{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:43:31Z","timestamp":1790898211214,"version":"4.1.0"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2017,10,30]],"date-time":"2017-10-30T00:00:00Z","timestamp":1509321600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,10,30]],"date-time":"2017-10-30T00:00:00Z","timestamp":1509321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p>Hepatitis delta virus (HDV) causes the most severe form of human viral hepatitis. HDV requires a hepatitis B virus (HBV) coinfection to provide HDV with HBV surface antigen envelope proteins. The net effect of HDV is to make the underlying HBV disease worse, including higher rates of hepatocellular carcinoma. Accurate assessments of current HDV prevalence have been hampered by the lack of readily available and reliable quantitative assays, combined with the absence of a Food and Drug Administration\u2013approved therapy. We sought to develop a convenient assay for accurately screening populations and to use this assay to determine HDV prevalence in a population with abnormally high rates of hepatocellular carcinoma. We developed a high\u2010throughput quantitative microarray antibody capture assay for anti\u2010HDV immunoglobulin G wherein recombinant HDV delta antigen is printed by microarray on slides coated with a noncontinuous, nanostructured plasmonic gold film, enabling quantitative fluorescent detection of anti\u2010HDV antibody in small aliquots of patient serum. This assay was then used to screen all HBV\u2010infected patients identified in a large randomly selected cohort designed to represent the Mongolian population. We identified two quantitative thresholds of captured antibody that were 100% predictive of the sample either being positive on standard western blot or harboring HDV RNA detectable by real\u2010time quantitative PCR. Subsequent screening of the HBV<jats:sup>+<\/jats:sup> cohort revealed that a remarkable 57% were RNA<jats:sup>+<\/jats:sup> and an additional 4% were positive on western blot alone. <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic> The quantitative microarray antibody capture assay's unique performance characteristics make it ideal for population screening; its application to the Mongolian HBV surface antigen\u2013positive population reveals an apparent \u223c60% prevalence of HDV coinfection among these HBV\u2010infected Mongolian subjects, which may help explain the extraordinarily high rate of hepatocellular carcinoma in Mongolia. (H<jats:sc>epatology<\/jats:sc> 2017;66:1739\u20131749)<\/jats:p>","DOI":"10.1002\/hep.28957","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T23:47:08Z","timestamp":1479944828000},"page":"1739-1749","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":106,"title":["A novel quantitative microarray antibody capture assay identifies an extremely high hepatitis delta virus prevalence among hepatitis B virus\u2013infected mongolians"],"prefix":"10.1097","volume":"66","author":[{"given":"Xiaohua","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology and Hepatology,Stanford University School of Medicine,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odgerel","family":"Oidovsambuu","sequence":"additional","affiliation":[{"name":"Liver Center,Ulaanbaatar,Mongolia"},{"name":"Mongolian National University,Ulaanbaatar,Mongolia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology and Hepatology,Stanford University School of Medicine,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosslyn","family":"Grosely","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology and Hepatology,Stanford University School of Medicine,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menashe","family":"Elazar","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology and Hepatology,Stanford University School of Medicine,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia D.","family":"Winn","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology,Stanford University,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Fram","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Gastroenterology and Hepatology,Stanford University School of Medicine,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang","family":"Boa","sequence":"additional","affiliation":[{"name":"Department of Chemistry,Stanford University,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Chemistry,Stanford University,Palo Alto,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bekhbold","family":"Dashtseren","sequence":"additional","affiliation":[{"name":"Liver Center,Ulaanbaatar,Mongolia"},{"name":"Onom Foundation,Ulaanbaatar,Mongolia"},{"name":"Mongolian National University of Health Sciences,Ulaanbaatar,Mongolia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahgwahdorj","family":"Yagaanbuyant","sequence":"additional","affiliation":[{"name":"Liver Center,Ulaanbaatar,Mongolia"},{"name":"Onom Foundation,Ulaanbaatar,Mongolia"},{"name":"Mongolian National University of Health Sciences,Ulaanbaatar,Mongolia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zulkhuu","family":"Genden","sequence":"additional","affiliation":[{"name":"Liver Center,Ulaanbaatar,Mongolia"},{"name":"Onom Foundation,Ulaanbaatar,Mongolia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naranbaatar","family":"Dashdorj","sequence":"additional","affiliation":[{"name":"Onom Foundation,Ulaanbaatar,Mongolia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Bungert","sequence":"additional","affiliation":[{"name":"Onom 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