{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:10:20Z","timestamp":1772118620570,"version":"3.50.1"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":35,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003323","name":"Agence Nationale de Recherches sur le Sida et les Hepatites Virales","doi-asserted-by":"publisher","award":["2012\/239, 2012\/318, 2013\/108"],"award-info":[{"award-number":["2012\/239, 2012\/318, 2013\/108"]}],"id":[{"id":"10.13039\/501100003323","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004100","name":"Labex","doi-asserted-by":"publisher","award":["ANR-10-LABX-0028_HEPSYS"],"award-info":[{"award-number":["ANR-10-LABX-0028_HEPSYS"]}],"id":[{"id":"10.13039\/501100004100","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Direction G\u00e9n\u00e9rale de l'Offre de Soins","award":["A12027MS"],"award-info":[{"award-number":["A12027MS"]}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["ERC-2008-AdG-233130-HEPCENT"],"award-info":[{"award-number":["ERC-2008-AdG-233130-HEPCENT"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["FP7 HepaMab"],"award-info":[{"award-number":["FP7 HepaMab"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["Interreg IV FEDER-Hepato-Regio-Net 2012"],"award-info":[{"award-number":["Interreg IV FEDER-Hepato-Regio-Net 2012"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>\n                    Hepatitis C virus (HCV) causes persistent infection in the majority of infected individuals. The mechanisms of persistence and clearance are only partially understood. Antibodies (Abs) against host cell entry receptors have been shown to inhibit HCV infection in cell culture and animal models. In this study, we aimed to investigate whether anti\u2010receptor Abs are induced during infection in humans\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    and whether their presence is associated with outcome of infection. We established an enzyme\u2010linked immunosorbant assay using a recombinant CD81\u2010claudin\u20101 (CLDN1) fusion protein to detect and quantify Abs directed against extracellular epitopes of the HCV CD81\u2010CLDN1 coreceptor complex. The presence of anti\u2010receptor Abs was studied in serum of patients from a well\u2010defined cohort of a single\u2010source HCV outbreak of pregnant women and several control groups, including uninfected pregnant women, patients with chronic hepatitis B and D virus (HBV\/HDV) infection, and healthy individuals. Virus\u2010neutralizing activity of Abs was determined using recombinant cell culture\u2013derived HCV (HCVcc). Our results demonstrate that HCV\u2010infected patients have statistically significantly higher anti\u2010CD81\/CLDN1 Ab titers during the early phase of infection than controls. The titers were significantly higher in resolvers compared to persisters. Functional studies using immunoadsorption and HCV cell culture models demonstrate that HCV\u2010neutralizing anti\u2010receptor Abs are induced in the early phase of HCV infection, but not in control groups.\n                    <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>\n                    : The virus\u2010neutralizing properties of these Abs suggest a role for control of viral infection in conjunction with antiviral responses. Characterization of these anti\u2010receptor Abs opens new avenues to prevent and treat HCV infection. (H\n                    <jats:sc>epatology<\/jats:sc>\n                    2015;62:726\u2013736)\n                  <\/jats:p>","DOI":"10.1002\/hep.27906","type":"journal-article","created":{"date-parts":[[2015,5,23]],"date-time":"2015-05-23T10:30:54Z","timestamp":1432377054000},"page":"726-736","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Acute hepatitis C virus infection induces anti\u2010host cell receptor antibodies with virus\u2010neutralizing properties"],"prefix":"10.1097","volume":"62","author":[{"given":"Rajiv G.","family":"Tawar","sequence":"first","affiliation":[{"name":"Inserm, U1110, Institut des Maladies Virales et H\u00e9patiques,Strasbourg,France"},{"name":"University of Strasbourg,Strasbourg,France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Che C.","family":"Colpitts","sequence":"additional","affiliation":[{"name":"Inserm, U1110, Institut des Maladies Virales et H\u00e9patiques,Strasbourg,France"},{"name":"University of Strasbourg,Strasbourg,France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Timm","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital D\u00fcsseldorf, Heinrich\u2010Heine\u2010University,D\u00fcsseldorf,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Fehm","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics,University Hospital D\u00fcsseldorf, Heinrich\u2010Heine\u2010University,D\u00fcsseldorf,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Roggendorf","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Duisburg\u2010Essen,Essen,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helga","family":"Meisel","sequence":"additional","affiliation":[{"name":"Institute of Medical Virology, Helmut\u2010Ruska\u2010Haus, Charit\u00e9 University Medicine,Berlin,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Meyer","sequence":"additional","affiliation":[{"name":"P\u00f4le de Sant\u00e9 Publique, H\u00f4pitaux Universitaires de Strasbourg,Strasbourg,France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Habersetzer","sequence":"additional","affiliation":[{"name":"Inserm, U1110, Institut des Maladies Virales et H\u00e9patiques,Strasbourg,France"},{"name":"University of Strasbourg,Strasbourg,France"},{"name":"Institut Hospitalo\u2010Universitaire, P\u00f4le H\u00e9pato\u2010digestif, H\u00f4pitaux Universitaires de Strasbourg,Strasbourg,France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois\u2010Lo\u00efc","family":"Cosset","sequence":"additional","affiliation":[{"name":"CIRI\u2013International Center for Infectiology Research, Lyon, France; Inserm, U1111, Lyon France; Ecole Normale Sup\u00e9rieure; CNRS UMR 5308, Lyon, France; LabEx Ecofect,University of Lyon,Lyon,France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Berg","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Neurology and Dermatology, Gastroenterology and Rheumatology Clinic, Section of Hepatology,University of Leipzig,Leipzig,Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirjam B.","family":"Zeisel","sequence":"additional","affiliation":[{"name":"Inserm, U1110, Institut des Maladies Virales et H\u00e9patiques,Strasbourg,France"},{"name":"University of Strasbourg,Strasbourg,France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas F.","family":"Baumert","sequence":"additional","affiliation":[{"name":"Inserm, U1110, Institut des Maladies Virales et H\u00e9patiques,Strasbourg,France"},{"name":"University of Strasbourg,Strasbourg,France"},{"name":"Institut Hospitalo\u2010Universitaire, P\u00f4le H\u00e9pato\u2010digestif, H\u00f4pitaux Universitaires de Strasbourg,Strasbourg,France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2015,7,28]]},"reference":[{"key":"hep27906-bib-0001-20241017","first-page":"18","article-title":"Hepatitis C\u2014global prevalence (update)","volume":"75","year":"2000","journal-title":"Wkly Epidemiol Rec"},{"key":"hep27906-bib-0002-20241017","doi-asserted-by":"crossref","first-page":"29S","DOI":"10.1002\/hep.510260705","article-title":"Hepatitis C in asymptomatic blood donors","volume":"26","year":"1997","journal-title":"Hepatology"},{"key":"hep27906-bib-0003-20241017","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1084\/jem.191.9.1499","article-title":"Analysis of successful immune responses in persons infected with hepatitis C virus","volume":"191","year":"2000","journal-title":"J Exp Med"},{"key":"hep27906-bib-0004-20241017","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1084\/jem.194.10.1395","article-title":"Determinants of viral clearance and persistence during acute hepatitis C virus infection","volume":"194","year":"2001","journal-title":"J Exp Med"},{"key":"hep27906-bib-0005-20241017","doi-asserted-by":"crossref","first-page":"6023","DOI":"10.1128\/JVI.79.10.6023-6034.2005","article-title":"Human serum facilitates hepatitis C virus infection, and neutralizing responses inversely correlate with viral replication kinetics at the acute phase of hepatitis C virus infection","volume":"79","year":"2005","journal-title":"J Virol"},{"key":"hep27906-bib-0006-20241017","doi-asserted-by":"crossref","first-page":"6025","DOI":"10.1073\/pnas.0607026104","article-title":"Rapid induction of virus\u2010neutralizing antibodies and viral clearance in a single\u2010source outbreak of hepatitis C","volume":"104","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"hep27906-bib-0007-20241017","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1002\/hep.27013","article-title":"Clearance of hepatitis C infection is associated with the early appearance of broad neutralizing antibody responses","volume":"59","year":"2014","journal-title":"Hepatology"},{"key":"hep27906-bib-0008-20241017","doi-asserted-by":"crossref","first-page":"5007","DOI":"10.1128\/JVI.02286-07","article-title":"CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry","volume":"82","year":"2008","journal-title":"J Virol"},{"key":"hep27906-bib-0009-20241017","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/nm.2341","article-title":"EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy","volume":"17","year":"2011","journal-title":"Nat Med"},{"key":"hep27906-bib-0010-20241017","first-page":"302","article-title":"HRas signal transduction promotes hepatitis C virus cell entry by triggering assembly of the host tetraspanin receptor complex. Cell Host","volume":"13","year":"2013","journal-title":"Microbe"},{"key":"hep27906-bib-0011-20241017","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1053\/j.gastro.2010.05.073","article-title":"Monoclonal anti\u2010claudin 1 antibodies prevent hepatitis C virus infection of primary human hepatocytes","volume":"139","year":"2010","journal-title":"Gastroenterology"},{"key":"hep27906-bib-0012-20241017","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1002\/hep.26097","article-title":"The postbinding activity of scavenger receptor class B type I mediates initiation of hepatitis C virus infection and viral dissemination","volume":"57","year":"2013","journal-title":"Hepatology"},{"key":"hep27906-bib-0013-20241017","doi-asserted-by":"crossref","first-page":"e64221","DOI":"10.1371\/journal.pone.0064221","article-title":"A novel monoclonal anti\u2010CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell\u2010cell 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Hepatol"},{"key":"hep27906-bib-0017-20241017","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1023\/A:1008135413215","article-title":"Hepatitis C virus variability: sequence analysis of an isolate after 10 years of chronic infection","volume":"21","year":"2000","journal-title":"Virus Genes"},{"key":"hep27906-bib-0018-20241017","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/liv.12661","article-title":"Loss of hepatitis B surface antigen in a real\u2010life clinical cohort of patients with chronic hepatitis B virus infection","volume":"35","year":"2015","journal-title":"Liver Int"},{"key":"hep27906-bib-0019-20241017","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1002\/hep.23445","article-title":"Inhibition of hepatitis C virus infection by anti\u2010claudin\u20101 antibodies is mediated by neutralization of E2\u2010CD81\u2010claudin\u20101 associations","volume":"51","year":"2010","journal-title":"Hepatology"},{"key":"hep27906-bib-0020-20241017","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1002\/hep.21994","article-title":"Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81","volume":"46","year":"2007","journal-title":"Hepatology"},{"key":"hep27906-bib-0021-20241017","doi-asserted-by":"crossref","first-page":"11095","DOI":"10.1128\/JVI.79.17.11095-11104.2005","article-title":"Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoprotein","volume":"79","year":"2005","journal-title":"J Virol"},{"key":"hep27906-bib-0022-20241017","doi-asserted-by":"crossref","first-page":"10407","DOI":"10.1128\/JVI.74.22.10407-10416.2000","article-title":"Human monoclonal antibodies that inhibit binding of hepatitis C virus E2 protein to CD81 and recognize conserved conformational 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Immunol"},{"key":"hep27906-bib-0029-20241017","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1038\/nbt.3179","article-title":"Clearance of persistent hepatitis C virus infection using a monoclonal antibody specific for tight junction protein claudin\u20101","volume":"33","year":"2015","journal-title":"Nature Biotechnology"},{"key":"hep27906-bib-0030-20241017","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1097\/00005792-200001000-00005","article-title":"Extrahepatic manifestations associated with hepatitis C virus infection. A prospective multicenter study of 321 patients. The GERMIVIC. Groupe d'Etude et de Recherche en Medecine Interne et Maladies Infectieuses sur le Virus de l'Hepatite C","volume":"79","year":"2000","journal-title":"Medicine"},{"key":"hep27906-bib-0031-20241017","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1111\/jvh.12052","article-title":"Anti\u2010inflammatory cytokines, pro\u2010fibrogenic chemokines and persistence of acute HCV infection","volume":"20","year":"2013","journal-title":"J Viral Hepat"},{"key":"hep27906-bib-0032-20241017","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/jcla.2060","article-title":"Cytokine serum levels in patients with chronic HCV infection","volume":"16","year":"2002","journal-title":"J Clin Lab Anal"},{"key":"hep27906-bib-0033-20241017","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1002\/hep.21139","article-title":"Oxidized low\u2010density lipoprotein inhibits hepatitis C virus cell entry in human hepatoma cells","volume":"43","year":"2006","journal-title":"Hepatology"},{"key":"hep27906-bib-0034-20241017","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1007\/s00018-011-0872-6","article-title":"Polyreactive antibodies in adaptive immune responses to viruses","volume":"69","year":"2012","journal-title":"Cell Mol Life Sci"},{"key":"hep27906-bib-0035-20241017","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1038\/nature09385","article-title":"Polyreactivity increases the apparent affinity of anti\u2010HIV antibodies by heteroligation","volume":"467","year":"2010","journal-title":"Nature"},{"key":"hep27906-bib-0036-20241017","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0165-2427(03)00022-9","article-title":"Identification and characterization of auto\u2010anti\u2010idiotypic antibodies specific for antibodies against porcine reproductive and respiratory syndrome virus envelope glycoprotein (GP5)","volume":"92","year":"2003","journal-title":"Vet Immunol Immunopathol"},{"key":"hep27906-bib-0037-20241017","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1128\/jvi.62.5.1565-1572.1988","article-title":"Mice immunized with measles virus develop antibodies to a cell surface receptor for binding virus","volume":"62","year":"1988","journal-title":"J Virol"},{"key":"hep27906-bib-0038-20241017","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/1746-6148-10-39","article-title":"Anti\u2010idiotypic antibodies reduce efficacy of the attenuated vaccine against highly pathogenic PRRSV challenge","volume":"10","year":"2014","journal-title":"BMC Vet Res"},{"key":"hep27906-bib-0039-20241017","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1002\/hep.1840190407","article-title":"Immunological disorders in C virus chronic active hepatitis: a prospective case\u2010control study","volume":"19","year":"1994","journal-title":"Hepatology"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.27906","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-201509000-00012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T21:00:54Z","timestamp":1729198854000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-201509000-00012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,28]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.27906","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725514996.793507135","asserted-by":"object"}]},"ISSN":["0270-9139","1527-3350"],"issn-type":[{"value":"0270-9139","type":"print"},{"value":"1527-3350","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,28]]},"assertion":[{"value":"2014-12-02","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-19","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}