{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:45:42Z","timestamp":1761518742983,"version":"3.30.0"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":5906,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,7]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>The characteristic serological feature of primary biliary cirrhosis (PBC) is the presence of antimitochondrial antibodies (AMAs), and the major proteins recognized by AMAs are subunits of the 2-oxo acid dehydrogenase complexes (2-OADC), including the E2 components of the pyruvate dehydrogenase complex (PDC), the 2-oxo-glutarate dehydrogenase complex (OGDC), the branched-chain 2-oxoacid dehydrogenase complex (BCOADC), the E3 binding protein (E3BP or protein X) and the E1a component of mammalian PDC. Previous work has postulated that either E3BP, or a molecule cross-reactive with the PDC-E2 molecule, is uniquely expressed on the surface of biliary epithelial cells in PBC. To address this issue, we performed <jats:italic toggle=\"yes\">in situ<\/jats:italic> hybridization for all of the major 2-OADC components at the mRNA level, including PDC-E2, BCOADC-E2, OGDC-E2, PDC-E1a, BCOADC-E1a, OGDC-E1, and E3BP using 13 PBC and 9 control livers using 7 mitochondrial antisense probes. In both PBC and controls, the expression of all 2-OADC component mRNA studied herein were found in hepatocytes and infiltrating mononuclear cells, without significant differences. Interestingly, however, despite published data on immunohistochemical staining, interlobular bile ducts including the injured bile ducts in PBC were generally negative or only faintly positive, with the exception of 1 bile duct in 1 of 13 cases of PBC and 1 of 9 control liver specimens. Moreover, confocal microscopic examination and image analysis revealed that the mRNA signal intensity of each of the 2-OADC components in the bile ducts of PBC was relatively lower in comparison with control liver diseases. These data suggest that continuous enhanced synthesis of the 2-OADC components is not likely to be occurring in the biliary epithelial cells in PBC, and that an increase of PDC-E2 or E3BP immunoreactivity in PBC is caused by exogenous imported or cross-reactive molecules<\/jats:p>\n          <\/jats:sec>","DOI":"10.1002\/hep.510300145","type":"journal-article","created":{"date-parts":[[2004,7,28]],"date-time":"2004-07-28T07:26:03Z","timestamp":1090999563000},"page":"36-45","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":17,"title":["In situ nucleic acid detection of PDC-E2, BCOADC-E2, OGDC-E2, PDC-E1\u03b1, BCOADC-E1\u03b1, OGDC-E1,and the E3 binding protein (protein X) in primary biliary cirrhosis"],"prefix":"10.1097","volume":"30","author":[{"given":"Kenichi","family":"Harada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiko","family":"Sudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Kono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Ozaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Tsuneyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric M.","family":"Gershwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuni","family":"Nakanuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"BIB1","volume-title":"Liver biopsy interpretation","author":"Scheuer","year":"1980","unstructured":"Liver biopsy interpretation. 3rd ed. London: Bailliere Tindall, 1980."},{"key":"BIB2","first-page":"387","volume":"46","author":"Rubin","year":"1965","journal-title":"Am J Pathol"},{"key":"BIB3","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1056\/NEJM199611213352107","volume":"335","author":"Kaplan","year":"1996","journal-title":"N Engl J Med"},{"key":"BIB4","doi-asserted-by":"crossref","first-page":"8654","DOI":"10.1073\/pnas.85.22.8654","volume":"85","author":"Fussey","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BIB5","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.4049\/jimmunol.141.7.2321","volume":"141","author":"Van de Water","year":"1998","journal-title":"J Immunol"},{"key":"BIB6","doi-asserted-by":"crossref","first-page":"7317","DOI":"10.1073\/pnas.85.19.7317","volume":"85","author":"Coppel","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BIB7","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1002\/hep.1840040428","volume":"4","author":"Kaplan","year":"1984","journal-title":"Hepatology"},{"key":"BIB8","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/0016-5085(91)80033-6","volume":"100","author":"Gershwin","year":"1991","journal-title":"Gastroenterology"},{"key":"BIB9","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/hep.1840080128","volume":"8","author":"Gershwin","year":"1998","journal-title":"Hepatology"},{"key":"BIB10","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/S0140-6736(88)91894-6","volume":"1","author":"Yeaman","year":"1988","journal-title":"Lancet"},{"key":"BIB11","doi-asserted-by":"crossref","first-page":"3815","DOI":"10.4049\/jimmunol.142.11.3815","volume":"142","author":"Fregeau","year":"1989","journal-title":"J Immunol"},{"key":"BIB12","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1002\/hep.1840110611","volume":"11","author":"Fregeau","year":"1990","journal-title":"Hepatology"},{"key":"BIB13","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/hep.1840100202","volume":"10","author":"Surh","year":"1989","journal-title":"Hepatology"},{"key":"BIB14","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1042\/cs0770365","volume":"77","author":"Fussey","year":"1989","journal-title":"Clin Sci"},{"key":"BIB15","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.4049\/jimmunol.144.5.1671","volume":"144","author":"Fregeau","year":"1990","journal-title":"J Immunol"},{"key":"BIB16","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1016\/0016-5085(79)90396-2","volume":"76","author":"Nakanuma","year":"1979","journal-title":"Gastroenterology"},{"key":"BIB17","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/0046-8177(95)90120-5","volume":"26","author":"Nakanuma","year":"1995","journal-title":"Hum Pathol"},{"key":"BIB18","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1002\/hep.1840140307","volume":"14","author":"Joplin","year":"1991","journal-title":"Hepatology"},{"key":"BIB19","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1002\/hep.1840190610","volume":"19","author":"Joplin","year":"1994","journal-title":"Hepatology"},{"key":"BIB20","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0140-6736(92)91001-O","volume":"339","author":"Joplin","year":"1992","journal-title":"Lancet"},{"key":"BIB21","first-page":"1031","volume":"21","author":"Tsuneyama","year":"1995","journal-title":"Hepatology"},{"key":"BIB22","doi-asserted-by":"crossref","first-page":"2653","DOI":"10.1172\/JCI116504","volume":"91","author":"Van de Water","year":"1993","journal-title":"J Clin Invest"},{"key":"BIB23","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1002\/hep.510240517","volume":"24","author":"Van de Water","year":"1996","journal-title":"Hepatology"},{"key":"BIB24","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1053\/gast.1997.v113.pm9352878","volume":"113","author":"Joplin","year":"1997","journal-title":"Gastroenterology"},{"key":"BIB25","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/hep.510250106","volume":"25","author":"Harada","year":"1997","journal-title":"Hepatology"},{"key":"BIB26","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/BF00432479","volume":"379","author":"Ludwig","year":"1978","journal-title":"Virchows Arch A Pathol Anat Histol"},{"key":"BIB27","first-page":"69","volume":"412","author":"van Eyken","year":"1987","journal-title":"Virchows Arch A Pathol Anat Histopathol"},{"key":"BIB28","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1097\/00000478-199708000-00006","volume":"21","author":"Tickoo","year":"1997","journal-title":"Am J Surg Pathol"},{"key":"BIB29","doi-asserted-by":"crossref","first-page":"3367","DOI":"10.4049\/jimmunol.144.9.3367","volume":"144","author":"Surh","year":"1990","journal-title":"J Immunol"},{"key":"BIB30","doi-asserted-by":"crossref","first-page":"7398","DOI":"10.1016\/S0021-9258(18)48250-6","volume":"262","author":"Dahl","year":"1987","journal-title":"J Biol Chem"},{"key":"BIB31","doi-asserted-by":"crossref","first-page":"19746","DOI":"10.1074\/jbc.272.32.19746","volume":"272","author":"Harris","year":"1997","journal-title":"J Biol Chem"},{"key":"BIB32","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1073\/pnas.89.5.1963","volume":"89","author":"Koike","year":"1992","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BIB33","first-page":"360","volume":"1216","author":"Nakano","year":"1993","unstructured":"et al. Human dihydrolipoamide succinyltransferase: cDNA cloning and localization on chromosome 14q24.2-q24.3. 1993; 1216:360-368. MEDLINE"},{"key":"BIB34","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0378-1119(88)90390-3","volume":"69","author":"Zhang","year":"1988","journal-title":"Gene"},{"key":"BIB35","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1021\/bi00406a025","volume":"27","author":"Lau","year":"1988","journal-title":"Biochemistry"},{"key":"BIB36","doi-asserted-by":"crossref","first-page":"89","DOI":"10.4049\/jimmunol.146.1.89","volume":"146","author":"Van de Water","year":"1991","journal-title":"J Immunol"},{"key":"BIB37","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1084\/jem.181.5.1835","volume":"181","author":"Shimoda","year":"1995","journal-title":"J Exp Med"},{"key":"BIB38","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/S0168-8278(05)80276-4","volume":"18","author":"Lohr","year":"1993","journal-title":"J Hepatol"},{"key":"BIB39","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1002\/path.1711750310","volume":"175","author":"Yasoshima","year":"1995","journal-title":"J Pathol"},{"key":"BIB40","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/0046-8177(91)90127-B","volume":"22","author":"Nakanuma","year":"1991","journal-title":"Hum Pathol"},{"key":"BIB41","doi-asserted-by":"crossref","first-page":"2647","DOI":"10.4049\/jimmunol.144.7.2647","volume":"144","author":"Surh","year":"1990","journal-title":"J Immunol"},{"key":"BIB42","first-page":"505","volume":"22","author":"Leung","year":"1995","journal-title":"Hepatology"},{"key":"BIB43","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1002\/hep.510230307","volume":"23","author":"Moteki","year":"1996","journal-title":"Hepatology"},{"key":"BIB44","doi-asserted-by":"crossref","first-page":"3224","DOI":"10.1096\/fasebj.4.14.2227213","volume":"4","author":"Patel","year":"1990","journal-title":"FASEB J"},{"key":"BIB45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1989.tb14983.x","volume":"573","author":"Perham","year":"1989","journal-title":"Ann N Y Acad Sci"},{"key":"BIB46","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1002\/hep.1840130314","volume":"13","author":"Fussey","year":"1991","journal-title":"Hepatology"},{"key":"BIB47","doi-asserted-by":"crossref","first-page":"3987","DOI":"10.1073\/pnas.87.10.3987","volume":"87","author":"Fussey","year":"1990","journal-title":"Proc Natl Acad Sci U S A"},{"key":"BIB48","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1002\/hep.1840100321","volume":"10","author":"Flannery","year":"1989","journal-title":"Hepatology"},{"key":"BIB49","doi-asserted-by":"crossref","first-page":"3525","DOI":"10.4049\/jimmunol.138.10.3525","volume":"138","author":"Gershwin","year":"1987","journal-title":"J Immunol"},{"key":"BIB50","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1136\/gut.36.6.931","volume":"36","author":"Butler","year":"1995","journal-title":"Gut"},{"key":"BIB51","first-page":"473","volume":"35","author":"Butler","year":"1995","journal-title":"Evidence for a molecular mimicry mechanism in the pathogenesis of primary biliary cirrhosis? Biochem Mol Biol Int"},{"key":"BIB52","first-page":"345","volume":"32","author":"Tobe","year":"1982","journal-title":"Acta Pathol Jpn"},{"key":"BIB53","first-page":"824","volume":"12","author":"Naka","year":"1979","journal-title":"J Clin Electron Microscopy"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhep.510300145","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-199907000-00006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:25:29Z","timestamp":1733052329000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-199907000-00006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,7]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1999]]}},"URL":"https:\/\/doi.org\/10.1002\/hep.510300145","relation":{},"ISSN":["0270-9139"],"issn-type":[{"type":"print","value":"0270-9139"}],"subject":[],"published":{"date-parts":[[1999,7]]}}}