{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:16:12Z","timestamp":1790745372949,"version":"4.1.0"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:00:00Z","timestamp":1498089600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:00:00Z","timestamp":1498089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:p>The diagnosis of alcoholic hepatitis (AH) often requires a transjugular liver biopsy (TJLB), a procedure that is not always readily accessible. We analyzed plasma biomarkers to estimate the presence of histological features of AH among patients with clinical suspicion of AH. Using enzyme\u2010linked immunosorbent assay, we tested M65 and M30 (circulating fragments of cytokeratin\u201018) and their respective fraction carried by microvesicles (MVs), CCL20 and TREM1. Leukocyte, platelet, and endothelial\u2010derived MVs were quantified by way of flow cytometry. Test and validation cohorts prospectively included patients with clinical features of AH undergoing TJLB. In the test cohort, 46 of 83 (55%) patients showed histological features of AH. Age, bilirubin, INR, and creatinine (ABIC) score was B or C in 83%. Patients with histologically proven AH had higher levels of total and MV\u2010bound M65 and total and MV\u2010bound M30 and CCL20 than those without (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.001 for all tests). Levels of TREM\u20101 and of subpopulations of MVs were not different between groups. M65 and M30 both had an area under the receiver operating characteristics curve of 0.84 to estimate the presence of AH. For M65, a cutoff of 2000 IU\/L had a positive predictive value of 91%, whereas a cutoff of 641 IU\/L had a negative predictive value of 88%. In the validation cohort, AH was histologically confirmed in 48 of 68 (71%) patients. ABIC score was B or C in 69% of patients. For M65, the above cutoffs had a diagnostic accuracy of 81%. Even better results were obtained in patients with suspicion of severe AH (ABIC B or C) in both cohorts. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: Plasma levels of cytokeratin\u201018 fragments are reliable noninvasive markers of AH. Using the proposed cutoffs for M65, two thirds of TJLB can be avoided, which can be useful in centers where this technique is not readily available. (H<jats:sc>epatology<\/jats:sc> 2017;66:555\u2013563).<\/jats:p>","DOI":"10.1002\/hep.29080","type":"journal-article","created":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T04:06:40Z","timestamp":1485317200000},"page":"555-563","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":109,"title":["A prospective study of the utility of plasma biomarkers to diagnose alcoholic hepatitis"],"prefix":"10.1097","volume":"66","author":[{"given":"Julien","family":"Bissonnette","sequence":"first","affiliation":[{"name":"Service d'H\u00e9patologie,DHU Unity, H\u00f4pital Beaujon, Assistance Publique\u2010H\u00f4pitaux de Paris,Clichy,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Altamirano","sequence":"additional","affiliation":[{"name":"Liver Unit\u2010Internal Medicine Department,Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Vall d'Hebron University Hospital, Vall d'Hebron Institut de Recerca (VHIR),Barcelona,Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Devue","sequence":"additional","affiliation":[{"name":"INSERM,U970, Paris Cardiovascular Research Center\u2013PARCC,Paris,France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9,UMR\u2010S970,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Roux","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9patologie,DHU Unity, H\u00f4pital Beaujon, Assistance Publique\u2010H\u00f4pitaux de Paris,Clichy,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Payanc\u00e9","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9patologie,DHU Unity, H\u00f4pital Beaujon, Assistance Publique\u2010H\u00f4pitaux de 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Publique\u2010H\u00f4pitaux de Paris,Clichy,France"},{"name":"INSERM,UMR1149, Centre de Recherche sur l'Inflammation,Paris,France"},{"name":"Universit\u00e9 Denis Diderot\u2010Paris 7,Sorbonne Paris Cit\u00e9,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Durand","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9patologie,DHU Unity, H\u00f4pital Beaujon, Assistance Publique\u2010H\u00f4pitaux de Paris,Clichy,France"},{"name":"INSERM,UMR1149, Centre de Recherche sur l'Inflammation,Paris,France"},{"name":"Universit\u00e9 Denis Diderot\u2010Paris 7,Sorbonne Paris Cit\u00e9,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hafid","family":"Ait\u2010Oufella","sequence":"additional","affiliation":[{"name":"INSERM,U970, Paris Cardiovascular Research Center\u2013PARCC,Paris,France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9,UMR\u2010S970,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pau","family":"Sancho\u2010Bru","sequence":"additional","affiliation":[{"name":"Liver Unit,Hospital Clinic, Faculty of Medicine, University of Barcelona,Barcelona,Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan","family":"Caballeria","sequence":"additional","affiliation":[{"name":"Liver Unit,Hospital Clinic, Faculty of Medicine, University of Barcelona,Barcelona,Spain"},{"name":"Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer,(IDIBAPS), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd),Barcelona,Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pere","family":"Gin\u00e8s","sequence":"additional","affiliation":[{"name":"Liver Unit,Hospital Clinic, Faculty of Medicine, University of Barcelona,Barcelona,Spain"},{"name":"Institut d'Investigacions Biom\u00e8diques August Pi 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