{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T10:55:14Z","timestamp":1760266514470,"version":"3.30.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":6848,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>\n                                 <jats:bold>Antibodies against the 52\u2013kd Ro(SS\u2013A) protein are significantly associated with the primary Sjogren's syndrome (pSS). A small proportion of patients suffering from primary biliary cirrhosis (PBC) with secondary Sjogren's syndrome (PBC\/SS) who are serologically characterized by antimitochondrial type 2 antibodies also express anti\u201352\u2013kd Ro(SS\u2013A) antibodies. The primary B\u2013cell\u2013derived antigenic responses by autoimmune sera were analyzed in both entities using truncated recombinant proteins to examine whether different epitopes are associated with these diseases. Sera were collected from 25 patients with pSS and 9 anti\u201352\u2013kd Ro(SS\u2013A)\u2013positive patients suffering from PBC\/SS. B\u2013cell epitope mapping was performed using different 52\u2013kd Ro(SS\u2013A) fusion proteins in enzyme\u2013linked immunosorbent assay (ELISA) and immunoblotting. Sera from patients with pSS showed the broadest reactivity against antigenic epitopes at AA 153\u2013245 compared to the significantly limited reactivity against AA 228\u2013245 of sera from patients with PBC. B\u2013cell epitopes within AA 190\u2013245 represent immunodominant epitopes recognized by pSS sera in a significantly higher degree than by PBC sera, which react predominantly with AA 228\u2013 245 (<\/jats:bold>\n                                 <jats:bold>\n                                    P\n                                 <\/jats:bold>\n                                 <jats:bold>&lt; .0001). Anti\u2013La(SS\u2013B) antibodies were significantly associated with anti\u201352\u2013kd Ro(SS\u2013A) in sera from patients with pSS compared with PBC patients (<\/jats:bold>\n                                 <jats:bold>\n                                    P\n                                 <\/jats:bold>\n                                 <jats:bold>&lt; .025). Thus, the antibody response to 52\u2013kd Ro(SS\u2013A) in PBC appears to be induced differently than in pSS. Although a limited immune response to 52\u2013kd Ro(SS\u2013A) occurs in PBC\/SS patients, a more extended epitope spreading is evident in patients with pSS.<\/jats:bold>\n                              <\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.510240616","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T00:00:39Z","timestamp":1100217639000},"page":"1404-1407","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":16,"title":["Differential Recognition of the 52\u2013Kd Ro(Ss\u2013A) Antigen by Sera From Patients With Primary Biliary Cirrhosis and Primary Sjogren's Syndrome"],"prefix":"10.1097","volume":"24","author":[{"given":"T","family":"Dorner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Feist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Held","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Conrad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G R","family":"Burmester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Hiepe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.510240616","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199612000-00016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:17:46Z","timestamp":1733051866000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199612000-00016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.510240616","relation":{},"ISSN":["0270-9139"],"issn-type":[{"type":"print","value":"0270-9139"}],"subject":[],"published":{"date-parts":[[1996,12]]}}}