-Orive","year":"1981","journal-title":"J Microsc"},{"key":"10.1002\/hep.1840150631-BIB21","volume-title":"Stereological methods","author":"Weibel","year":"1979","unstructured":"Stereological methods. London: Academic Press, 1979."},{"key":"10.1002\/hep.1840150631-BIB22","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1073\/pnas.86.5.1583","volume":"86","author":"Schwerzmann","year":"1989","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1002\/hep.1840150631-BIB23","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1002\/hep.1840120313","volume":"12","author":"Kr\u00e4henb\u00fchl","year":"1990","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150631-BIB24","volume-title":"Statistical methods in medical research","author":"Armitage","year":"1987","unstructured":". Statistical methods in medical research. Oxford, UK: Blackwell Scientific Publication, 1987."},{"key":"10.1002\/hep.1840150631-BIB25","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1042\/bj1030514","volume":"103","author":"Williamson","year":"1966","journal-title":"Biochem J"},{"key":"10.1002\/hep.1840150631-BIB26","first-page":"16","volume":"10","author":"Gall","year":"1990","journal-title":"Liver"},{"key":"10.1002\/hep.1840150631-BIB27","first-page":"563","volume":"19","author":"Wijburg","year":"1989","journal-title":"Biochem Int"},{"key":"10.1002\/hep.1840150631-BIB28","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/BF01799394","volume":"13","author":"Wijburg","year":"1990","journal-title":"J Inherited Metab Dis"},{"key":"10.1002\/hep.1840150631-BIB29","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1172\/JCI113066","volume":"80","author":"Hoppel","year":"1987","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150631-BIB30","doi-asserted-by":"crossref","first-page":"2622","DOI":"10.1016\/S0021-9258(18)99617-1","volume":"242","author":"Exton","year":"1967","journal-title":"J Biol Chem"},{"key":"10.1002\/hep.1840150631-BIB31","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1146\/annurev.bi.52.070183.003153","volume":"52","author":"Hers","year":"1983","journal-title":"Annu Rev Biochem"},{"key":"10.1002\/hep.1840150631-BIB32","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1002\/bjs.1800590716","volume":"59","author":"Lee","year":"1972","journal-title":"Br J Surg"},{"key":"10.1002\/hep.1840150631-BIB33","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/0006-2952(79)90240-5","volume":"28","author":"Perez","year":"1979","journal-title":"Biochem Pharmacol"},{"key":"10.1002\/hep.1840150631-BIB34","first-page":"495","volume-title":"Biochemistry","author":"Stryer","year":"1988","unstructured":"Biochemistry. New York: W.H. Freeman and Company, 1988: 495\u2013517."},{"key":"10.1002\/hep.1840150631-BIB35","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1002\/hep.1840140528","volume":"14","author":"Kr\u00e4henb\u00fchl","year":"1991","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840150631-BIB36","first-page":"512","volume":"35","author":"Belinsky","year":"1989","journal-title":"Mol Pharmacol"},{"key":"10.1002\/hep.1840150631-BIB37","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1172\/JCI114135","volume":"84","author":"Haller","year":"1989","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840150631-BIB38","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1172\/JCI114942","volume":"86","author":"Kr\u00e4henb\u00fchl","year":"1990","journal-title":"J Clin Invest"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840150631","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199206000-00030","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:07:57Z","timestamp":1733051277000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199206000-00030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840150631","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1992,6]]}}}