{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:01:02Z","timestamp":1790719262389,"version":"4.1.0"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T00:00:00Z","timestamp":1526860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T00:00:00Z","timestamp":1526860800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:p>Microvesicles (MVs) are extracellular vesicles released by cells following activation or apoptosis. Some MV subpopulations augment with cirrhosis severity and contribute to portal hypertension. This study aimed at determining if plasma MV levels can estimate the presence of hepatic venous pressure gradient (HVPG) \u226510 mm Hg and predict mortality in patients with advanced chronic liver disease. All patients with severe fibrosis or cirrhosis undergoing liver catheterization between 2013 and 2015 at two centers were prospectively included. We measured circulating levels of annexin V<jats:sup>+<\/jats:sup>, platelet, leukocyte, endothelial, and hepatocyte MVs. The test cohort included 139 patients. Hepatocyte MV levels were 4.0\u2010fold and 2.2\u2010fold higher in patients with Child\u2010Pugh C than in those with Child\u2010Pugh A or B liver disease, respectively. Levels of other MV subpopulations were not influenced by liver disease severity. Hepatocyte MV levels correlated with HVPG but could not identify patients with HVPG \u226510 mm Hg. Hepatocyte MV level &gt;65 U\/L predicted 6\u2010month mortality independently of Child\u2010Pugh score and of Model for End\u2010Stage Liver Disease (MELD). Patients with hepatocyte MV levels &gt;65 U\/L and MELD &gt;15 had a higher 6\u2010month mortality than other patients (23% versus 3%; <jats:italic toggle=\"yes\">P<\/jats:italic> = 0.001). These findings were confirmed in a validation cohort including 103 patients. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: Circulating MV levels cannot identify patients with HVPG \u226510 mm Hg; by contrast, hepatocyte MV levels strongly improve prediction of 6\u2010month mortality in patients with advanced chronic liver disease; therapies associated with decreased levels of circulating hepatocyte MV might be attractive strategies in patients with severe cirrhosis. (H<jats:sc>epatology<\/jats:sc> 2018).<\/jats:p>","DOI":"10.1002\/hep.29903","type":"journal-article","created":{"date-parts":[[2018,3,31]],"date-time":"2018-03-31T01:55:54Z","timestamp":1522461354000},"page":"1508-1518","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":51,"title":["Hepatocyte microvesicle levels improve prediction of mortality in patients with cirrhosis"],"prefix":"10.1097","volume":"68","author":[{"given":"Audrey","family":"Payanc\u00e9","sequence":"first","affiliation":[{"name":"DHU Unity, P\u00f4le des Maladies de l'Appareil Digestif, Service d'H\u00e9patologie, Centre de R\u00e9f\u00e9rence des Maladies Vasculaires du Foie,H\u00f4pital Beaujon, AP\u2010HP,Clichy,France"},{"name":"Universit\u00e9 Denis Diderot\u2010Paris 7, Sorbonne Paris Cit\u00e9,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7002-4134","authenticated-orcid":false,"given":"Gilberto","family":"Silva\u2010Junior","sequence":"additional","affiliation":[{"name":"Hepatic Hemodynamic Laboratory, Liver Unit,Hospital Clinic, IDIBAPS and CIBERehd,Barcelona,Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Bissonnette","sequence":"additional","affiliation":[{"name":"DHU Unity, P\u00f4le des Maladies de l'Appareil Digestif, Service d'H\u00e9patologie, Centre de R\u00e9f\u00e9rence des Maladies Vasculaires du Foie,H\u00f4pital Beaujon, AP\u2010HP,Clichy,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Tanguy","sequence":"additional","affiliation":[{"name":"Inserm, U970,Paris Cardiovascular Research Center\u2013PARCC,Paris,France"},{"name":"Universit\u00e9 Paris Descartes, Sorbonne Paris 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AP\u2010HP,Clichy,France"},{"name":"Universit\u00e9 Denis Diderot\u2010Paris 7, Sorbonne Paris Cit\u00e9,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ouardia","family":"Nekachtali","sequence":"additional","affiliation":[{"name":"DHU Unity, P\u00f4le des Maladies de l'Appareil Digestif, Service d'H\u00e9patologie, Centre de R\u00e9f\u00e9rence des Maladies Vasculaires du Foie,H\u00f4pital Beaujon, AP\u2010HP,Clichy,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Baiges","sequence":"additional","affiliation":[{"name":"Hepatic Hemodynamic Laboratory, Liver Unit,Hospital Clinic, IDIBAPS and CIBERehd,Barcelona,Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Hern\u00e1ndez\u2010Gea","sequence":"additional","affiliation":[{"name":"Hepatic Hemodynamic Laboratory, Liver Unit,Hospital Clinic, IDIBAPS and CIBERehd,Barcelona,Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9dric","family":"Laou\u00e9nan","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de recherche clinique Paris Nord,H\u00f4pital Bichat, AP\u2010HP,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Lebrec","sequence":"additional","affiliation":[{"name":"DHU Unity, P\u00f4le des Maladies de l'Appareil Digestif, Service d'H\u00e9patologie, Centre de R\u00e9f\u00e9rence des Maladies Vasculaires du Foie,H\u00f4pital Beaujon, AP\u2010HP,Clichy,France"},{"name":"Universit\u00e9 Denis Diderot\u2010Paris 7, Sorbonne Paris Cit\u00e9,Paris,France"},{"name":"Inserm, U1149,Centre de Recherche sur l'Inflammation,Paris,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Inserm, U1149,Centre de Recherche sur l'Inflammation,Paris,France"},{"name":"DHU 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