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We examined Wnt gene expression and signaling after injury, and we show by\n                    <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                    hybridization that Wnts are activated by acute carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    ) toxicity. Following injury, peri\u2010injury hepatocytes become Wnt\u2010responsive, expressing the Wnt target gene axis inhibition protein 2 (Axin2). Lineage tracing of peri\u2010injury Axin2\n                    <jats:sup>+<\/jats:sup>\n                    hepatocytes shows that during recovery the injured parenchyma becomes repopulated and repaired by Axin2\n                    <jats:sup>+<\/jats:sup>\n                    descendants. Using single\u2010cell RNA sequencing, we show that endothelial cells are the major source of Wnts following acute CCl\n                    <jats:sub>4<\/jats:sub>\n                    toxicity. Induced loss of \u03b2\u2010catenin in peri\u2010injury hepatocytes results in delayed repair and ultimately injury\u2010induced lethality, while loss of Wnt production from endothelial cells leads to a delay in the proliferative response after injury.\n                    <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic>\n                    Our findings highlight the importance of the Wnt\/\u03b2\u2010catenin signaling pathway in restoring tissue integrity following acute liver toxicity and establish a role of endothelial cells as an important Wnt\u2010producing regulator of liver tissue repair following localized liver injury.\n                  <\/jats:p>","DOI":"10.1002\/hep.30563","type":"journal-article","created":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T09:03:14Z","timestamp":1550134994000},"page":"2623-2635","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":109,"title":["Tissue Repair in the Mouse Liver Following Acute Carbon Tetrachloride Depends on Injury\u2010Induced Wnt\/\u03b2\u2010Catenin Signaling"],"prefix":"10.1097","volume":"69","author":[{"given":"Ludan","family":"Zhao","sequence":"first","affiliation":[{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Department of Developmental Biology,Howard Hughes Medical Institute, Stanford School of Medicine,Stanford,CA"},{"name":"Medical Scientist Training Program, Stanford School of Medicine,Stanford,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhua","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Department of Developmental Biology,Howard Hughes Medical Institute, Stanford School of Medicine,Stanford,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Donahue","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Department of Developmental Biology,Howard Hughes Medical Institute, Stanford School of Medicine,Stanford,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Tsui","sequence":"additional","affiliation":[{"name":"Department of Medicine and Liver Center,University of California San Francisco,San Francisco,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Fish","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Department of Developmental Biology,Howard Hughes Medical Institute, Stanford School of Medicine,Stanford,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catriona Y.","family":"Logan","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Department of Developmental Biology,Howard Hughes Medical Institute, Stanford School of 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