{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:30:59Z","timestamp":1791232259851,"version":"4.1.0"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":4048,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Intrahepatic biliary cell differentiation takes place in periportal hepatoblasts under the influence of the subjacent connective tissue, the mechanism of which is still unclear. This study was undertaken to analyze the immunolocalization of extracellular matrix components and their cellular receptors during mouse liver development, with special attention given to biliary differentiation and vascular development. In young fetal mouse liver, primitive structures of sinusoids were developed between hepatic cords associated with hematopoietic cells demonstrated by immunohistochemistry of basal laminar components, the \u03b16 integrin subunit, and PECAM-1. Portal veins and hepatic veins showed different staining intensities of \u03b12, \u03b13, and \u03b16 integrin subunits from early stages of development. Anti-\u03b24 integrin subunit antibodies reacted with portal veins, but not with hepatic veins after perinatal stages. Their different phenotypes may be related to the preferential differentiation of periportal bile ducts. In intrahepatic bile duct development, periportal hepatoblasts adjacent to the connective tissue were immunostained for each basal laminar component on the basal side at almost the same time; \u03b13, \u03b15, \u03b16, and \u03b24 integrin subunits were immunohistochemically detectable later than the basal laminar components. These staining patterns of intrahepatic bile duct cells clearly differed from those of extrahepatic bile duct cells from the beginning of their development, suggesting that these ducts are of different origins. <jats:underline>In conclusion<\/jats:underline>, the vascular structures, including sinusoids, portal veins, and hepatic veins, develop from early stages of liver development, and the extracellular matrix components may play important roles in biliary differentiation and vascular development. <jats:italic toggle=\"yes\">Supplementary material for this article can be found on the Hepatology website<\/jats:italic> (http:\/\/interscience.wiley.com\/jpages\/0270-9139\/suppmat\/index.html). (Hepatology 2004;40:346-355.)<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.20303","type":"journal-article","created":{"date-parts":[[2004,8,13]],"date-time":"2004-08-13T16:10:22Z","timestamp":1092413422000},"page":"346-355","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":79,"title":["Immunolocalization of extracellular matrix components and integrins during mouse liver development"],"prefix":"10.1097","volume":"40","author":[{"given":"Nobuyoshi","family":"Shiojiri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Sugiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-12-20241201","first-page":"26112620","article-title":"Cell lineages and oval cell progenitors in rat liver development.","volume":"51","author":"Shiojiri","year":"1991","journal-title":"Cancer Res"},{"key":"R2-12-20241201","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1038\/labinvest.3780208","article-title":"Cell lineage analysis during liver development using spfash-heterozygous mouse.","volume":"81","author":"Shiojiri","year":"2001","journal-title":"Lab Invest"},{"key":"R3-12-20241201","doi-asserted-by":"crossref","first-page":"256267","DOI":"10.1007\/BF01891531","article-title":"Uber die gestaltende Wechselwirkung zwischen dem Epithel und dem Mesenchym, zugleich ein Beitrag zur Histogenese der sogenannten Gallengangswucherungen.","volume":"292","author":"Doljanski","year":"1934","journal-title":"Virchows Arch Pathol Anat Physiol"},{"key":"R4-12-20241201","doi-asserted-by":"crossref","first-page":"153163","DOI":"10.1007\/BF00572540","article-title":"Secondary joining of the bile ducts during the hepatogenesis of the mouse embryo.","volume":"177","author":"Shiojiri","year":"1987","journal-title":"Anat Embryol"},{"key":"R5-12-20241201","first-page":"5259","article-title":"Intrahepatic bile duct development in the rat: a cytokeratin-immunohistochemical study.","volume":"59","author":"Van Eyken","year":"1988","journal-title":"Lab Invest"},{"key":"R6-12-20241201","doi-asserted-by":"crossref","first-page":"15861595","DOI":"10.1002\/hep.1840080619","article-title":"The development of the intrahepatic bile ducts in man: a keratin-immunohistochemical study.","volume":"8","author":"Van Eyken","year":"1988","journal-title":"HEPATOLOGY"},{"key":"R7-12-20241201","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1007\/BF00213597","article-title":"Preferential differentiation of the bile ducts along the portal vein in the development of mouse liver.","volume":"185","author":"Shiojiri","year":"1992","journal-title":"Anat Embryol"},{"key":"R8-12-20241201","doi-asserted-by":"crossref","first-page":"388397","DOI":"10.1002\/hep.1840200219","article-title":"Profiles of expression of carbohydrate chain structures during human intrahepatic bile duct development and maturation: a lectin-histochemical and immunohistochemical study.","volume":"20","author":"Terada","year":"1994","journal-title":"HEPATOLOGY"},{"key":"R9-12-20241201","doi-asserted-by":"crossref","first-page":"143150","DOI":"10.1111\/j.1365-2559.1994.tb01570.x","article-title":"Expression of tenascin, type IV collagen and laminin during human intrahepatic bile duct development and in intrahepatic cholangiocarcinoma.","volume":"25","author":"Terada","year":"1994","journal-title":"Histopathology"},{"key":"R10-12-20241201","doi-asserted-by":"crossref","first-page":"10751082","DOI":"10.1242\/dev.129.4.1075","article-title":"A mouse model of Alagille syndrome: Notch2 as a genetic modifier of Jag1 haploinsufficiency.","volume":"129","author":"McCright","year":"2002","journal-title":"Development"},{"key":"R12-12-20241201","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1111\/j.1749-6632.1963.tb36945.x","article-title":"Histogenesis of the liver.","volume":"111","author":"Wilson","year":"1963","journal-title":"Ann N Y Acad Sci"},{"key":"R13-12-20241201","doi-asserted-by":"crossref","first-page":"507530","DOI":"10.1002\/ar.1091510403","article-title":"An electron microscope study of developing bile canaliculi in the rat.","volume":"151","author":"Wood","year":"1965","journal-title":"Anat Rec"},{"key":"R14-12-20241201","doi-asserted-by":"crossref","first-page":"85107","DOI":"10.1002\/aja.1001330106","article-title":"A morphological study of the development of the human liver. 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