{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:56:38Z","timestamp":1791176198975,"version":"4.1.0"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":1095,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>\n                                 <jats:bold>The development of human cultured hepatitis C virus (HCV) replication-permissive hepatocarcinoma cell lines has provided important new virological tools to study the mechanisms of HCV infection; however, this experimental model remains distantly related to physiological and pathological conditions. Here, we report the development of a new<\/jats:bold>\n                                 <jats:bold>\n                                    ex vivo\n                                 <\/jats:bold>\n                                 <jats:bold>model using human adult liver slices culture, demonstrating, for the first time, the ability of primary isolates to undergo<\/jats:bold>\n                                 <jats:bold>\n                                    de novo\n                                 <\/jats:bold>\n                                 <jats:bold>viral replication with the production of high-titer infectious virus as well as Japanese fulminant hepatitis type 1, H77\/C3, and Con1\/C3. This experimental model was employed to demonstrate HCV neutralization or HCV inhibition, in a dose-dependent manner, either by cluster of differentiation 81 or envelope protein 2\u2013specific antibodies or convalescent serum from a recovered HCV patient or by antiviral drugs.<\/jats:bold>\n                                 <jats:bold>\n                                    Conclusion\n                                 <\/jats:bold>: <jats:bold>This new<\/jats:bold>\n                                 <jats:bold>\n                                    ex vivo\n                                 <\/jats:bold>\n                                 <jats:bold>model represents a powerful tool for studying the viral life cycle and dynamics of virus spread in native tissue and also allows one to evaluate the efficacy of new antiviral drugs.<\/jats:bold>\n                              <\/jats:p>\n          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