{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T13:40:31Z","timestamp":1731678031700,"version":"3.28.0"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","license":[{"start":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T00:00:00Z","timestamp":1648684800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["19H03638","20K08281"],"award-info":[{"award-number":["19H03638","20K08281"]}]},{"award":["21fk0210047h0003","21fk0210058s0703","21fk0210072s0602","21fk0210065s0602","21fk0210067s0902"],"award-info":[{"award-number":["21fk0210047h0003","21fk0210058s0703","21fk0210072s0602","21fk0210065s0602","21fk0210067s0902"]}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatol Commun"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>The method of analyzing individual resistant hepatitis C virus (HCV) by a combination of haplotyping and resistance\u2010associated substitution (RAS) has not been fully elucidated because conventional sequencing has only yielded short and fragmented viral genomes. We performed haplotype analysis of HCV mutations in 12 asunaprevir\/daclatasvir treatment\u2010failure cases using the Oxford Nanopore sequencer. This enabled single\u2010molecule long\u2010read sequencing using rolling circle amplification (RCA) for correction of the sequencing error. RCA of the circularized reverse\u2010transcription polymerase chain reaction products successfully produced DNA longer than 30 kilobase pairs (kb) containing multiple tandem repeats of a target 3 kb HCV genome. The long\u2010read sequencing of these RCA products could determine the original sequence of the target single molecule as the consensus nucleotide sequence of the tandem repeats and revealed the presence of multiple viral haplotypes with the combination of various mutations in each host. In addition to already known signature RASs, such as NS3\u2010D168 and NS5A\u2010L31\/Y93, there were various RASs specific to a different haplotype after treatment failure. The distribution of viral haplotype changed over time; some haplotypes disappeared without acquiring resistant mutations, and other haplotypes, which were not observed before treatment, appeared after treatment. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: The combination of various mutations other than the known signature RAS was suggested to influence the kinetics of individual HCV quasispecies in the direct\u2010acting antiviral treatment. HCV haplotype dynamic analysis will provide novel information on the role of HCV diversity within the host, which will be useful for elucidating the pathological mechanism of HCV\u2010related diseases.<\/jats:p>","DOI":"10.1002\/hep4.1929","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T12:08:02Z","timestamp":1648728482000},"page":"1634-1651","update-policy":"http:\/\/dx.doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of direct\u2010acting antiviral\u2010resistant hepatitis C virus haplotype diversity by single\u2010molecule and long\u2010read sequencing"],"prefix":"10.1097","volume":"6","author":[{"given":"Kozue","family":"Yamauchi","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuaki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leona","family":"Osawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuya","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Komiyama","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natsuko","family":"Nakakuki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitomi","family":"Takada","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Katoh","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Muraoka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichiro","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihisa","family":"Tatsumi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Takano","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumitake","family":"Amemiya","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuharu","family":"Fukasawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taisuke","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinya","family":"Maekawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Enomoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology,Faculty of Medicine,University of Yamanashi,Yamanashi,Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2022,3,31]]},"reference":[{"key":"hep41929-bib-0001-20241115","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1111\/hepr.13417","article-title":"Countermeasures against viral hepatitis B and C in Japan: an epidemiological point of view","volume":"49","year":"2019","journal-title":"Hepatol Res"},{"key":"hep41929-bib-0002-20241115","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1007\/s00535-018-1493-8","article-title":"The, \u201creal\u2010world\u201d efficacy and safety of DAAs for the treatment of HCV patients throughout Japan","volume":"53","year":"2018","journal-title":"J\u00a0Gastroenterol"},{"key":"hep41929-bib-0003-20241115","first-page":"686","article-title":"Hepatitis C guidance update: American Association for the Study of Liver Diseases\u2010Infectious Diseases Society of America Recommendations for Testing, Managing, and Treating Hepatitis C Virus Infection","volume":"2020","journal-title":"Hepatology"},{"key":"hep41929-bib-0004-20241115","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1136\/flgastro-2018-101114","article-title":"Overcoming barriers to hepatitis C elimination","volume":"10","year":"2019","journal-title":"Frontline Gastroenterol"},{"key":"hep41929-bib-0005-20241115","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/S0140-6736(18)32277-3","article-title":"Scaling up prevention and treatment towards the elimination of hepatitis C: a global mathematical model","volume":"393","year":"2019","journal-title":"Lancet"},{"key":"hep41929-bib-0006-20241115","doi-asserted-by":"crossref","first-page":"41660","DOI":"10.1038\/srep41660","article-title":"Baseline quasispecies selection and novel mutations contribute to emerging resistance\u2010associated substitutions in hepatitis C virus after direct\u2010acting antiviral treatment","volume":"7","year":"2017","journal-title":"Sci Rep"},{"key":"hep41929-bib-0007-20241115","doi-asserted-by":"crossref","first-page":"12541","DOI":"10.1128\/JVI.00826-13","article-title":"Deep\u2010sequencing analysis of the association between the quasispecies nature of the hepatitis C virus core region and disease progression","volume":"87","year":"2013","journal-title":"J Virol"},{"key":"hep41929-bib-0008-20241115","doi-asserted-by":"crossref","first-page":"27025","DOI":"10.1038\/srep27025","article-title":"Impact of HCV core gene quasispecies on hepatocellular carcinoma risk among HALT\u2010C trial patients","volume":"6","year":"2016","journal-title":"Sci Rep"},{"key":"hep41929-bib-0009-20241115","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/ijms19010023","article-title":"Hepatitis C virus: viral quasispecies and genotypes","volume":"19","year":"2017","journal-title":"Int J Mol Sci"},{"issue":"Suppl.1","key":"hep41929-bib-0010-20241115","first-page":"S2","article-title":"The history of hepatitis C virus (HCV): Basic research reveals unique features in phylogeny, evolution and the viral life cycle with new perspectives for epidemic control","volume":"65","year":"2016","journal-title":"J Hepatol"},{"key":"hep41929-bib-0011-20241115","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1053\/j.gastro.2017.11.007","article-title":"Patterns of resistance\u2010associated substitutions in patients with chronic HCV infection following treatment with direct\u2010acting antivirals","volume":"154","year":"2018","journal-title":"Gastroenterology"},{"key":"hep41929-bib-0012-20241115","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-4939-8976-8_1","article-title":"Overview of direct\u2010acting antiviral drugs and drug resistance of hepatitis C virus","volume":"1911","year":"2019","journal-title":"Methods Mol Biol"},{"key":"hep41929-bib-0013-20241115","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1073\/pnas.1805256116","article-title":"Data\u2010driven supervised learning of a viral protease specificity landscape from deep sequencing and molecular simulations","volume":"116","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"key":"hep41929-bib-0014-20241115","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/hep.27192","article-title":"Next\u2010generation sequencing sheds light on the natural history of hepatitis C infection in patients who fail treatment","volume":"61","year":"2015","journal-title":"Hepatology"},{"key":"hep41929-bib-0015-20241115","article-title":"Deep sequencing is an appropriate tool for the selection of unique hepatitis C virus (HCV) variants after single genomic amplification","volume":"12","year":"2017","journal-title":"PLoS One"},{"key":"hep41929-bib-0016-20241115","doi-asserted-by":"crossref","first-page":"4661","DOI":"10.3748\/wjg.v25.i32.4661","article-title":"Exploring the hepatitis C virus genome using single molecule real\u2010time sequencing","volume":"25","year":"2019","journal-title":"World J Gastroenterol"},{"key":"hep41929-bib-0017-20241115","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1111\/hepr.13339","article-title":"A case of genotype\u20103b hepatitis C virus in which the whole genome was successfully analyzed using third\u2010generation nanopore sequencing","volume":"49","year":"2019","journal-title":"Hepatol Res"},{"key":"hep41929-bib-0018-20241115","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s12864-021-07460-1","article-title":"Adaptation of Oxford nanopore technology for hepatitis C whole genome sequencing and identification of within\u2010host viral variants","volume":"22","year":"2021","journal-title":"BMC Genom"},{"key":"hep41929-bib-0019-20241115","doi-asserted-by":"crossref","first-page":"6783","DOI":"10.1021\/acs.analchem.9b00856","article-title":"High\u2010fidelity nanopore sequencing of ultra\u2010short DNA targets","volume":"91","year":"2019","journal-title":"Anal Chem"},{"key":"hep41929-bib-0020-20241115","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1186\/s12864-016-2575-8","article-title":"A method for near full\u2010length amplification and sequencing for six hepatitis C virus genotypes","volume":"17","year":"2016","journal-title":"BMC Genom"},{"key":"hep41929-bib-0021-20241115","doi-asserted-by":"crossref","first-page":"45605","DOI":"10.1038\/srep45605","article-title":"Evolution of multi\u2010drug resistant HCV clones from pre\u2010existing resistant\u2010associated variants during direct\u2010acting antiviral therapy determined by third\u2010generation sequencing","volume":"7","year":"2017","journal-title":"Sci Rep"},{"key":"hep41929-bib-0022-20241115","doi-asserted-by":"crossref","first-page":"6105","DOI":"10.1128\/JVI.03127-14","article-title":"Deep sequencing and phylogenetic analysis of variants resistant to interferon\u2010based protease inhibitor therapy in chronic hepatitis induced by genotype 1b hepatitis C virus","volume":"89","year":"2015","journal-title":"J Virol"},{"key":"hep41929-bib-0023-20241115","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1006\/viro.1998.9092","article-title":"Transcripts of a chimeric cDNA clone of hepatitis C virus genotype 1b are infectious in vivo","volume":"244","year":"1998","journal-title":"Virology"},{"key":"hep41929-bib-0024-20241115","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1016\/j.bbrc.2005.02.138","article-title":"Efficient replication of a full\u2010length hepatitis C virus genome, strain O, in cell culture, and development of a luciferase reporter system","volume":"329","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"hep41929-bib-0025-20241115","first-page":"E360","article-title":"Deep sequencing analysis of variants resistant to the non\u2010structural 5A inhibitor daclatasvir in patients with genotype 1b hepatitis C virus infection","volume":"44","year":"2014","journal-title":"Hepatol Res"},{"key":"hep41929-bib-0026-20241115","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1002\/hep.30647","article-title":"Evolutionary pathways to persistence of highly fit and resistant hepatitis C virus protease inhibitor escape variants","volume":"70","year":"2019","journal-title":"Hepatology"},{"key":"hep41929-bib-0027-20241115","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/S1665-2681(19)31164-0","article-title":"Inter and intrapatient evolution of hepatitis C virus","volume":"14","year":"2015","journal-title":"Ann Hepatol"},{"key":"hep41929-bib-0028-20241115","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1038\/s41598-020-59397-2","article-title":"Single\u2010molecular real\u2010time deep sequencing reveals the dynamics of multi\u2010drug resistant haplotypes and structural variations in the hepatitis C virus genome","volume":"10","year":"2020","journal-title":"Sci Rep"},{"key":"hep41929-bib-0029-20241115","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1002\/hep.25826","article-title":"Comprehensive analysis for viral elements and interleukin\u201028B polymorphisms in response to pegylated interferon plus ribavirin therapy in hepatitis C virus 1B infection","volume":"56","year":"2012","journal-title":"Hepatology"},{"key":"hep41929-bib-0030-20241115","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.meegid.2016.06.015","article-title":"Deep sequencing increases hepatitis C virus phylogenetic cluster detection compared to Sanger sequencing","volume":"43","year":"2016","journal-title":"Infect Genet Evol"},{"key":"hep41929-bib-0031-20241115","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1093\/bioinformatics\/bty202","article-title":"De novo haplotype reconstruction in viral quasispecies using paired\u2010end read guided path finding","volume":"34","year":"2018","journal-title":"Bioinformatics"},{"key":"hep41929-bib-0032-20241115","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3390\/v11040323","article-title":"Interpreting viral deep sequencing data with GLUE","volume":"11","year":"2019","journal-title":"Viruses"},{"key":"hep41929-bib-0033-20241115","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.gpb.2015.08.002","article-title":"PacBio sequencing and its applications","volume":"13","year":"2015","journal-title":"Genomics Proteomics Bioinformatics"},{"key":"hep41929-bib-0034-20241115","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.tim.2019.01.010","article-title":"Long\u2010read sequencing \u2013 a powerful tool in viral transcriptome research","volume":"27","year":"2019","journal-title":"Trends Microbiol"},{"key":"hep41929-bib-0035-20241115","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s13577-017-0168-8","article-title":"Advantages of genome sequencing by long\u2010read sequencer using SMRT technology in medical area","volume":"30","year":"2017","journal-title":"Hum Cell"},{"key":"hep41929-bib-0036-20241115","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s13742-016-0140-7","article-title":"INC\u2010Seq: accurate single molecule reads using nanopore sequencing","volume":"5","year":"2016","journal-title":"GigaScience"},{"key":"hep41929-bib-0037-20241115","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.antiviral.2015.12.005","article-title":"Analysis of hepatitis C NS5A resistance associated polymorphisms using ultra deep single molecule real time (SMRT) sequencing","volume":"126","year":"2016","journal-title":"Antiviral Res"},{"key":"hep41929-bib-0038-20241115","doi-asserted-by":"crossref","first-page":"7081","DOI":"10.1038\/s41598-019-43524-9","article-title":"Illumina and Nanopore methods for whole genome sequencing of hepatitis B virus (HBV)","volume":"9","year":"2019","journal-title":"Sci Rep"},{"key":"hep41929-bib-0039-20241115","doi-asserted-by":"crossref","first-page":"13296","DOI":"10.1038\/ncomms13296","article-title":"TruePrime is a novel method for whole\u2010genome amplification from single cells based on TthPrimPol","volume":"7","year":"2016","journal-title":"Nat Commun"},{"key":"hep41929-bib-0040-20241115","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1053\/j.gastro.2018.10.024","article-title":"Status of direct\u2010acting antiviral therapy for hepatitis C virus infection and remaining challenges","volume":"156","year":"2019","journal-title":"Gastroenterology"},{"key":"hep41929-bib-0041-20241115","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.jhep.2018.03.026","article-title":"EASL recommendations on treatment of hepatitis C 2018","volume":"69","year":"2018","journal-title":"J Hepatol"},{"key":"hep41929-bib-0042-20241115","article-title":"Hepatitis C virus deletion mutants are found in individuals chronically infected with genotype 1 hepatitis C virus in association with age, high viral load and liver inflammatory activity","volume":"10","year":"2015","journal-title":"PLoS One"},{"key":"hep41929-bib-0043-20241115","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1111\/j.1365-2893.2006.00760.x","article-title":"Identification of novel defective HCV clones in liver transplant recipients with recurrent HCV infection","volume":"13","year":"2006","journal-title":"J Viral Hepat"},{"key":"hep41929-bib-0044-20241115","doi-asserted-by":"crossref","first-page":"3645","DOI":"10.1128\/JCM.00676-13","article-title":"Dynamics of defective hepatitis C virus clones in reinfected liver grafts in liver transplant recipients: ultradeep sequencing analysis","volume":"51","year":"2013","journal-title":"J Clin Microbiol"},{"key":"hep41929-bib-0045-20241115","doi-asserted-by":"crossref","first-page":"2942","DOI":"10.3389\/fmicb.2018.02942","article-title":"HCV defective genomes promote persistent infection by modulating the viral life cycle","volume":"9","year":"2018","journal-title":"Front Microbiol"},{"key":"hep41929-bib-0046-20241115","article-title":"Genetic analysis of serum\u2010derived defective hepatitis C virus genomes revealed novel viral cis elements for virus replication and assembly","volume":"92","year":"2018","journal-title":"J Virol"},{"key":"hep41929-bib-0047-20241115","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.tim.2014.02.005","article-title":"Comparative analysis of the molecular mechanisms of recombination in hepatitis C virus","volume":"22","year":"2014","journal-title":"Trends Microbiol"},{"key":"hep41929-bib-0048-20241115","article-title":"Third\u2010generation sequencing in the clinical laboratory: exploring the advantages and challenges of nanopore sequencing","volume":"58","year":"2019","journal-title":"J Clin Microbiol"},{"key":"hep41929-bib-0049-20241115","doi-asserted-by":"crossref","first-page":"6272","DOI":"10.1038\/s41467-020-20075-6","article-title":"Analytical validity of nanopore sequencing for rapid SARS\u2010CoV\u20102 genome analysis","volume":"11","year":"2020","journal-title":"Nat Commun"},{"key":"hep41929-bib-0050-20241115","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1101\/gr.257188.119","article-title":"Complete characterization of the human immune cell transcriptome using accurate full\u2010length cDNA sequencing","volume":"30","year":"2020","journal-title":"Genome Res"}],"container-title":["Hepatology Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hep4.1929","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hep4.1929","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/10.1002\/hep4.1929","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T13:01:08Z","timestamp":1731675668000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/10.1002\/hep4.1929"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,31]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.1002\/hep4.1929","relation":{},"ISSN":["2471-254X","2471-254X"],"issn-type":[{"type":"print","value":"2471-254X"},{"type":"electronic","value":"2471-254X"}],"subject":[],"published":{"date-parts":[[2022,3,31]]},"assertion":[{"value":"2021-11-10","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-04","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}