{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T13:55:51Z","timestamp":1793973351916,"version":"4.8.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2017,4,28]],"date-time":"2017-04-28T00:00:00Z","timestamp":1493337600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,4,28]],"date-time":"2017-04-28T00:00:00Z","timestamp":1493337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>There is a need for further refinement of current histological systems for assessment of hepatic fibrosis in nonalcoholic fatty liver disease (NAFLD). This study evaluated hepatic fibrosis in NAFLD using dual\u2010photon microscopy\u2010based quantitation of fibrosis\u2010related parameters (q\u2010FPs). Fifty test cohort subjects and 42 validation cohort subjects with NAFLD and the full spectrum of fibrosis were studied. q\u2010FPs were measured in specific predefined regions of interest (general, vessel, perisinusoid, and vascular septa). Seventy q\u2010FPs had inter\u2010 and intraobserver concordance \u22650.8 and were related to the NASH Clinical Research Network fibrosis staging. Of these, 16 q\u2010FPs with the strongest correlations (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.001 for all) were entered in a principal component analysis model (odds ratio [OR] 7.8, <jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.001), which separated any stage of fibrosis versus no fibrosis, and cirrhosis versus earlier stages with the areas under the receiver operating characteristic curves of 0.88 and 0.93 (<jats:italic toggle=\"yes\">P<\/jats:italic> \u2264 0.01 for both), respectively. In an independent multivariable analysis, four q\u2010FPs\u2014the number of collagen strands (OR 8.5, <jats:italic toggle=\"yes\">P<\/jats:italic> = 0.004), strand length (OR 12.0, <jats:italic toggle=\"yes\">P<\/jats:italic> = 0.02), strand eccentricity (OR 8.3, <jats:italic toggle=\"yes\">P<\/jats:italic> = 0.004), and strand solidity (OR 8.0, <jats:italic toggle=\"yes\">P<\/jats:italic> = 0.003)\u2014were independently associated with fibrosis stages and were used to model fibrosis along a continuous linear scale using desirability functions; this linear scale of fibrosis measurement was also related to fibrosis stage (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; 0.0001). The robustness of both the multivariable model and the linear scale of measurement was confirmed in the validation cohort. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: The q\u2010FP model provides an accurate reproducible method to evaluate fibrosis in NAFLD along a quantitative and continuous scale. (H<jats:sc>epatology<\/jats:sc> 2017;65:1891\u20101903).<\/jats:p>","DOI":"10.1002\/hep.29090","type":"journal-article","created":{"date-parts":[[2017,1,30]],"date-time":"2017-01-30T09:15:10Z","timestamp":1485767710000},"page":"1891-1903","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":54,"title":["Dual\u2010photon microscopy\u2010based quantitation of fibrosis\u2010related parameters (q\u2010FP) to model disease progression in steatohepatitis"],"prefix":"10.1097","volume":"65","author":[{"given":"Yan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases & Hepatology Unit,Southern Medical University Nanfang Hospital,GZ China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Vincent","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlian","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases & Hepatology Unit,Southern Medical University Nanfang Hospital,GZ China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amon","family":"Asgharpour","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xieer","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases & Hepatology Unit,Southern Medical University Nanfang Hospital,GZ China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael O.","family":"Idowu","sequence":"additional","affiliation":[{"name":"Divsion of Surgical Pathology, Department of Pathology,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa J.","family":"Contos","sequence":"additional","affiliation":[{"name":"Divsion of Surgical Pathology, Department of Pathology,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyani","family":"Daitya","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed S.","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faridoddin","family":"Mirshahi","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun J.","family":"Sanyal","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine,Virginia Commonwealth University School of Medicine,Richmond,VA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2017,4,28]]},"reference":[{"key":"hep29090-bib-0001-20241017","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1053\/j.gastro.2011.06.061","article-title":"Frequency and outcomes of liver transplantation for nonalcoholic steatohepatitis in the United 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