{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:41:14Z","timestamp":1792269674780,"version":"4.4.0"},"reference-count":49,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:p>Hepatitis B virus (HBV) can manipulate the microRNA (miRNA) regulatory networks in infected cells to create a permissive environment for viral replication, cellular injury, disease onset, and its progression. The aim of the present study was to understand the miRNA networks and their target genes in the liver of hepatitis B patients involved in HBV replication, liver injury, and liver fibrosis. We investigated differentially expressed miRNAs by microarray in liver biopsy samples from different stages of HBV infection and liver disease (immune\u2010tolerant [n\u2009=\u20098], acute viral hepatitis [n\u2009=\u20098], no fibrosis [n\u2009=\u200916], early [F1+F2, n\u2009=\u200919] or late [F3+F4, n\u2009=\u200914] fibrosis, and healthy controls [n\u2009=\u20097]). miRNA expression levels were analyzed by unsupervised principal component analysis and hierarchical clustering. Analysis of miRNA\u2013mRNA regulatory networks identified 17 miRNAs and 18 target gene interactions with four distinct nodes, each representing a stage\u2010specific gene regulation during disease progression. The immune\u2010tolerant group showed elevated miR\u2010199a\u20105p, miR\u2010221\u20103p, and Let\u20107a\u20103p levels, which could target genes involved in innate immune response and viral replication. In the acute viral hepatitis group, miR\u2010125b\u20105p and miR\u20103613\u20103p were up, whereas miR\u2010940 was down, which might affect cell proliferation through the signal transducer and activator of transcription 3 pathway. In early fibrosis, miR\u201034b\u20103p, miR\u20101224\u20103p, and miR\u20101227\u20103p were up, while miR\u2010499a\u20105p was down, which together possibly mediate chronic inflammation. In advanced fibrosis, miR\u20101, miR\u201010b\u20105p, miR\u201096\u20105p, miR\u2010133b, and miR\u2010671\u20105p were up, while miR\u201020b\u20105p and miR\u2010455\u20103p were down, possibly allowing chronic disease progression. Interestingly, only 8 of 17 liver\u2010specific miRNAs exhibited a similar expression pattern in patient sera. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: miRNA signatures identified in this study corroborate previous findings and provide fresh insight into the understanding of HBV\u2010associated liver diseases which may be helpful in developing early\u2010stage disease diagnostics and targeted therapeutics. (H<jats:sc>epatology<\/jats:sc> 2018;67:1695\u20101709)<\/jats:p>","DOI":"10.1002\/hep.29690","type":"journal-article","created":{"date-parts":[[2017,11,30]],"date-time":"2017-11-30T18:22:43Z","timestamp":1512066163000},"page":"1695-1709","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":79,"title":["Global microRNA expression profiling in the liver biopsies of hepatitis B virus\u2013infected patients suggests specific microRNA signatures for viral persistence and hepatocellular injury"],"prefix":"10.1097","volume":"67","author":[{"given":"Avishek Kumar","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheetalnath Babasaheb","family":"Rooge","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of 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Asian University,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirupama","family":"Trehanpati","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Hepatology,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Geffers","sequence":"additional","affiliation":[{"name":"Genome Analytics, Helmholtz Centre for Infection Research,Braunschweig,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New 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