{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:45:04Z","timestamp":1790959504411,"version":"4.1.0"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T00:00:00Z","timestamp":1580947200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatol Commun"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>Alcoholic liver disease (ALD) is a leading cause of cirrhosis in the United States, which is characterized by extensive deposition of extracellular matrix proteins and formation of a fibrous scar. Hepatic stellate cells (HSCs) are the major source of collagen type 1 producing myofibroblasts in ALD fibrosis. However, the mechanism of alcohol\u2010induced activation of human and mouse HSCs is not fully understood. We compared the gene\u2010expression profiles of primary cultured human HSCs (hHSCs) isolated from patients with ALD (n\u00a0=\u00a03) or without underlying liver disease (n\u00a0=\u00a04) using RNA\u2010sequencing analysis. Furthermore, the gene\u2010expression profile of ALD hHSCs was compared with that of alcohol\u2010activated mHSCs (isolated from intragastric alcohol\u2010fed mice) or CCl<jats:sub>4<\/jats:sub>\u2010activated mouse HSCs (mHSCs). Comparative transcriptome analysis revealed that ALD hHSCs, in addition to alcohol\u2010activated and CCl<jats:sub>4<\/jats:sub>\u2010activated mHSCs, share the expression of common HSC activation (<jats:italic toggle=\"yes\">Col1a1<\/jats:italic> [collagen type I alpha 1 chain], <jats:italic toggle=\"yes\">Acta1<\/jats:italic> [actin alpha 1, skeletal muscle], <jats:italic toggle=\"yes\">PAI1<\/jats:italic> [plasminogen activator inhibitor\u20101], <jats:italic toggle=\"yes\">TIMP1<\/jats:italic> [tissue inhibitor of metalloproteinase 1], and <jats:italic toggle=\"yes\">LOXL2<\/jats:italic> [lysyl oxidase homolog 2]), indicating that a common mechanism underlies the activation of human and mouse HSCs. Furthermore, alcohol\u2010activated mHSCs most closely recapitulate the gene\u2010expression profile of ALD hHSCs. We identified the genes that are similarly and uniquely up\u2010regulated in primary cultured alcohol\u2010activated hHSCs and freshly isolated mHSCs, which include <jats:italic toggle=\"yes\">CSF1R<\/jats:italic> (macrophage colony\u2010stimulating factor 1 receptor), <jats:italic toggle=\"yes\">PLEK<\/jats:italic> (pleckstrin), <jats:italic toggle=\"yes\">LAPTM5<\/jats:italic> (lysosmal\u2010associated transmembrane protein 5), <jats:italic toggle=\"yes\">CD74<\/jats:italic> (class I transactivator, the invariant chain), <jats:italic toggle=\"yes\">CD53<\/jats:italic>, <jats:italic toggle=\"yes\">MMP9<\/jats:italic> (matrix metallopeptidase 9), <jats:italic toggle=\"yes\">CD14<\/jats:italic>, <jats:italic toggle=\"yes\">CTSS<\/jats:italic> (cathepsin S), <jats:italic toggle=\"yes\">TYROBP<\/jats:italic> (TYRO protein tyrosine kinase\u2010binding protein), and <jats:italic toggle=\"yes\">ITGB2<\/jats:italic> (integrin beta\u20102), and other genes (compared with CCl<jats:sub>4<\/jats:sub>\u2010activated mHSCs). <jats:italic toggle=\"yes\">Conclusion:<\/jats:italic> We identified genes in alcohol\u2010activated mHSCs from intragastric alcohol\u2010fed mice that are largely consistent with the gene\u2010expression profile of primary cultured hHSCs from patients with ALD. These genes are unique to alcohol\u2010induced HSC activation in two species, and therefore may become targets or readout for antifibrotic therapy in experimental models of ALD.<\/jats:p>","DOI":"10.1002\/hep4.1483","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T07:15:51Z","timestamp":1581059751000},"page":"606-626","source":"Crossref","is-referenced-by-count":32,"title":["Primary Alcohol\u2010Activated Human and Mouse Hepatic Stellate Cells Share Similarities in Gene\u2010Expression Profiles"],"prefix":"10.1097","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7608-5819","authenticated-orcid":false,"given":"Xiao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Surgery,University of California, San Diego,La Jolla,CA"},{"name":"Department of Medicine,University of California, San Diego,La Jolla,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara 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Jolla,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Diggle","sequence":"additional","affiliation":[{"name":"Department of Medicine,University of California, San Diego,La Jolla,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Medicine,University of California, San Diego,La Jolla,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Wu","sequence":"additional","affiliation":[{"name":"Southern California Research Center for ALPD & Cirrhosis,Keck School of Medicine of the University of Southern California,Los Angeles,CA"},{"name":"Department of Pathology,Keck School of Medicine of the University of Southern California,Los Angeles,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie Q.","family":"Pan","sequence":"additional","affiliation":[{"name":"Southern California Research Center for ALPD & Cirrhosis,Keck School of Medicine of the University of Southern California,Los Angeles,CA"},{"name":"Department of Pathology,Keck School of Medicine of the University of Southern California,Los Angeles,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibu","family":"Chen","sequence":"additional","affiliation":[{"name":"Bioinformatics Services,Keck School of Medicine of the University of Southern California,Los Angeles,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Dorko","sequence":"additional","affiliation":[{"name":"Samsara Sciences,San Diego,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Presnell","sequence":"additional","affiliation":[{"name":"Samsara Sciences,San Diego,CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Benner","sequence":"additional","affiliation":[{"name":"Department of Medicine,University of California, San Diego,La 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