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The purpose of the present study was to determine whether exposure of rats to ethanol during early postnatal life had either immediate or delayed effects on the numbers of pyramidal cells in the CA1\u2010CA3 subregion of the hippocampus. Wistar rats were exposed to a relatively high daily dose of ethanol at postnatal day 10\u201315 by placing them for 3 h\/day in a chamber containing ethanol vapor. Groups of ethanol\u2010treated (ET), separation control (SC), and mother\u2010reared control (MRC) rats were anesthetized and killed at 16 and 30 days of age by perfusion with phosphate\u2010buffered 2.5% glutaraldehyde. The Cavalieri principle was used to determine the volumes of the CA1 and CA2+CA3 regions. The physical disector method was used to estimate the numerical density of neurons in each of the subdivisions. The total number of pyramidal cells was calculated by multiplying the appropriate estimates of the numerical density by the volume. There were significant age\u2010related reductions in the total numbers of pyramidal cells at 16\u201330 days of age irrespective of the groups examined. Ethanol treated rats were found to have slightly but significantly fewer pyramidal cell neurons than either the MRC or SC groups. These observations indicate that pyramidal cells in the hippocampus may be vulnerable to a relatively high dose of ethanol exposure during this short period of early postnatal life. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/hipo.10155","type":"journal-article","created":{"date-parts":[[2004,2,20]],"date-time":"2004-02-20T15:02:33Z","timestamp":1077289353000},"page":"124-134","source":"Crossref","is-referenced-by-count":43,"title":["Effects of age and alcohol exposure during early life on pyramidal cell numbers in the CA1\u2010CA3 region of the rat hippocampus"],"prefix":"10.1002","volume":"14","author":[{"given":"Takanori","family":"Miki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon J.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter 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