{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:13:17Z","timestamp":1790838797777,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,2,11]],"date-time":"2003-02-11T00:00:00Z","timestamp":1044921600000},"content-version":"vor","delay-in-days":41,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hippocampus"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously showed that 16\u2010day\u2010old rats exposed to a relatively high dose of ethanol at 10\u201015 postnatal days of age have fewer neurons in the hilus region of the hippocampus compared with controls. Dentate gyrus granule cell numbers, however, showed no statistically significant changes attributable to the ethanol treatment. It is possible that some of the changes in brain morphology, brought about as a result of the exposure to ethanol during early life, may not be manifested until later in life. This question has been further addressed in an extension to our previous study. Wistar rats were exposed to a relatively high daily dose of ethanol on postnatal days 10\u201315 by placement in a chamber containing ethanol vapour, for 3 h\/day. The blood ethanol concentration was found to be \u223c430 mg\/dl at the end of the period of exposure. Groups of ethanol\u2010treated (ET), separation control (SC), and mother\u2010reared control (MRC) rats were anaesthetised and killed either at 16 or 30 days of age by perfusion with phosphate\u2010buffered 2.5% glutaraldehyde. The Cavalieri principle and the physical disector methods were used to estimate, respectively, the regional volumes and neuron cell numerical densities in the hilus and granule cell regions of the dentate gyrus. The total numbers of neurons in the hilus region and granule cell layer were computed from these estimates. It was found that 16\u2010day\u2010old animals had 398,000\u2013441,000 granule cells, irrespective of group. The numbers of granule cells increased such that by 30 days of age, rats had 487,000\u2013525,500 granule cells. However, there were no significant differences between ethanol\u2010treated rats and their age\u2010matched controls in granule cell numbers. In contrast, ethanol\u2010treated rats had slightly but significantly fewer neurons in the hilus region than did control animals at 16 days of age, but not at 30 days of age. Therefore, it appears that a short period of ethanol exposure during early life can have effects on neuron numbers of some hippocampal neurons, but not others. The effects on hilar neuron numbers, observed as a result of such short periods of ethanol treatment, appeared to be transitory. Hippocampus 2003;13:388\u2013398. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/hipo.10072","type":"journal-article","created":{"date-parts":[[2003,3,14]],"date-time":"2003-03-14T17:00:39Z","timestamp":1047661239000},"page":"388-398","source":"Crossref","is-referenced-by-count":35,"title":["Effects of alcohol exposure during early life on neuron numbers in the rat hippocampus. I. 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