{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T13:56:43Z","timestamp":1793973403851,"version":"4.8.0"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,5]]},"DOI":"10.1002\/hep.30425","type":"journal-article","created":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T08:54:38Z","timestamp":1544777678000},"page":"2214-2231","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":38,"title":["Pharmacological Induction of a Progenitor State for the Efficient Expansion of Primary Human Hepatocytes"],"prefix":"10.1097","volume":"69","author":[{"given":"Carmen","family":"Unzu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evarist","family":"Planet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Brandenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Floriane","family":"Fusil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Cassano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimena","family":"Perez\u2010Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Friedli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois\u2010Lo\u00efc","family":"Cosset","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias P.","family":"Lutolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara E.","family":"Wildhaber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Trono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-33-20241017","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1172\/JCI66027","article-title":"Hepatic stem cell niches","volume":"123","author":"Kordes","year":"2013","journal-title":"J Clin Invest"},{"key":"R2-33-20241017","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/nature14863","article-title":"Self\u2010renewing diploid Axin2+ cells fuel homeostatic renewal of the liver","volume":"524","author":"Wang","year":"2015","journal-title":"Nature"},{"key":"R3-33-20241017","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1053\/jlts.2001.21699","article-title":"How can we optimize the outcome of liver grafts from pediatric donors?","volume":"7","author":"McDiarmid","year":"2001","journal-title":"Liver Transpl"},{"key":"R4-33-20241017","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1046\/j.1365-2613.1998.00083.x","article-title":"The current status of primary hepatocyte culture","volume":"79","author":"Mitaka","year":"1998","journal-title":"Int J Exp Pathol"},{"key":"R5-33-20241017","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors","volume":"126","author":"Takahashi","year":"2006","journal-title":"Cell"},{"key":"R6-33-20241017","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.stem.2013.06.017","article-title":"Reprogramming fibroblasts into bipotential hepatic stem cells by defined factors","volume":"13","author":"Yu","year":"2013","journal-title":"Cell Stem Cell"},{"key":"R7-33-20241017","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/nature13020","article-title":"Mouse liver repopulation with hepatocytes generated from human fibroblasts","volume":"508","author":"Zhu","year":"2014","journal-title":"Nature"},{"key":"R8-33-20241017","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/hep.23354","article-title":"Highly efficient generation of human hepatocyte\u2010like cells from induced pluripotent stem cells","volume":"51","author":"Si\u2010Tayeb","year":"2010","journal-title":"Hepatology"},{"key":"R9-33-20241017","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.stem.2013.07.001","article-title":"Genetic and epigenetic variations in iPSCs: potential causes and implications for application","volume":"13","author":"Liang","year":"2013","journal-title":"Cell Stem Cell"},{"key":"R10-33-20241017","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/nature13551","article-title":"Abnormalities in human pluripotent cells due to reprogramming mechanisms","volume":"511","author":"Ma","year":"2014","journal-title":"Nature"},{"key":"R11-33-20241017","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1101\/gr.172809.114","article-title":"Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency","volume":"24","author":"Friedli","year":"2014","journal-title":"Genome Res"},{"key":"R12-33-20241017","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.cell.2014.11.050","article-title":"Long\u2010term culture of genome\u2010stable bipotent stem cells from adult human liver","volume":"160","author":"Huch","year":"2015","journal-title":"Cell"},{"key":"R13-33-20241017","doi-asserted-by":"crossref","first-page":"717","DOI":"10.3727\/000000007783465154","article-title":"Adult\u2010derived human liver mesenchymal\u2010like cells as a potential progenitor reservoir of hepatocytes?","volume":"16","author":"Najimi","year":"2007","journal-title":"Cell Transplant"},{"key":"R14-33-20241017","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.stem.2014.04.010","article-title":"Stem\/progenitor cells in liver development, homeostasis, regeneration, and reprogramming","volume":"14","author":"Miyajima","year":"2014","journal-title":"Cell Stem Cell"},{"key":"R15-33-20241017","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1038\/nbt.3377","article-title":"Long\u2010term culture and expansion of primary human hepatocytes","volume":"33","author":"Levy","year":"2015","journal-title":"Nat Biotechnol"},{"key":"R16-33-20241017","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1002\/hep.21973","article-title":"Wnt\/beta\u2010catenin signaling mediates oval cell response in rodents","volume":"47","author":"Apte","year":"2008","journal-title":"Hepatology"},{"key":"R17-33-20241017","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1038\/nm.2667","article-title":"Macrophage\u2010derived Wnt opposes Notch signaling to specify hepatic progenitor cell fate in chronic liver disease","volume":"18","author":"Boulter","year":"2012","journal-title":"Nat Med"},{"key":"R18-33-20241017","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1101\/gad.268961.115","article-title":"FGF2 mediates hepatic progenitor cell formation during human pluripotent stem cell differentiation by inducing the WNT antagonist NKD1","volume":"29","author":"Twaroski","year":"2015","journal-title":"Genes Dev"},{"key":"R20-33-20241017","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/stem.576","article-title":"An endothelial cell niche induces hepatic specification through dual repression of Wnt and Notch signaling","volume":"29","author":"Han","year":"2011","journal-title":"Stem Cells"},{"key":"R21-33-20241017","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1002\/emmm.201000085","article-title":"Regulation of liver regeneration by growth factors and cytokines","volume":"2","author":"Bohm","year":"2010","journal-title":"EMBO Mol Med"},{"key":"R22-33-20241017","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1097\/TP.0b013e31819ca245","article-title":"A step toward liver gene therapy: efficient correction of the genetic defect of hepatocytes isolated from a patient with Crigler\u2010Najjar syndrome type 1 with lentiviral vectors","volume":"87","author":"Birraux","year":"2009","journal-title":"Transplantation"},{"key":"R23-33-20241017","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1126\/science.1239278","article-title":"Pluripotent stem cells induced from mouse somatic cells by small\u2010molecule compounds","volume":"341","author":"Hou","year":"2013","journal-title":"Science"},{"key":"R24-33-20241017","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1016\/0016-5085(92)90774-S","article-title":"Mitotic responsiveness of cultured adult human hepatocytes to epidermal growth factor, transforming growth factor alpha, and human serum","volume":"102","author":"Blanc","year":"1992","journal-title":"Gastroenterology"},{"key":"R25-33-20241017","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1002\/hep.26511","article-title":"Cross\u2010talk between Notch and Hedgehog regulates hepatic stellate cell fate in mice","volume":"58","author":"Xie","year":"2013","journal-title":"Hepatology"},{"key":"R26-33-20241017","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.stem.2016.06.011","article-title":"Molecular criteria for defining the naive human pluripotent state","volume":"19","author":"Theunissen","year":"2016","journal-title":"Cell Stem Cell"},{"key":"R27-33-20241017","doi-asserted-by":"crossref","first-page":"20569","DOI":"10.1073\/pnas.1319061110","article-title":"Differentiation\u2010defective phenotypes revealed by large\u2010scale analyses of human pluripotent stem cells","volume":"110","author":"Koyanagi\u2010Aoi","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"R28-33-20241017","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1038\/nsmb.2799","article-title":"The retrovirus HERVH is a long noncoding RNA required for human embryonic stem cell identity","volume":"21","author":"Lu","year":"2014","journal-title":"Nat Struct Mol Biol"},{"key":"R29-33-20241017","doi-asserted-by":"crossref","first-page":"E261","DOI":"10.3390\/v8090261","article-title":"Cell culture models for the investigation of hepatitis B and D virus infection","volume":"8","author":"Verrier","year":"2016","journal-title":"Viruses"},{"key":"R30-33-20241017","doi-asserted-by":"crossref","first-page":"e00049","DOI":"10.7554\/eLife.00049","article-title":"Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus","volume":"1","author":"Yan","year":"2012","journal-title":"Elife"},{"key":"R31-33-20241017","doi-asserted-by":"crossref","first-page":"12193","DOI":"10.1073\/pnas.1412631111","article-title":"Modeling host interactions with hepatitis B virus using primary and induced pluripotent stem cell\u2013derived hepatocellular systems","volume":"111","author":"Shlomai","year":"2014","journal-title":"Proc Natl Acad Sci USA"},{"key":"R32-33-20241017","doi-asserted-by":"crossref","first-page":"8087","DOI":"10.1016\/j.biomaterials.2011.07.028","article-title":"Concave microwell based size\u2010controllable hepatosphere as a three\u2010dimensional liver tissue model","volume":"32","author":"Wong","year":"2011","journal-title":"Biomaterials"},{"key":"R33-33-20241017","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1016\/j.biomaterials.2008.03.016","article-title":"Three\u2010dimensional extracellular matrix\u2013directed cardioprogenitor differentiation: systematic modulation of a synthetic cell\u2010responsive PEG\u2010hydrogel","volume":"29","author":"Kraehenbuehl","year":"2008","journal-title":"Biomaterials"},{"key":"R34-33-20241017","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1007\/s00204-013-1078-5","article-title":"Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non\u2010parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME","volume":"87","author":"Godoy","year":"2013","journal-title":"Arch Toxicol"},{"key":"R35-33-20241017","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/cr.2012.144","article-title":"Promotion of the efficient metabolic maturation of human pluripotent stem cell\u2013derived hepatocytes by correcting specification defects","volume":"23","author":"Zhao","year":"2013","journal-title":"Cell Res"},{"key":"R36-33-20241017","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.semcdb.2013.01.003","article-title":"Aneuploidy, polyploidy and ploidy reversal in the liver","volume":"24","author":"Duncan","year":"2013","journal-title":"Semin Cell Dev Biol"},{"key":"R37-33-20241017","doi-asserted-by":"crossref","first-page":"G881","DOI":"10.1152\/ajpgi.00289.2013","article-title":"Evidence for and against epithelial\u2010to\u2010mesenchymal transition in the liver","volume":"305","author":"Xie","year":"2013","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"R38-33-20241017","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.stem.2016.10.007","article-title":"Conversion of terminally committed hepatocytes to culturable bipotent progenitor cells with regenerative capacity","volume":"20","author":"Katsuda","year":"2017","journal-title":"Cell Stem Cell"},{"key":"R39-33-20241017","doi-asserted-by":"crossref","first-page":"eaah5505","DOI":"10.1126\/scitranslmed.aah5505","article-title":"In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease","volume":"9","author":"Stevens","year":"2017","journal-title":"Sci Transl Med"},{"key":"R40-33-20241017","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nmat4536","article-title":"Defined three\u2010dimensional microenvironments boost induction of pluripotency","volume":"15","author":"Caiazzo","year":"2016","journal-title":"Nat Mater"},{"key":"R41-33-20241017","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1002\/jbm.a.34764","article-title":"Decellularized liver scaffolds effectively support the proliferation and differentiation of mouse fetal hepatic progenitors","volume":"102","author":"Wang","year":"2014","journal-title":"J Biomed Mater Res A"},{"key":"R42-33-20241017","doi-asserted-by":"crossref","first-page":"14003","DOI":"10.1038\/mtm.2014.3","article-title":"Human fetal liver cells for regulated ex vivo erythropoietin gene therapy","volume":"1","author":"Filali","year":"2014","journal-title":"Mol Ther Methods Clin Dev"},{"key":"R43-33-20241017","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1002\/stem.2368","article-title":"Concise review: advances in generating hepatocytes from pluripotent stem cells for translational medicine","volume":"34","author":"Szkolnicka","year":"2016","journal-title":"Stem Cells"},{"key":"R44-33-20241017","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1038\/nprot.2015.128","article-title":"Micropatterned coculture of primary human hepatocytes and supportive cells for the study of hepatotropic pathogens","volume":"10","author":"March","year":"2015","journal-title":"Nat Protoc"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.30425","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hep.30425","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hep.30425","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-201905000-00033","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T19:16:29Z","timestamp":1729192589000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-201905000-00033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.30425","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.734634975.793554428","asserted-by":"object"}]},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[2019,5]]}}}