{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:42:41Z","timestamp":1790998961598,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T00:00:00Z","timestamp":1726185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000096","name":"Scottish Rite Charitable Foundation of Canada","doi-asserted-by":"publisher","award":["21107"],"award-info":[{"award-number":["21107"]}],"id":[{"id":"10.13039\/501100000096","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hippocampus"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fragile\u2010X Syndrome (FXS)\u00a0is the leading monogenetic cause of intellectual disability among children but remains without a cure. Using the <jats:italic>Fmr1<\/jats:italic> <jats:italic>KO<\/jats:italic> mouse model of FXS, much work has been done to understand FXS hippocampus dysfunction. Purinergic signaling, where ATP and its metabolites are used as signaling molecules, participates in hippocampus development, but it is unknown if purinergic signaling is affected in the developing <jats:italic>Fmr1<\/jats:italic> <jats:italic>KO<\/jats:italic> hippocampus. In our study, we characterized the purinergic receptor P2X7. We first found that P2X7 was reduced in <jats:italic>Fmr1 KO<\/jats:italic> whole hippocampus tissue at P14 and P21, corresponding to the periods of neurite outgrowth and synaptic refinement in the hippocampus. We then evaluated the cell\u2010specific expression of P2X7 with immunofluorescence\u00a0and found differences between WT and <jats:italic>Fmr1 KO<\/jats:italic> mice in P2X7 colocalization with hippocampal microglia and neurons. P2X7 colocalized more with microglia at P14 and P21, but there was a sex\u2010specific reduction in P2X7 colocalization with neurons. In contrast, male mice at P14 and P21 showed reduced neuronal P2X7 colocalization compared to females, but only females showed reduced absolute neuronal P2X7 expression across the dorsal hippocampal formation. Together, our results suggest that P2X7 expression is altered during <jats:italic>Fmr1<\/jats:italic>\u2010KO hippocampal development, potentially influencing several developmental processes in the <jats:italic>Fmr1\u2010<\/jats:italic>KO hippocampus formation.<\/jats:p>","DOI":"10.1002\/hipo.23634","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T17:14:12Z","timestamp":1726247652000},"page":"633-644","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>P2X7<\/scp> expression patterns in the developing <i>Fmr1<\/i>\u2010knockout mouse hippocampus"],"prefix":"10.1002","volume":"34","author":[{"given":"Matthew","family":"Napier","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Ontario Canada"},{"name":"Department of Molecular and Cellular Biology University of Guelph  Guelph Ontario 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