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Brain amyloid deposits are classical histopathological hallmarks that generate an inflammatory reaction affecting neuronal and glial function. The identification of early cell responses and of brain areas involved could help to design new successful treatments. Hence, we studied early alterations of hippocampal glia and their progression during the neuropathology in PDAPP\u2010J20 transgenic mice, AD model, at 3, 9, and 15 months (m) of age. At 3 m, before deposits formation, microglial Iba1+ cells from transgenic mice already exhibited signs of activation and larger soma size in the hilus, alterations appearing later on stratum radiatum. Iba1 immunohistochemistry revealed increased cell density and immunoreactive area in PDAPP mice from 9 m onward selectively in the hilus, in coincidence with prominent amyloid Congo red\u2009+\u2009deposition. At pre\u2010plaque stages, GFAP+ astroglia showed density alterations while, at an advanced age, the presence of deposits was associated with important glial volume changes and apparently being intimately involved in amyloid degradation. Astrocytes around plaques were strongly labeled for LC3 until 15 m in Tg mice, suggestive of increased autophagic flux. Moreover, \u03b2\u2010Amyloid fibrils internalization by astrocytes in in vitro conditions was dependent on autophagy. Co\u2010localization of Iba1 with ubiquitin or p62 was exclusively found in microglia contacting deposits from 9 m onward, suggesting torpid autophagy. Our work characterizes glial changes at early stages of the disease in PDAPP\u2010J20 mice, focusing on the hilus as an especially susceptible hippocampal subfield, and provides evidence that glial autophagy could play a role in amyloid processing at advanced stages. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/hipo.22503","type":"journal-article","created":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T08:45:48Z","timestamp":1438418748000},"page":"194-210","source":"Crossref","is-referenced-by-count":77,"title":["Glial alterations from early to late stages in a model of<scp>A<\/scp>lzheimer's disease: Evidence of autophagy involvement in<scp>A<\/scp>\u03b2 internalization"],"prefix":"10.1002","volume":"26","author":[{"given":"Carlos","family":"Pomilio","sequence":"first","affiliation":[{"name":"Departamento De Quimica Biologica Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires, Instituto De Biologia Y Medicina Experimental Conicet Buenos Aires 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