{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:12:18Z","timestamp":1790914338399,"version":"4.1.0"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":3499,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>\n                                 <jats:bold>Low-density lipoprotein (LDL) is an important carrier of plasma cholesterol and triglycerides whose concentration is regulated by the liver parenchymal cells. Abnormal LDL regulation is thought to cause atherosclerosis, while viral binding to LDL has been suggested to facilitate hepatitis C infection. Primary hepatocytes quickly lose the ability to clear LDL during<\/jats:bold>\n                                 <jats:bold>\n                                    in vitro\n                                 <\/jats:bold>\n                                 <jats:bold>culture. Here we show that the coculture of hepatocytes with liver sinusoidal endothelial cells (LSEC) significantly increases the ability of hepatocytes to uptake LDL<\/jats:bold>\n                                 <jats:bold>\n                                    in vitro\n                                 <\/jats:bold>\n                                 <jats:bold>. LDL uptake does not increase when hepatocytes are cocultured with other cell types such as fibroblasts or umbilical vein endothelial cells. We find that LSECs induce the hepatic expression of the LDL receptor and the epidermal growth factor receptor. In addition, while hepatocytes in single culture did not take up hepatitis C virus (HCV)-like particles, the hepatocytes cocultured with LSECs showed a high level of HCV-like particle uptake. We suggest that coculture with LSECs induces the emergence of a sinusoidal surface in primary hepatocytes conducive to the uptake of HCV-like particles.<\/jats:bold>\n                                 <jats:bold>\n                                    In conclusion\n                                 <\/jats:bold>\n                                 <jats:bold>, our findings describe a novel model of polarized hepatocytes<\/jats:bold>\n                                 <jats:bold>\n                                    in vitro\n                                 <\/jats:bold>\n                                 <jats:bold>that can be used for the study of LDL metabolism and hepatitis C infection.<\/jats:bold>\n                                 <jats:bold>\n                                    Supplementary material for this article can be found on the HEPATOLOGY website (\n                                 <\/jats:bold>\n                                 http:\/\/interscience.wiley.com\/jpages\/0270-9139\/suppmat\/index.html\n                                 <jats:bold>\n                                    ).\n                                 <\/jats:bold>\n                                 <jats:bold>(Hepatology 2006;43:257\u2013265.)<\/jats:bold>\n                              <\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.21016","type":"journal-article","created":{"date-parts":[[2006,1,27]],"date-time":"2006-01-27T00:47:29Z","timestamp":1138322849000},"page":"257-265","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":70,"title":["Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes"],"prefix":"10.1097","volume":"43","author":[{"given":"Yaakov","family":"Nahmias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Casali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Barbe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Berthiaume","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin L.","family":"Yarmush","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-8-20241201","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1126\/science.3513311","article-title":"A receptormediated pathway for cholesterol homeostasis.","volume":"232","author":"Brown","year":"1986","journal-title":"Science"},{"key":"R2-8-20241201","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9150(03)00239-9","article-title":"Statins in atherosclerosis: lipidlowering agents with antioxidant capabilities.","volume":"173","author":"Rosenson","year":"2004","journal-title":"Atherosclerosis"},{"key":"R3-8-20241201","doi-asserted-by":"crossref","first-page":"20963","DOI":"10.1074\/jbc.272.34.20963","article-title":"Low density lipoprotein oxidation and its pathobiological significance.","volume":"272","author":"Steinberg","year":"1997","journal-title":"J Biol Chem"},{"key":"R4-8-20241201","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s00726-003-0029-5","article-title":"Scavenger receptors for oxidized and glycated proteins.","volume":"25","author":"Horiuchi","year":"2003","journal-title":"Amino Acids"},{"key":"R5-8-20241201","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/hep.1840140114","article-title":"Boom JHvd, Schouten D, Roholl PJ, Heyde MNvd, Brouwer A, Berkel TJv, et al. Visualization of the interaction of native and modified lipoproteins with parenchymal, endothelial and Kupffer cells from human liver.","volume":"14","author":"KleinherenbrinkStins","year":"1991","journal-title":"HEPATOLOGY"},{"key":"R6-8-20241201","doi-asserted-by":"crossref","first-page":"12766","DOI":"10.1073\/pnas.96.22.12766","article-title":"Hepatitis C virus and other flaviviridae viruses enter cells via low density lipoprotein receptor.","volume":"96","author":"Agnello","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R7-8-20241201","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s004300000032","article-title":"Binding of human lipoproteins (low, very low, high density lipoproteins) to recombinant envelope proteins of hepatitis C virus.","volume":"188","author":"Monazahian","year":"2000","journal-title":"Med Microbiol 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