{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:06:05Z","timestamp":1790809565832,"version":"4.1.0"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Communications"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>Hepatoblastoma (HBL) is the most common pediatric liver cancer. In this malignant neoplasm, beta\u2010catenin protein accumulates and increases Wnt signaling due to recurrent activating mutations in the catenin\u2010beta 1 (<jats:italic toggle=\"yes\">CTNNB1<\/jats:italic>) gene. Therefore, beta\u2010catenin is a key therapeutic target in HBL. However, controlling beta\u2010catenin production with therapeutic molecules has been challenging. New biological studies could provide alternative therapeutic solutions for the treatment of HBL, especially for advanced tumors and metastatic disease. In this study, we identified microRNAs (miRNAs) that target beta\u2010catenin and block HBL cell proliferation <jats:italic toggle=\"yes\">in vitro<\/jats:italic> and tumor growth <jats:italic toggle=\"yes\">in vivo<\/jats:italic>. Using our dual\u2010fluorescence\u2010FunREG system, we screened a library of 1,712 miRNA mimics and selected candidates inhibiting <jats:italic toggle=\"yes\">CTNNB1<\/jats:italic> expression through interaction with its untranslated regions. After validating the regulatory effect of nine miRNAs on beta\u2010catenin in HBL cells, we measured their expression in patient samples. Let\u20107i\u20103p, miR\u2010449b\u20103p, miR\u2010624\u20105p, and miR\u2010885\u20105p were decreased in tumors compared to normal livers. Moreover, they inhibited HBL cell growth and Wnt signaling activity <jats:italic toggle=\"yes\">in vitro<\/jats:italic> partly through beta\u2010catenin down\u2010regulation. Additionally, miR\u2010624\u20105p induced cell senescence <jats:italic toggle=\"yes\">in vitro<\/jats:italic>, blocked experimental HBL growth <jats:italic toggle=\"yes\">in vivo<\/jats:italic>, and directly targeted the beta\u2010catenin 3\u2032\u2010untranslated region. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: Our results shed light on how beta\u2010catenin\u2010regulating miRNAs control HBL progression through Wnt signaling inactivation. In particular, miR\u2010624\u20105p may constitute a promising candidate for miRNA replacement therapy for HBL patients. (<jats:italic toggle=\"yes\">Hepatology Communications<\/jats:italic> 2017;1:168\u2010183)<\/jats:p>","DOI":"10.1002\/hep4.1029","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T17:09:27Z","timestamp":1491498567000},"page":"168-183","source":"Crossref","is-referenced-by-count":54,"title":["MicroRNA therapy inhibits hepatoblastoma growth in vivo by targeting \u03b2\u2010catenin and Wnt signaling"],"prefix":"10.1097","volume":"1","author":[{"given":"Emilie","family":"Indersie","sequence":"first","affiliation":[{"name":"Univ. Bordeaux, Inserm, GREF, U1053, 33076,Bordeaux,France"},{"name":"Univ. Bordeaux, Inserm, BMGIC, U1035, 33076,Bordeaux,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Lesjean","sequence":"additional","affiliation":[{"name":"Univ. Bordeaux, Inserm, GREF, U1053, 33076,Bordeaux,France"},{"name":"Univ. 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