{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:23:48Z","timestamp":1791890628809,"version":"4.3.4"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":6483,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Tumor necrosis factor (TNF)\u03b1, a pivotal cytokine involved in inflammation, is produced primarily by Kupffer cells in the liver. It has been shown that inactivation of Kupffer cells prevents alcohol-induced liver injury; therefore, the purpose of this study was to determine if neutralizing anti-TNF-\u03b1 antibody is also effective. Male Wistar rats were exposed to ethanol (11 to 12 g \u00b7 kg<jats:sup>-1<\/jats:sup> \u00b7 d<jats:sup>-1<\/jats:sup>) continuously for up to 4 weeks via intragastric feeding using an enteral feeding model. Before ethanol exposure, polyclonal anti-mouse TNF-\u03b1 rabbit serum was injected (2.0 mg\/kg intravenously). There were no significant differences in body weight, mean ethanol concentration, or cyclic patterns of ethanol in urine when ethanol-and ethanol plus antibody-treated groups were compared. Expression of TNF- \u03b1 and macrophage inflammatory protein 2 (MIP-2) messenger RNA (mRNA), determined using reverse transcription-polymerase chain reaction, was three- to four-fold higher in livers of ethanol-treated rats than in those of rats fed an ethanol-free, high-fat control diet. In addition, MIP-2 levels were also elevated when detected by Northern blot analysis. Anti-TNF-\u03b1 antibody did not affect expression of mRNA for interleukin (IL) 1\u03b1, IL-6, transforming growth factor \u03b21, or TNF-\u03b1. However, MIP-2 mRNA expression, which is regulated by TNF-\u03b1, was decreased significantly by anti-TNF-\u03b1 antibody treatment. Serum aspartate transaminase levels were elevated in ethanol-treated rats to 136 \u00b1 12 IU\/L after 4 weeks but only reached 90 \u00b1 5 IU\/L (<jats:italic toggle=\"yes\">P<\/jats:italic> &lt; .05) in rats treated with anti-TNF-\u03b1 antibody. The hepatic inflammation and necrosis observed in ethanol-fed rats were attenuated significantly by antibody treatment, and steatosis was not. These results support the hypothesis that TNF-\u03b1 plays an important role in inflammation and necrosis in alcohol-induced liver injury and that treatment with anti-TNF-\u03b1 antibody may be therapeutically useful in this disease.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.510260621","type":"journal-article","created":{"date-parts":[[2004,7,27]],"date-time":"2004-07-27T23:26:58Z","timestamp":1090970818000},"page":"1530-1537","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":431,"title":["Antibodies to tumor necrosis factor alfa attenuate hepatic necrosis and inflammation caused by chronic exposure to ethanol in the rat"],"prefix":"10.1097","volume":"26","author":[{"given":"Y","family":"Iimuro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Gallucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M I","family":"Luster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Kono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R G","family":"Thurman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.510260621","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199712000-00022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T15:54:10Z","timestamp":1738943650000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199712000-00022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12]]},"references-count":0,"aliases":["10.1053\/jhep.1997.v26.pm0009397994"],"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.510260621","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[1997,12]]}}}