{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:35:54Z","timestamp":1791408954904,"version":"4.3.3"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>Autoimmune hepatitis (AIH) is known as a T cell\u2013mediated disease. However, AIH patients refractory to conventional treatment have been successfully treated with anti\u2010CD20\u2010mediated B\u2010cell depletion. The aim of this project was to understand the immunological changes underlying the AIH remission caused by B\u2010cell depletion in an experimental model of AIH. C57BL\/6 AIH mice, xenoimmunized with DNA coding for human liver antigens, were treated with a single dose of depleting mouse anti\u2010CD20 antibody at the peak of liver inflammation. Liver inflammation, alanine aminotransferase levels, chemokine (C\u2010X\u2010C) ligand 10 expression, and circulating B\u2010cell, autoantibody, and total immunoglobulin G levels were monitored following depletion. T\u2010cell and B\u2010cell phenotype and function were characterized. Administration of a single dose of anti\u2010CD20 resulted in a drastic reduction of liver inflammation accompanied by a significant reduction of alanine aminotransferase levels and of proinflammatory chemokine (C\u2010X\u2010C) ligand 10 expression. The treatment did not result in significant changes in total immunoglobulin G levels or autoantibodies. There were significantly more naive and less antigen\u2010experienced CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells, and T\u2010cell proliferation was significantly reduced following anti\u2010CD20 treatment. B cells served as antigen\u2010presenting cells to CD4<jats:sup>+<\/jats:sup> T cells. Anti\u2010CD20 treatment also led to a profound reduction of T follicular helper cells. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: B cells play an active role in the pathogenesis of AIH in antigen presentation processes and the modulation of T\u2010cell functions and influence the T follicular helper\u2013cell population; this active role of B cells could explain the success of B\u2010cell depletion for remission of AIH despite its classification as a T cell\u2013mediated autoimmune liver disease. (H<jats:sc>epatology<\/jats:sc> 2015;62:1511\u20131523)<\/jats:p>","DOI":"10.1002\/hep.27991","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T00:42:15Z","timestamp":1436920935000},"page":"1511-1523","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":88,"title":["Depletion of B cells induces remission of autoimmune hepatitis in mice through reduced antigen presentation and help to T cells"],"prefix":"10.1097","volume":"62","author":[{"given":"Kathie","family":"B\u00e9land","sequence":"first","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutrition,CHU Sainte\u2010Justine,Montreal,QC,Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Marceau","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology and Nutrition,CHU 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