{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:26:59Z","timestamp":1787563619302,"version":"build-2736575974"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2019,11,23]],"date-time":"2019-11-23T00:00:00Z","timestamp":1574467200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatol Commun"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    Hyperoxidized albumin promotes inflammation and modulates several immune cells in severe alcoholic hepatitis (SAH). Platelets mediate inflammation by interacting with immune cells, endothelium, and other cells. The role of hyperoxidized albumin in platelet activation and alteration of platelet phenotype\/functions is not known. Quantitative platelet proteomics performed in 10 patients with SAH was compared with 10 patients with alcoholic cirrhosis and 10 healthy controls, respectively. Dysregulated pathways were identified and validated in a separate cohort (n\u00a0=\u00a040). Healthy platelets were exposed to patient plasma or purified albumin or\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    modified albumin (human\u2010mercaptalbumin, humannonmercaptalbumin\u20101, and human nonmercaptalbumin\n                    <jats:italic toggle=\"yes\">2<\/jats:italic>\n                    ) in the presence or absence of CD36 blockade, and platelet secretome was analyzed. Two hundred and two up\u2010regulated proteins linked to platelet activation, complement regulation, lipid transportation, and 321 down\u2010regulated proteins related to platelet hemostasis and coagulation (fold change\u00a0\u00b1\u00a01.5,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u00a0&lt;\u00a00.01) were identified. Blood transcription module enrichment showed an inflammatory phenotype of SAH platelet. Increased level of platelet factor\u20104, P\u2010selectin, and soluble cluster of differentiation\u201040 ligand correlated with severity (Model for End\u2010Stage Liver Disease score, r\u00a0&gt;\u00a00.3,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05) in SAH. Transcripts linked to platelet activation (increased) and granular secretions (decreased in SAH) correlated with disease severity. SNARE (soluble\u2010N\u2010ethylmaleimide\u2010sensitive\u2010factor\u2010activating\u2010protein\u2010receptor) complex proteins (SNAP\u201023 [synaptosomal\u2010associated protein 23] and VAMP\u20108 [vesicle\u2010associated membrane protein 3]) were down\u2010regulated in SAH platelets (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05).\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    stimulation of healthy platelets showed enhanced activation with patient plasma, or purified albumin\u2010treatment blocking of CD36 blunted this effect (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05).\n                    <jats:italic toggle=\"yes\">Ex vivo<\/jats:italic>\n                    modified albumin (primarily nonmercaptalbumin\u2013human nonmercaptalbumin 2 [HNA2; 1\u00a0mg\/mL]) showed high activation and aggregation and intracellular reactive oxygen species production in healthy platelets (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05), which significantly reduced after CD36 neutralization. Platelet secretome showed reduced inflammatory mediators and increased repair proteins.\n                    <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic>\n                    Hyperoxidized albumin triggers platelet activation (possibly through the CD36 receptor), promotes inflammation and oxidative stress, and contributes to disease severity in patients with SAH.\n                  <\/jats:p>","DOI":"10.1002\/hep4.1440","type":"journal-article","created":{"date-parts":[[2019,11,23]],"date-time":"2019-11-23T03:40:51Z","timestamp":1574480451000},"page":"50-65","source":"Crossref","is-referenced-by-count":22,"title":["Hyperoxidized Albumin Modulates Platelets and Promotes Inflammation Through CD36 Receptor in Severe Alcoholic Hepatitis"],"prefix":"10.1097","volume":"4","author":[{"given":"Adil","family":"Bhat","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukanta","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudrishti","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashish","family":"Vyas","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojahidul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishak C.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenu","family":"Bajpai","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakhi","family":"Maiwall","sequence":"additional","affiliation":[{"name":"Department of Hepatology,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3938-362X","authenticated-orcid":false,"given":"Jaswinder Singh","family":"Maras","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0544-5610","authenticated-orcid":false,"given":"Shiv K.","family":"Sarin","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Medicine,Institute of Liver and Biliary Sciences,New Delhi,India"},{"name":"Department of Hepatology,Institute of Liver and Biliary Sciences,New Delhi,India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2019,11,23]]},"reference":[{"key":"hep41440-bib-0001-20240114","article-title":"Survival from alcoholic hepatitis has not improved over 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