{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T10:21:05Z","timestamp":1779358865962,"version":"3.51.4"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":8370,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:title>\n                           <jats:bold>ABSTRACT<\/jats:bold>\n                        <\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>An autoantibody to liver cytosol was previously described in childhood autoimmune chronic active hepatitis type 2. The antigen, liver cytosol antigen type 1, was for the first time partially purified using gel filtration and ion exchange chromatography, and it was characterized using immunodiffusion, immunoblot and sodium dodecyl sulfate\u2014polyacrylamide gel electrophoresis analysis of the immunoprecipitate. Immunoblot detected a unique antigenic peptide at 62 kD from human cytosol and at 58 kD from rat cytosol. The same peptides were also detected when immuno\u2013precipitates of liver cytosol antigen type 1 and autoantibodies to liver cytosol antigen were submitted to sodium dodecyl sulfate\u2014polyacrylamide gel electrophoresis. A polymeric structure, probably a tetramer, is suggested for native liver cytosol antigen type 1 because in gel filtration chromatography liver cytosol antigen type 1 was eluted as a protein of a molecular weight between 240 and 290 kD when human liver cytosol was fractionated and between 220 and 270 kD from rat liver cytosol. Liver cytosol antigen type 1 is probably poor in carbohydrates because it was not stained by periodic acid\u2014Schiff stain. The autoantibodies to liver cytosol were frequently found in association with antiliver kidney microsomal autoantibodies type 1, which are directed against the cytochrome P\u2013450 of the IID6 subfamily. Antiliver kidney microsomal autoantibodies type 1 but not antiliver cytosol autoantibodies were found in association with antibodies to hepatitis C virus. Autoantibodies to liver cytosol antigen type 1 seem to be a more specific marker for autoimmune hepatitis type 2 than antiliver kidney microsomal antibodies type 1 autoantibodies. (HEPATOLOGY 1992;16:892-898.)<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.1840160407","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T01:22:37Z","timestamp":1172971357000},"page":"892-898","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":99,"title":["Characterization of the Liver Cytosol Antigen Type 1 Reacting With Autoantibodies in Chronic Active Hepatitis"],"prefix":"10.1097","volume":"16","author":[{"given":"Nisen","family":"Abuaf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Johanet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale","family":"Chretien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Martini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuelle","family":"Soulier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syria","family":"Laperche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean Claude","family":"Homberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1002\/hep.1840160407-BIB1","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S0140-6736(87)92024-1","volume":"1","author":"Manns","year":"1987","journal-title":"Lancet"},{"key":"10.1002\/hep.1840160407-BIB2","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/0168-8278(90)90119-C","volume":"11","author":"W\u00e4chter","year":"1990","journal-title":"J Hepatol"},{"key":"10.1002\/hep.1840160407-BIB3","first-page":"253","volume":"20","author":"Johanet","year":"1987","journal-title":"Rev Inst Pasteur"},{"key":"10.1002\/hep.1840160407-BIB4","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1002\/hep.1840080632","volume":"8","author":"Martini","year":"1988","journal-title":"HEPATOLOGY"},{"key":"10.1002\/hep.1840160407-BIB5","first-page":"331","volume":"15","author":"Rizzetto","year":"1973","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840160407-BIB6","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1002\/hep.1840070626","volume":"7","author":"Homberg","year":"1987","journal-title":"HEPATOLOGY"},{"key":"10.1002\/hep.1840160407-BIB7","first-page":"561","volume":"55","author":"Homberg","year":"1984","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840160407-BIB8","first-page":"232","volume":"54","author":"Crivelli","year":"1983","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840160407-BIB9","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1084\/jem.161.5.1231","volume":"161","author":"Alvarez","year":"1985","journal-title":"J Exp Med"},{"key":"10.1002\/hep.1840160407-BIB10","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1084\/jem.168.2.801","volume":"168","author":"Gueguen","year":"1988","journal-title":"J Exp Med"},{"key":"10.1002\/hep.1840160407-BIB11","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1016\/0016-5085(88)90368-X","volume":"95","author":"Waxman","year":"1988","journal-title":"Gastroenterology"},{"key":"10.1002\/hep.1840160407-BIB12","doi-asserted-by":"crossref","first-page":"8256","DOI":"10.1073\/pnas.85.21.8256","volume":"85","author":"Zanger","year":"1988","journal-title":"Proc Natl Acad Sci, USA"},{"key":"10.1002\/hep.1840160407-BIB13","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0006-291X(89)92435-2","volume":"159","author":"Kiffel","year":"1989","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1002\/hep.1840160407-BIB14","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1172\/JCI113949","volume":"83","author":"Manns","year":"1989","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840160407-BIB15","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1073\/pnas.84.2.551","volume":"84","author":"Beaune","year":"1987","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1002\/hep.1840160407-BIB16","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1073\/pnas.86.1.322","volume":"86","author":"Satoh","year":"1989","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1002\/hep.1840160407-BIB17","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1172\/JCI114660","volume":"85","author":"Bourdi","year":"1990","journal-title":"J Clin Invest"},{"key":"10.1002\/hep.1840160407-BIB18","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/0140-6736(90)90070-L","volume":"335","author":"Lenzi","year":"1990","journal-title":"Lancet"},{"key":"10.1002\/hep.1840160407-BIB19","first-page":"93","volume-title":"Autoimmune liver disease","author":"Abuaf","year":"1991","unstructured":", . Autoantibodies in autoimmune chronic active hepatitis. In: , eds. Autoimmune liver disease. New York: Raven Press, 1991: 93\u2013109."},{"key":"10.1002\/hep.1840160407-BIB20","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1042\/bj0600604","volume":"60","author":"De Duve","year":"1955","journal-title":"Biochem J"},{"key":"10.1002\/hep.1840160407-BIB21","first-page":"865","volume":"18","author":"The","year":"1970","journal-title":"Immunology"},{"key":"10.1002\/hep.1840160407-BIB22","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1365-2249.1990.tb05402.x","volume":"82","author":"Dighiero","year":"1990","journal-title":"Clin Exp Immunol"},{"key":"10.1002\/hep.1840160407-BIB23","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"Laemmli","year":"1979","journal-title":"Nature"},{"key":"10.1002\/hep.1840160407-BIB24","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1002\/hep.1840160407-BIB25","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1002\/anr.1780321210","volume":"32","author":"Rader","year":"1989","journal-title":"Arthritis Rheum"},{"key":"10.1002\/hep.1840160407-BIB26","first-page":"127","volume":"32","author":"Taylor-Albert","year":"1989","journal-title":"Arthritis Rheum"},{"key":"10.1002\/hep.1840160407-BIB27","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/0168-8278(91)90874-B","volume":"13","author":"Todros","year":"1991","journal-title":"J Hepatol"},{"key":"10.1002\/hep.1840160407-BIB28","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1016\/0140-6736(90)90870-B","volume":"335","author":"McFarlane","year":"1990","journal-title":"Lancet"},{"key":"10.1002\/hep.1840160407-BIB29","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1016\/0140-6736(90)91163-5","volume":"335","author":"Dussaix","year":"1990","journal-title":"Lancet"},{"key":"10.1002\/hep.1840160407-BIB30","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/0140-6736(91)90418-O","volume":"338","author":"Lenzi","year":"1991","journal-title":"Lancet"},{"key":"10.1002\/hep.1840160407-BIB31","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1002\/hep.1840160304","volume":"16","author":"Lunel","year":"1992","journal-title":"Hepatology"},{"key":"10.1002\/hep.1840160407-BIB32","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1002\/jmv.1890340405","volume":"34","author":"Garson","year":"1991","journal-title":"J Med Virol"},{"key":"10.1002\/hep.1840160407-BIB33","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/art.1780330307","volume":"33","author":"Ben-Chetrit","year":"1990","journal-title":"Arthritis Rheum"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.1840160407","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199210000-00006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:05:31Z","timestamp":1733051131000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199210000-00006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.1840160407","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1992,10]]}}}