{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:39:24Z","timestamp":1760985564729,"version":"3.41.2"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2017,8,26]],"date-time":"2017-08-26T00:00:00Z","timestamp":1503705600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2017,8,26]],"date-time":"2017-08-26T00:00:00Z","timestamp":1503705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p>The development of pathogenic mechanisms, specific antiviral treatments and preventive vaccines for hepatitis C virus (HCV) infection has been limited due to lack of cell culture models that can naturally imitate the entire HCV life cycle. Here, we established an HCV cell culture model based on human fetal liver stem cells (hFLSCs) that supports the entire blood\u2010borne hepatitis C virus (bbHCV) life cycle. More than 90% of cells remained infected by various genotypes. bbHCV was efficiently propagated, and progeny virus were infectious to hFLSCs. The virus could be passed efficiently between cells. The viral infectivity was partially blocked by specific antibodies or small interfering RNA against HCV entry factors, whereas HCV replication was inhibited by antiviral drugs. We observed viral particles of approximately 55 nm in diameter in both cell culture media and infected cells after bbHCV infection. <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic> Our data show that the entire bbHCV life cycle could be naturally imitated in hFLSCs. This model is expected to provide a powerful tool for exploring the process and the mechanism of bbHCV infection at the cellular level and for evaluating the treatment and preventive strategies of bbHCV infection. (H<jats:sc>epatology<\/jats:sc> 2017;66:1045\u20101057).<\/jats:p>","DOI":"10.1002\/hep.29211","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T14:33:59Z","timestamp":1492094039000},"page":"1045-1057","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Efficient replication of blood\u2010borne hepatitis C virus in human fetal liver stem cells"],"prefix":"10.1097","volume":"66","author":[{"given":"Xuan","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory 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Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Qiang","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao\u2010Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Fei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Lian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Environment and Health,Tianjin Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety,Tianjin,China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2017,8,26]]},"reference":[{"key":"hep29211-bib-0001-20250714","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1038\/nm.3248","article-title":"Understanding the hepatitis C virus life cycle paves the way for highly effective therapies","volume":"19","year":"2013","journal-title":"Nat Med"},{"key":"hep29211-bib-0002-20250714","first-page":"5","article-title":"Understanding the molecular targets for new therapeutical agents in hepatitis c infection","volume":"72","year":"2013","journal-title":"Roum Arch Microbiol 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