{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T10:37:54Z","timestamp":1772793474240,"version":"3.50.1"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":6848,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>\n                                 <jats:bold>Peripheral blood mononuclear cells (PBMC) of patients with autoimmune hepatitis (AIH) and controls were studied for their proliferative response to six overlapping synthetic peptides covering the 33\u2013amino acid immunodominant region of cytochrome P450IID6, the main target antigen of LKM\u20131 antibody\u2013positive type II AIH. PBMC from 8 of 8 type II AIH patients (100%), 6 of 12 LKM\u20131 antibody\u2013negative type I AIH patients (50%), but only 4 of 31 patients with chronic hepatitis C (12.9%) reacted with a 23\u2013amino acid LKM peptide and mainly with a shorter 18\u2013amino acid LKM peptide. Follow\u2013up showed that LKM\u2013specific T\u2013cell responses decreased after immunosuppression had started. Fine specificity, HLA restriction, and cytokine release of LKM\u2013specific T cells were analyzed with 16 CD4+ peptide\u2013specific T\u2013cell lines and 21 CD4+ T\u2013cell clones isolated and expanded from blood and liver tissue of six AIH patients. Activated LKM\u2013specific T cells released interferon gamma (IFN\u2013\u03b3) but no or little interleukin\u20134. In three AIH patients, PBMC showed specific recognition of autologous LKM\u2013specific T cells, suggesting the presence of a regulatory T\u2013cell network. These T cells also showed the CD4+ phenotype and secreted large amounts of IFN\u2013\u03b3. Furthermore, it was assessed that the regulatory T\u2013cell response is clonotypic. To conclude, we describe a major T\u2013cell epitope in AIH that was recognized by Th1 helper cells isolated from blood and liver tissue. This autoreactive T\u2013cell response correlated widely with disease activity and LKM\u20131 antibody status and seemed to be regulated by anticlonotypic T cells.<\/jats:bold>\n                              <\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.510240619","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T00:00:39Z","timestamp":1100217639000},"page":"1416-1421","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":66,"title":["Autoreactive Cd4+ Lkm\u2013Specific and Anticlonotypic T\u2013Cell Responses in Lkm\u20131 Antibody\u2013Positive Autoimmune Hepatitis"],"prefix":"10.1097","volume":"24","author":[{"given":"H F","family":"Lohr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J F","family":"Schlaak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A W","family":"Lohse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W O","family":"Bocher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Arenz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Gerken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K M","family":"Buschenfelde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.510240619","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199612000-00019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:18:03Z","timestamp":1733051883000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199612000-00019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.510240619","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1996,12]]}}}