{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T13:08:39Z","timestamp":1773666519811,"version":"3.50.1"},"reference-count":85,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,4,10]],"date-time":"2003-04-10T00:00:00Z","timestamp":1049932800000},"content-version":"vor","delay-in-days":99,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hippocampus"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aging impairs the conduciveness of the lesioned hippocampus for robust survival of neurons derived from homotopic fetal cell grafts (Zaman and Shetty, Neuroscience 109:537\u2013553, 2002), suggesting a need for graft augmentation in fetal graft\u2010mediated therapeutic strategies for the lesioned aging hippocampus. We hypothesize that pre\u2010treatment and grafting of donor hippocampal CA3 cells with fibroblast growth factor\u20102 (FGF\u20102) considerably enhances graft neuronal integration into the lesioned CA3 region of the aging hippocampus. We employed the optical fractionator cell counting method and quantified the number of surviving cells and neurons derived from 5\u2032\u2010bromodoxyuridine\u2010labeled embryonic day 19 CA3 cell grafts pre\u2010treated and transplanted with FGF\u20102 into the lesioned CA3 region of the middle\u2010aged and aged rat hippocampus at 4 days post\u2010lesion. In both middle\u2010aged and aged hippocampus, pre\u2010treatment and transplantation of CA3 cell grafts with FGF\u20102 resulted in a robust yield of surviving cells (72\u201380% of injected cells) and neurons (62\u201369% of injected cells) from grafts. The overall yield was dramatically greater than the yield observed earlier from standard (untreated) fetal CA3 cell grafts into the lesioned aging hippocampus but was highly comparable to that observed for standard fetal CA3 cell grafts into the lesioned young hippocampus (Zaman and Shetty, Neuroscience 109:537\u2013553, 2002). Thus, a robust neuronal integration from fetal CA3 cell grafts can be achieved into the lesioned CA3 region of the aging hippocampus with a simple pre\u2010treatment and grafting of donor fetal CA3 cells with FGF\u20102. These results have implications toward the development of suitable cell grafting strategies for repair of the lesioned aging hippocampus in neurodegenerative diseases, particularly the temporal lobe epilepsy, stroke, and Alzheimer's disease. Hippocampus 2003;13:618\u2013632. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/hipo.10091","type":"journal-article","created":{"date-parts":[[2003,5,12]],"date-time":"2003-05-12T11:32:14Z","timestamp":1052739134000},"page":"618-632","source":"Crossref","is-referenced-by-count":27,"title":["Fetal hippocampal CA3 cell grafts enriched with fibroblast growth factor\u20102 exhibit enhanced neuronal integration into the lesioned aging rat hippocampus in a kainate model of temporal lobe epilepsy"],"prefix":"10.1002","volume":"13","author":[{"given":"Vandana","family":"Zaman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashok K.","family":"Shetty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,4,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903010302"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.1997.14.385"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(91)90082-N"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(85)90299-4"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(97)80314-4"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/75705"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0963-6897(97)00170-X"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490350513"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7123"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/096368970000900205"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970901)385:3<385::AID-CNE4>3.0.CO;2-#"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-21-09519.1999"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1994.tb00247.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780212"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7593"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"5563","DOI":"10.1523\/JNEUROSCI.19-13-05563.1999","article-title":"Diminished viability, growth, and behavioral efficacy of fetal dopamine neuron grafts in aging rats with long\u2010term dopamine depletion: an argument for neurotrophic supplementation","volume":"13","author":"Collier TJ","year":"1999","journal-title":"J Neurosci"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.57.2.260"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000022114"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90133-X"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-4328(94)00134-2"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-14-04996.2001"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002512-199915040-00002"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1997.tb01483.x"},{"key":"e_1_2_1_25_1","first-page":"435","article-title":"Neural transplantation in Parkinson's disease","volume":"86","author":"Freeman TB","year":"2001","journal-title":"Adv Neurol"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.25.11879"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(91)90136-L"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(98)00089-6"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-8428(00)00062-7"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(98)00156-9"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410390317"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1986.tb02764.x"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1987.tb02837.x"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903520111"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199504273321702"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.870130303"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(93)90359-N"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-11-04575.1993"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00228948"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/271676a0"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901920209"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(80)90825-2"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903520404"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.81.4.1645"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(96)80038-5"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(94)90040-X"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-19-08487.1999"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6737"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2001.tb03647.x"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0197-4580(94)90029-9"},{"key":"e_1_2_1_51_1","first-page":"27","article-title":"Fetal hippocampal CA3 cell grafts into the lesioned adult CA3 region restore host hippocampal calbindin expression in a rat model of temporal lobe epilepsy","author":"Shetty AK","year":"2001","journal-title":"Soc Neurosci Abs"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.10031"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(95)00025-E"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)00109-4"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(96)00048-2"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(96)00413-7"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1996.6363"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(19980615)35:4<395::AID-NEU7>3.0.CO;2-U"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19991115)414:2<238::AID-CNE7>3.0.CO;2-A"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7107"},{"key":"e_1_2_1_61_1","first-page":"25","article-title":"Stem cells from fetal hippocampal field CA3 proliferate in response to FGF or EGF, and differentiate into field\u2010specific phenotypic neurons","author":"Shetty AK","year":"1999","journal-title":"Soc Neurosci Abs"},{"key":"e_1_2_1_62_1","doi-asserted-by":"crossref","first-page":"8788","DOI":"10.1523\/JNEUROSCI.20-23-08788.2000","article-title":"Fetal hippocampal transplants containing CA3 Cells restore host hippocampal interneuron numbers in a rat model of temporal lobe epilepsy","volume":"20","author":"Shetty AK","year":"2000","journal-title":"J Neurosci"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1994.1059"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00178-0"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1996.0081"},{"key":"e_1_2_1_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(94)90019-1"},{"key":"e_1_2_1_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1984.tb02501.x"},{"key":"e_1_2_1_68_1","doi-asserted-by":"crossref","first-page":"3932","DOI":"10.1523\/JNEUROSCI.21-11-03932.2001","article-title":"Fibroblast growth factor\u20102 promotes axon branching of cortical neurons by influencing morphology and behavior of the primary growth cone","volume":"21","author":"Szebenyi G","year":"2001","journal-title":"J Neurosci"},{"key":"e_1_2_1_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0195-53"},{"key":"e_1_2_1_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400439"},{"key":"e_1_2_1_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-4328(95)00144-1"},{"key":"e_1_2_1_72_1","first-page":"6","article-title":"Neural transplantation to the normal and lesioned brain: an experimental study of the rat hippocampus","volume":"91","author":"Tonder N","year":"1995","journal-title":"Acta Neurol Scand"},{"key":"e_1_2_1_73_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7411"},{"key":"e_1_2_1_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/77548"},{"key":"e_1_2_1_75_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-09-02786.1991"},{"key":"e_1_2_1_76_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-14-06006.1999"},{"key":"e_1_2_1_77_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08-07-02618.1988"},{"key":"e_1_2_1_78_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092310411"},{"key":"e_1_2_1_79_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.106.1.39"},{"key":"e_1_2_1_80_1","first-page":"663","article-title":"Deafferentation\u2010induced increases in hippocampal IGF\u20101 mRNA expression are severely attenuated in middle aged and aged rats","volume":"83","author":"Woods AG","year":"1998","journal-title":"Neuroscience"},{"key":"e_1_2_1_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)03217-0"},{"key":"e_1_2_1_82_1","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.2001.0440"},{"key":"e_1_2_1_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00478-X"},{"key":"e_1_2_1_84_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7304"},{"key":"e_1_2_1_85_1","doi-asserted-by":"publisher","DOI":"10.3727\/000000001783986963"},{"key":"e_1_2_1_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01408-X"}],"container-title":["Hippocampus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhipo.10091","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hipo.10091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T18:54:30Z","timestamp":1700247270000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hipo.10091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":85,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1002\/hipo.10091"],"URL":"https:\/\/doi.org\/10.1002\/hipo.10091","archive":["Portico"],"relation":{},"ISSN":["1050-9631","1098-1063"],"issn-type":[{"value":"1050-9631","type":"print"},{"value":"1098-1063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]}}}