{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T21:58:48Z","timestamp":1778018328465,"version":"3.51.4"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","license":[{"start":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T00:00:00Z","timestamp":1664236800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["Hepatol Commun"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Liver cirrhosis represents an advanced stage of chronic liver disease and is associated with significant morbidity, mortality, and risk of cancer development. While sex disparity of liver diseases has been observed, understanding at a genetic level awaits more thorough investigation. In this study, we performed a sex\u2010specific analysis of the microRNA (miR) profiles in hepatitis B virus (HBV)\u2013associated cirrhosis by small RNA\u2010sequencing using clinical tissue samples. Potential associated signaling pathways, downstream gene targets, and upstream regulators were highlighted by computational prediction analyses based on the differentially expressed miRs (DEmiRs). From our results, deregulation of miRs in cirrhosis showed a marked difference between males and females by the degree and pattern. Sixty\u2010five (64 up\u2010regulated, 1 down\u2010regulated) and 12 (6 up\u2010regulated, 6 down\u2010regulated) DEmiRs were found in males and females, respectively, when compared with their respective control group. A number of DEmiRs were only observed in one sex but not the other. In addition, 26 DEmiRs were identified between cirrhosis female and cirrhosis male groups. Fatty acid biosynthesis pathway, extracellular matrix\u2013receptor interaction, p53 signaling, Hippo signaling, tumor necrosis factor signaling, the forkhead box O signaling, as well as gene targets ribosomal protein S27 like, methyl CpG binding protein 2, and estrogen receptor 1, may contribute to the pathogenesis and biological behavior of cirrhosis in a sex\u2010specific manner. Analysis of the Cancer Genome Atlas data set suggested a role of sex\u2010specific DEmiRs in multistep hepatocarcinogenesis. <jats:italic toggle=\"yes\">Conclusion<\/jats:italic>: Our findings illustrate that miR profiles in HBV\u2010associated cirrhosis are distinct between the males and females and suggest a potential role of sex\u2010specific biomarkers and molecular mechanisms in disease development and progression.<\/jats:p>","DOI":"10.1002\/hep4.2096","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T15:15:33Z","timestamp":1664291733000},"page":"3473-3486","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Sex\u2010specific analysis of microRNA profiles in HBV\u2010associated cirrhosis by small RNA\u2010sequencing"],"prefix":"10.1097","volume":"6","author":[{"given":"Kristy Kwan\u2010Shuen","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Clinical Medicine,The University of Hong Kong,Hong Kong,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwan\u2010Yung","family":"Au","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Clinical Medicine,The University of Hong Kong,Hong Kong,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai\u2010Ching","family":"Fung","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Clinical Medicine,The University of Hong Kong,Hong Kong,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheuk\u2010Yan","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Clinical Medicine,The University of Hong Kong,Hong Kong,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert Chi\u2010Yan","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Clinical Medicine,The University of Hong Kong,Hong Kong,China"},{"name":"State Key Laboratory of Liver Research (The University of Hong Kong),Hong Kong,China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6213-0343","authenticated-orcid":false,"given":"Regina Cheuk\u2010Lam","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Clinical Medicine,The University of Hong Kong,Hong Kong,China"},{"name":"State Key Laboratory of Liver Research (The University of Hong Kong),Hong Kong,China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","published-online":{"date-parts":[[2022,9,27]]},"reference":[{"key":"hep42096-bib-0001-20241116","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.jhep.2007.11.011","article-title":"Natural history of chronic hepatitis B: special emphasis on disease progression and prognostic factors","volume":"48","year":"2008","journal-title":"J Hepatol"},{"key":"hep42096-bib-0002-20241116","first-page":"283","article-title":"Liver transplantation for hepatitis B virus\u2010related hepatocellular carcinoma in Hong Kong","volume":"6","year":"2018","journal-title":"J Clin Transl Hepatol"},{"key":"hep42096-bib-0003-20241116","doi-asserted-by":"crossref","first-page":"10512","DOI":"10.3748\/wjg.v22.i48.10512","article-title":"Liver fibrosis and hepatic stellate cells: etiology, pathological hallmarks and therapeutic targets","volume":"22","year":"2016","journal-title":"World J Gastroenterol"},{"key":"hep42096-bib-0004-20241116","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/s41586-019-1670-9","article-title":"Somatic mutations and clonal dynamics in healthy and cirrhotic human liver","volume":"574","year":"2019","journal-title":"Nature"},{"key":"hep42096-bib-0005-20241116","first-page":"633","article-title":"Liver disease in women: the influence of gender on epidemiology, natural history, and patient outcomes","volume":"9","year":"2013","journal-title":"Gastroenterol Hepatol (N Y)"},{"key":"hep42096-bib-0006-20241116","article-title":"Sex\u2010dependent outcome of hepatitis B and C viruses infections: synergy of sex hormones and immune responses?","volume":"9","year":"2018","journal-title":"Front Immunol"},{"key":"hep42096-bib-0007-20241116","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s12072-020-10113-2","article-title":"Transition rates to cirrhosis and liver cancer by age, gender, disease and treatment status in Asian chronic hepatitis B patients","volume":"15","year":"2021","journal-title":"Hepatol Int"},{"key":"hep42096-bib-0008-20241116","first-page":"369","article-title":"Eight\u2010year follow\u2010up of the 90,000\u2010person Haimen City cohort: I. 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