{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:26:31Z","timestamp":1759332391751,"version":"3.30.0"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":4017,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>It has been reported that autoantibodies to hepatocytes are frequently found in patients with autoimmune hepatitis (AIH). To elucidate the nature of these hepatocyte-specific autoantibodies, we attempted to generate a hepatocyte-specific monoclonal antibody (MoAb) from Epstein-Barr virus-transformed peripheral blood mononuclear cells obtained from a patient with AIH. We established a single clone, 2E3, that continued to produce an immunoglobulin M (IgM) antibody (\u03bb-type). This MoAb had the following properties: it reacted mainly with hepatocyte-derived cell lines, rather than with other cell lines, and it reacted with liver tissue but not with other tissues. By immunoblot analysis, we found that this MoAb recognized a 190 kDa molecule on hepatocytes. The MoAb was able to kill hepatocyte-derived cell lines in the presence of fresh human serum. This cytotoxic effect was completely abrogated by heat inactivation of human serum prior to its addition to cell lines. In addition, an IgM autoantibody that recognized a 190 kDa molecule was also found in patients with AIH but not in those with chronic hepatitis C; its titer correlated significantly with serum alanine aminotransferase (ALT) levels in patients with AIH. <jats:underline>In conclusion<\/jats:underline>, we generated a human MoAb that recognizes a 190 kDa molecule on hepatocytes. Because of its ability to mediate complement-dependent cytotoxicity and the presence of similar IgM autoantibody in patients with AIH, we hypothesize this autoantibody may play a role in the immunopathogenesis of AIH. (Hepatology 2004;40:687-692.)<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.20377","type":"journal-article","created":{"date-parts":[[2004,8,30]],"date-time":"2004-08-30T22:04:11Z","timestamp":1093903451000},"page":"687-692","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["A novel IgM class autoantibody to a hepatocyte-related 190 kDa molecule in patients with type 1 autoimmune hepatitis"],"prefix":"10.1097","volume":"40","author":[{"given":"Katsumi","family":"Yamauchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takaji","family":"Furukawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuko","family":"Takatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshimi","family":"Nakanishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etsuko","family":"Isono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsutoshi","family":"Tokushige","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuji","family":"Komatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Shiratori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"R1-26-20241201","first-page":"381390","article-title":"Purification and characterization of human liver-specific membrane lipoprotein (LSP).","volume":"27","author":"McFarlane","year":"1977","journal-title":"Clin Exp Immunol"},{"key":"R2-26-20241201","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1055\/s-2008-1040641","article-title":"Natural history, clinical features, and treatment of autoimmune hepatitis.","volume":"4","author":"Czaja","year":"1984","journal-title":"Semin Liver Dis"},{"issue":"Suppl 1","key":"R3-26-20241201","first-page":"181197","article-title":"Autoimmune hepatitis.","volume":"32","author":"Obermayer-Straub","year":"2000","journal-title":"J Hepatol"},{"key":"R4-26-20241201","doi-asserted-by":"crossref","first-page":"215222","DOI":"10.1055\/s-2008-1040439","article-title":"The asialoglycoprotein receptor as target structure in autoimmune liver diseases.","volume":"11","author":"Poralla","year":"1991","journal-title":"Semin Liver Dis"},{"key":"R5-26-20241201","doi-asserted-by":"crossref","first-page":"17331740","DOI":"10.1007\/BF02088738","article-title":"Frequency and significance of antibodies to asialoglycoprotein receptor in type 1 autoimmune hepatitis.","volume":"41","author":"Czaja","year":"1996","journal-title":"Dig Dis Sci"},{"key":"R6-26-20241201","doi-asserted-by":"crossref","first-page":"12461249","DOI":"10.1177\/31.10.6350436","article-title":"Antibodies to hepatocyte membrane antigens in chronic liver disease: detection by immunofluorescence after Bouins fixation.","volume":"31","author":"Lee","year":"1983","journal-title":"J Histochem Cytochem"},{"key":"R7-26-20241201","doi-asserted-by":"crossref","first-page":"14981506","DOI":"10.1002\/hep.510230629","article-title":"Cloning of variable regions of an antibody that reacts with the soluble fraction of human liver cells and its possible value in chronic liver disease.","volume":"23","author":"Saito","year":"1996","journal-title":"HEPATOLOGY"},{"key":"R8-26-20241201","doi-asserted-by":"crossref","first-page":"10541066","DOI":"10.1002\/hep.510260438","article-title":"Cytochrome P450 and uridine triphosphate-glucuronosyltransferases: model autoantigens to study drug-induced, virus-induced, and autoimmune liver disease.","volume":"26","author":"Manns","year":"1997","journal-title":"HEPATOLOGY"},{"key":"R9-26-20241201","doi-asserted-by":"crossref","first-page":"11051107","DOI":"10.1002\/hep.510250510","article-title":"Anti-neutrophil cytoplasmic antibodies in type 1 and 2 autoimmune hepatitis.","volume":"25","author":"Zauli","year":"1997","journal-title":"HEPATOLOGY"},{"key":"R10-26-20241201","first-page":"455461","article-title":"Argininosuccinate lyase: a new autoantigen in liver disease.","volume":"114","author":"Pelli","year":"1998","journal-title":"Clin Exp Immunol"},{"key":"R11-26-20241201","first-page":"4147","article-title":"Liver cell-membrane specific antibody in autoimmune hepatitis patients detected by flow cytometry. Hep. Res. (formaly Int. Hep. Commun.","volume":"1","author":"Tokushige","year":"1993","journal-title":")"},{"key":"R12-26-20241201","doi-asserted-by":"crossref","first-page":"846851","DOI":"10.1111\/j.1572-0241.2001.03630.x","article-title":"Clinical significance of autoantibody to hepatocyte membrane antigen in type 1 autoimmune hepatitis.","volume":"96","author":"Sasaki","year":"2001","journal-title":"Am J Gastroenterol"},{"key":"R13-26-20241201","first-page":"5461","article-title":"Liver membrane antibodies (LMA) recognize a 26-kD protein on the hepatocellular surface.","volume":"79","author":"Hopf","year":"1990","journal-title":"Clin Exp Immunol"},{"key":"R14-26-20241201","first-page":"441444","article-title":"Antibody-dependent cell-mediated (K cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis.","volume":"1","author":"Cochrane","year":"1976","journal-title":"Lancet"},{"key":"R15-26-20241201","doi-asserted-by":"crossref","first-page":"5254","DOI":"10.1038\/267052a0","article-title":"Polyclonal Ig production after Epstein-Barr virus infection of human lymphocytes in vitro.","volume":"267","author":"Rosen","year":"1977","journal-title":"Nature"},{"key":"R16-26-20241201","doi-asserted-by":"crossref","first-page":"255262","DOI":"10.1046\/j.1365-2249.2002.01739.x","article-title":"Myelin protein P0-specific IgM producing monoclonal B cell lines were established from polyneuropathy patients with monoclonal gammopathy of undetermined significance (MGUS).","volume":"127","author":"Kvarnstrom","year":"2002","journal-title":"Clin Exp Immunol"},{"key":"R17-26-20241201","doi-asserted-by":"crossref","first-page":"929938","DOI":"10.1016\/S0168-8278(99)80297-9","article-title":"International Autoimmune Hepatitis Group Report: review of criteria for diagnosis of autoimmune hepatitis.","volume":"31","author":"Alvarez","year":"1999","journal-title":"J Hepatol"},{"key":"R18-26-20241201","doi-asserted-by":"crossref","first-page":"14491452","DOI":"10.1038\/4042","article-title":"Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration.","volume":"4","author":"Nagahara","year":"1998","journal-title":"Nat Med"},{"key":"R19-26-20241201","first-page":"24512456","article-title":"A new antibody, anti-210kDa microtubule associated antibody, detected in the serum of patients with various liver diseases and SLE.","volume":"87","author":"Komatsu","year":"1990","journal-title":"Nippon Shokakibyo Gakkai Zasshi"},{"key":"R20-26-20241201","doi-asserted-by":"crossref","first-page":"291304","DOI":"10.1016\/0090-1229(89)90180-3","article-title":"Autoantibodies to isolated human hepatocyte plasma membranes in liver disease. 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