{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:06:16Z","timestamp":1792681576299,"version":"4.5.0"},"reference-count":22,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2018,12,28]],"date-time":"2018-12-28T00:00:00Z","timestamp":1545955200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatol Commun"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>Liver sinusoidal endothelial cells (LSECs) possess fenestrae, which are key for the exchange between blood and hepatocytes. Alterations in their number or diameter have important implications for hepatic function in liver diseases. They are lost early in the development of hepatic fibrosis through a process called capillarization. In this study, we aimed to demonstrate whether <jats:italic toggle=\"yes\">in vitro<\/jats:italic> dedifferentiated LSECs that have lost fenestrae are able to re\u2010form these structures. Using stimulated emission depletion super\u2010resolution microscopy in combination with transmission electron microscopy, we analyzed fenestrae formation in a model mimicking the capillarization process <jats:italic toggle=\"yes\">in vitro<\/jats:italic>. Actin is known to be involved in fenestrae regulation in differentiated LSECs. Using cytochalasin D, an actin\u2010depolymerizing agent, we demonstrated that dedifferentiated LSECs remain capable of forming fenestrae. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: We provide a new insight into the complex role of actin in fenestrae formation and in the control of their size and show that LSEC fenestrae re\u2010formation is possible, suggesting that this process could be used during fibrosis regression to try to restore exchanges and hepatocyte functions.<\/jats:p>","DOI":"10.1002\/hep4.1301","type":"journal-article","created":{"date-parts":[[2018,12,28]],"date-time":"2018-12-28T16:04:34Z","timestamp":1546013074000},"page":"213-219","source":"Crossref","is-referenced-by-count":30,"title":["Actin Depolymerization in Dedifferentiated Liver Sinusoidal Endothelial Cells Promotes Fenestrae Re\u2010Formation"],"prefix":"10.1097","volume":"3","author":[{"given":"Julie","family":"Di Martino","sequence":"first","affiliation":[{"name":"INSERM, UMR1053,Bariton\u2010Bordeaux Research in Translational Oncology,Bordeaux,France"},{"name":"Universit\u00e9 de Bordeaux,Bordeaux,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Mascalchi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bordeaux,Bordeaux,France"},{"name":"Bordeaux Imaging Center,Bordeaux,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Legros","sequence":"additional","affiliation":[{"name":"Plateforme Aquitaine de Caract\u00e9risation des Mat\u00e9riaux,Pessac,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Lacomme","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bordeaux,Bordeaux,France"},{"name":"Bordeaux Imaging Center,Bordeaux,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Gontier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de 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