{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:52:19Z","timestamp":1759683139841},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T00:00:00Z","timestamp":1097625600000},"content-version":"vor","delay-in-days":4392,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hippocampus"],"published-print":{"date-parts":[[1992,10,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The NMDA receptor\u2010mediated component of the hippocampal granule cell population excitatory postsynaptic potential response to low frequency (&lt; 0.2 Hz) stimulation of the medial perforant path was characterized <jats:italic>in vivo<\/jats:italic>. Extracellular recordings were obtained from the dentate molecular layer in anesthetized rabbits, and glutamatergic and GABAergic antagonists were applied locally by pressure ejection. To measure the NMDA\u2010mediated component, the NMDA receptor antagonist <jats:sc>D<\/jats:sc>\u2010;5\u2010aminophosphonovalerate (APV) was applied during the constant ejection of physiological saline, 6\u2010cyano\u20107\u2010nitroquinoxaline\u20102, 3\u2010dione (CNQX), and\/or bicuculline methiodide. In general agreement with the results of attempts by other investigators to identify NMDA responses <jats:italic>in vivo<\/jats:italic>, APV did not significantly reduce the response to a single stimulus impulse in the presence of saline. However, an NMDA\u2010mediated response was revealed when \u03b1\u2010amino\u20103\u2010hydroxy\u20105\u2010methyl\u20104\u2010isoxazoleproprianate receptor\u2010mediated current flow was eliminated by applying the non\u2010NMDA receptor antagonist CNQX. The NMDA component was negative\u2010going as predicted, but its duration was considerably less than indicated in other studies of the dentate <jats:italic>in vitro<\/jats:italic>. The relative magnitudes of the NMDA and non\u2010NMDA components of the EPSP were found to vary as a function of stimulus intensity or frequency. The NMDA receptor\u2010mediated component represented 12% of the control response and increased to over 25% in response to higher stimulus intensities. A brief, high\u2010frequency burst of impulses evoked a larger NMDA component in the presence of CNQX and was able to evoke an NMDA component in the presence of saline. Surprisingly, short trains of stimulation at lower frequencies typically produced suppression of the NMDA component. In a final series of experiments, it was found that many characteristics of the NMDA component were substantially altered by GABAergic inhibition. In the presence of the GABA<jats:sub>A<\/jats:sub> antagonist bicuculline, the magnitude of NMDA receptor\u2010mediated responses was increased and their duration was greatly extended. Additionally, in the presence of bicuculline, the NMDA component facilitated markedly in response to frequencies of stimulus input &gt;20 Hz.<\/jats:p><jats:p>These results indicate <jats:italic>in vivo<\/jats:italic> that the initiation and duration of NMDA current flow depend strongly upon the intensity and frequency of perforant path stimulation. In addition, the NMDA response to a single impulse appears to be reduced and truncated by input from GABA<jats:sub>A<\/jats:sub> receptormediated feedback and\/or feedforward inhibition, and this inhibition affects temporal summation of NMDA receptor\u2010mediated responses over a wide range of input frequencies. It is suggested that such inhibition results from the activation of GABA<jats:sub>A<\/jats:sub> receptors located on granule cell dendritic shafts. Thus, conditions that reduce the firing rate or effect of GABAergic interneurons will be likely to augment both the amplitude and duration of NMDA receptor\u2010mediated Ca<jats:sup>2+<\/jats:sup> influx and to alter the types of afferent patterns that are effective in activating postsynaptic NMDA receptors.<\/jats:p>","DOI":"10.1002\/hipo.450020405","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T12:26:42Z","timestamp":1117369602000},"page":"373-388","source":"Crossref","is-referenced-by-count":14,"title":["Characterization <i>in vivo<\/i> of the NMDA receptor\u2010mediated component of dentate granule cell population synaptic responses to perforant path input"],"prefix":"10.1002","volume":"2","author":[{"given":"Thomas A.","family":"Blanpied","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodore W.","family":"Berger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90477-6"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90582-3"},{"key":"e_1_2_1_4_1","first-page":"381","volume-title":"Brain Slices","author":"Alger B.","year":"1984"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1966.tb03223.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017690"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-05-01452.1986"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-9789-2_6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1973.sp010273"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1991.tb00062.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.5.2516"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90214-9"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.13.030190.001131"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(85)90091-6"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017080"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014478"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1139\/y84-067"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014829"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00232206"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1983.sp014899"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(87)90019-4"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)90275-0"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90275-9"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90162-9"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2899909"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90117-M"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/325529a0"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00228158"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018510"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00247498"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1990.64.1.119"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(89)90184-X"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)91388-1"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902950110"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/346565a0"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90538-0"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00234414"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/321519a0"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90575-3"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/309261a0"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1985.53.2.557"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(90)90011-C"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901750404"},{"key":"e_1_2_1_44_1","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1152\/jn.1981.46.5.952","article-title":"Synaptic efficacy and EPSP summation in granule cells of rat fascia dentata studied in vitro","volume":"46","author":"McNaughton B. L.","year":"1981","journal-title":"J. Neurophysiol."},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/326701a0"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-11-02909.1985"},{"key":"e_1_2_1_47_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1523\/JNEUROSCI.11-01-00203.1991","article-title":"GABAB receptor mediated inhibition of the N\u2010methyl\u2010d\u2010aspartate component of synaptic transmission in the rat hippocampus","volume":"11","author":"Morrisett R. A.","year":"1991","journal-title":"J. Neuosci."},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1675489"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890050203"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/307462a0"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890070102"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90714-M"},{"key":"e_1_2_1_53_1","first-page":"246","article-title":"GABAergic contribution to the nonlinear response properties of the in vivo dentate gyrus","volume":"18","author":"Port R. L.","year":"1988","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(90)90280-5"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01181525"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(83)91306-9"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90100-I"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902930208"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.04-10-02590.1984"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/301517a0"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(78)91113-7"},{"key":"e_1_2_1_62_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018453"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90615-I"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018304"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00236566"},{"key":"e_1_2_1_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/328640a0"},{"key":"e_1_2_1_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(86)90080-1"},{"key":"e_1_2_1_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902800207"},{"key":"e_1_2_1_69_1","doi-asserted-by":"crossref","first-page":"3230","DOI":"10.1523\/JNEUROSCI.09-09-03230.1989","article-title":"Quantitative physiological characterization of a quinoxalinedione non\u2010NMDA receptor antagonist","volume":"9","author":"Yamada K. A.","year":"1989","journal-title":"J. Neurosci."},{"key":"e_1_2_1_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)91231-6"}],"container-title":["Hippocampus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhipo.450020405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hipo.450020405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T14:44:13Z","timestamp":1695825853000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hipo.450020405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10,4]]},"references-count":69,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,10,4]]}},"alternative-id":["10.1002\/hipo.450020405"],"URL":"https:\/\/doi.org\/10.1002\/hipo.450020405","archive":["Portico"],"relation":{},"ISSN":["1050-9631","1098-1063"],"issn-type":[{"value":"1050-9631","type":"print"},{"value":"1098-1063","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10,4]]}}}