{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:37:10Z","timestamp":1792147030679,"version":"4.4.0"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,12,22]],"date-time":"2011-12-22T00:00:00Z","timestamp":1324512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hairy cell leukaemia (HCL) is a rare type of B\u2010cell non\u2010Hodgkin lymphoma (B\u2010NHL), which is not known to be associated with any characteristic recurrent karyotypic abnormality. A recent study that used massively parallel whole exome sequencing identified an activating V600E mutation in BRAF, which appeared specific for HCL. Here, we confirm the specificity of BRAF V600E for HCL among low and intermediate grade B\u2010NHL and describe a real\u2010time polymerase chain reaction method for detecting this mutation in cases with low tumour burden. The V600E mutation does not appear to be associated with microsatellite instability, unlike the case in colorectal cancer. Thus, in conjunction with prior data, our results suggest incorporation of BRAF V600E mutation analysis in the diagnostic workup of HCL cases. Additionally, targeting the Ras\u2010Raf\u2010Mek\u2010Erk\u2010Map kinase pathway should be investigated as a potential therapeutic strategy for patients with this disease. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/hon.1023","type":"journal-article","created":{"date-parts":[[2011,12,23]],"date-time":"2011-12-23T03:18:38Z","timestamp":1324610318000},"page":"190-193","source":"Crossref","is-referenced-by-count":17,"title":["Real\u2010time PCR\u2010based analysis of BRAF V600E mutation in low and intermediate grade lymphomas confirms frequent occurrence in hairy cell leukaemia"],"prefix":"10.1002","volume":"30","author":[{"given":"Mark","family":"Ewalt","sequence":"first","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center and New York Presbyterian Hospital  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhadra","family":"Nandula","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center and New York Presbyterian Hospital  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienne","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Hematology and Oncology Columbia University Medical Center and New York Presbyterian Hospital  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bachir","family":"Alobeid","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center and New York Presbyterian Hospital  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vundavalli V.","family":"Murty","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center and New York Presbyterian Hospital  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahesh M.","family":"Mansukhani","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center and New York Presbyterian Hospital  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Govind","family":"Bhagat","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Medical Center and New York Presbyterian Hospital  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1014209"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00766"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6601371"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09837"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08868.x"},{"key":"e_1_2_8_7_1","first-page":"188","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Foucar K","year":"2008"},{"issue":"3","key":"e_1_2_8_8_1","first-page":"500","article-title":"DNA extraction from fixed cytogenetic cell suspensions","volume":"6","author":"Amorim MR","year":"2007","journal-title":"Genet Mol Res"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.060070"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10310"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24681"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428190903142208"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e328338c417"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1888"}],"container-title":["Hematological Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhon.1023","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.1023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T12:13:06Z","timestamp":1700568786000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hon.1023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,22]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1002\/hon.1023"],"URL":"https:\/\/doi.org\/10.1002\/hon.1023","archive":["Portico"],"relation":{},"ISSN":["0278-0232","1099-1069"],"issn-type":[{"value":"0278-0232","type":"print"},{"value":"1099-1069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,22]]}}}