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We investigated whether L\u2010type VDCCs are indeed absent in these large MF boutons. With the use of Sr<jats:sup>2+<\/jats:sup> as the Ca<jats:sup>2+<\/jats:sup> substitute, the stimulus\u2010evoked Sr<jats:sup>2+<\/jats:sup> increment (\u0394[Sr<jats:sup>2+<\/jats:sup>]<jats:sub>pre<\/jats:sub>) was evaluated fluorometrically. \u0394[Sr<jats:sup>2+<\/jats:sup>]<jats:sub>pre<\/jats:sub> appeared to be proportional to Sr<jats:sup>2+<\/jats:sup> inflow through VDCCs and was specifically attenuated by conventional VDCC subtype\u2010selective antagonists. The P\/Q\u2010type selective \u03c9\u2010agatoxin IVA (AgTx<jats:sub>IVA<\/jats:sub>) blocked \u0394[Sr<jats:sup>2+<\/jats:sup>]<jats:sub>pre<\/jats:sub> with an IC<jats:sub>50<\/jats:sub> of 28 nM and by 30\u201335% at its maximum effective concentration of 0.5 \u03bcM. The N\u2010type selective \u03c9\u2010conotoxin GVIA (CgTx<jats:sub>GVIA<\/jats:sub>) blocked \u0394[Sr<jats:sup>2+<\/jats:sup>]<jats:sub>pre<\/jats:sub> with an IC<jats:sub>50<\/jats:sub> of 15 nM and by 20\u201325% at its maximum effective concentration of 1 \u03bcM. The R\u2010type selective SNX\u2010482 blocked \u0394[Sr<jats:sup>2+<\/jats:sup>]<jats:sub>pre<\/jats:sub> with an IC<jats:sub>50<\/jats:sub> of 79 nM and by 20\u201325% at its maximum effective concentration of 1 \u03bcM. The effects of these toxins did not overlap at their maximum effective concentrations and about 70\u201380% of \u0394[Sr<jats:sup>2+<\/jats:sup>]<jats:sub>pre<\/jats:sub> was blocked by the simultaneous exposure to these toxins. \u0394[Sr<jats:sup>2+<\/jats:sup>]<jats:sub>pre<\/jats:sub> component that is resistant to AgTx<jats:sub>IVA<\/jats:sub>, CgTx<jats:sub>GVIA<\/jats:sub>, and SNX\u2010482 was significantly potentiated by an L\u2010type agonist, (S)\u2010(\u2212)\u2010Bay K8644, and attenuated by an L\u2010type antagonist, nimodipine, suggesting that L\u2010type VDCCs are present in large MF terminals. The L\u2010type agonist, (\u00b1)\u2010Bay K8644, also potentiated Sr<jats:sup>2+<\/jats:sup> inflow into individual boutons identified as large MF boutons under confocal microscopy. Almost similar results were observed for Ca<jats:sup>2+<\/jats:sup> inflow\u2010dependent fluorescence increments. L\u2010type VDCCs appear to be present in large MF boutons and mediate a substantial Ca<jats:sup>2+<\/jats:sup> inflow into presynaptic terminals during action potentials. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/hipo.10202","type":"journal-article","created":{"date-parts":[[2004,4,6]],"date-time":"2004-04-06T18:35:32Z","timestamp":1081276532000},"page":"570-585","source":"Crossref","is-referenced-by-count":22,"title":["Pharmacological dissection of calcium channel subtype\u2010related components of strontium inflow in large mossy fiber boutons of mouse 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